Experiment: MoYLS4 with Antimycin A 1uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnE and phnE overlap by 4 nucleotides phnE and phnC overlap by 13 nucleotides phnC and DVU1937 are separated by 76 nucleotides
DVU1934: phnE - phosphonate ABC transporter, permease protein (TIGR), at 2,007,149 to 2,007,949
phnE
DVU1935: phnE - phosphonate ABC transporter, permease protein (TIGR), at 2,007,946 to 2,008,743
phnE
DVU1936: phnC - phosphonate ABC transporter, ATP-binding protein (TIGR), at 2,008,731 to 2,009,528
phnC
DVU1937: DVU1937 - phosphonate ABC transporter, periplasmic phosphonate-binding protein, putative (TIGR), at 2,009,605 to 2,010,618
DVU1937
Position (kb)
2008
2009
2010 Strain fitness (log2 ratio)
-1
0
1
2 at 2007.838 kb on - strand, within phnE at 2007.840 kb on - strand, within phnE at 2008.138 kb on - strand, within phnE at 2008.186 kb on + strand, within phnE at 2008.196 kb on + strand, within phnE at 2008.435 kb on + strand, within phnE at 2008.476 kb on + strand, within phnE at 2008.556 kb on + strand, within phnE at 2008.684 kb on - strand at 2008.912 kb on + strand, within phnC at 2009.256 kb on + strand, within phnC at 2009.279 kb on - strand, within phnC at 2009.291 kb on - strand, within phnC at 2009.397 kb on - strand, within phnC at 2009.416 kb on - strand, within phnC at 2009.445 kb on + strand, within phnC at 2009.455 kb on + strand at 2009.463 kb on - strand at 2009.528 kb on + strand at 2009.674 kb on + strand at 2009.711 kb on + strand, within DVU1937 at 2009.940 kb on - strand, within DVU1937 at 2009.997 kb on + strand, within DVU1937 at 2010.005 kb on - strand, within DVU1937 at 2010.005 kb on - strand, within DVU1937 at 2010.023 kb on + strand, within DVU1937 at 2010.023 kb on + strand, within DVU1937 at 2010.045 kb on + strand, within DVU1937 at 2010.116 kb on + strand, within DVU1937 at 2010.116 kb on + strand, within DVU1937 at 2010.122 kb on + strand, within DVU1937 at 2010.144 kb on + strand, within DVU1937 at 2010.149 kb on + strand, within DVU1937 at 2010.193 kb on - strand, within DVU1937 at 2010.247 kb on + strand, within DVU1937 at 2010.271 kb on - strand, within DVU1937 at 2010.273 kb on - strand, within DVU1937 at 2010.310 kb on - strand, within DVU1937 at 2010.317 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Antimycin A 1uM remove 2,007,838 - phnE DVU1934 0.86 -0.1 2,007,840 - phnE DVU1934 0.86 -1.6 2,008,138 - phnE DVU1935 0.24 -0.0 2,008,186 + phnE DVU1935 0.30 -1.4 2,008,196 + phnE DVU1935 0.31 +0.4 2,008,435 + phnE DVU1935 0.61 -0.2 2,008,476 + phnE DVU1935 0.66 -0.3 2,008,556 + phnE DVU1935 0.76 +0.1 2,008,684 - -0.4 2,008,912 + phnC DVU1936 0.23 -1.3 2,009,256 + phnC DVU1936 0.66 -0.4 2,009,279 - phnC DVU1936 0.69 -0.0 2,009,291 - phnC DVU1936 0.70 -0.7 2,009,397 - phnC DVU1936 0.83 -0.1 2,009,416 - phnC DVU1936 0.86 +0.5 2,009,445 + phnC DVU1936 0.89 +0.0 2,009,455 + -0.2 2,009,463 - -0.2 2,009,528 + -0.4 2,009,674 + -0.1 2,009,711 + DVU1937 0.10 -0.3 2,009,940 - DVU1937 0.33 -0.2 2,009,997 + DVU1937 0.39 +1.7 2,010,005 - DVU1937 0.39 +0.1 2,010,005 - DVU1937 0.39 -1.0 2,010,023 + DVU1937 0.41 -0.3 2,010,023 + DVU1937 0.41 -1.3 2,010,045 + DVU1937 0.43 -0.2 2,010,116 + DVU1937 0.50 -0.2 2,010,116 + DVU1937 0.50 +0.9 2,010,122 + DVU1937 0.51 -0.0 2,010,144 + DVU1937 0.53 +0.1 2,010,149 + DVU1937 0.54 +2.4 2,010,193 - DVU1937 0.58 +0.1 2,010,247 + DVU1937 0.63 -0.0 2,010,271 - DVU1937 0.66 -1.5 2,010,273 - DVU1937 0.66 -0.3 2,010,310 - DVU1937 0.70 +0.6 2,010,317 + DVU1937 0.70 -0.1 2,010,332 + DVU1937 0.72 +0.7 2,010,332 + DVU1937 0.72 +0.1 2,010,332 + DVU1937 0.72 -0.0 2,010,340 - DVU1937 0.72 -0.6 2,010,340 - DVU1937 0.72 -1.4 2,010,340 - DVU1937 0.72 -0.2
Or see this region's nucleotide sequence