Experiment: Ceftazidime 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and tRNA-Gly are separated by 157 nucleotides tRNA-Gly and ribH are separated by 61 nucleotides ribH and psbZ are separated by 9 nucleotides psbZ and Synpcc7942_2246 are separated by 206 nucleotides
Synpcc7942_2243: proA - Glutamate-5-semialdehyde dehydrogenase, at 2,308,253 to 2,309,497
proA
Synpcc7942_R0009: tRNA-Gly - tRNA-Gly, at 2,309,655 to 2,309,726
tRNA-Gly
Synpcc7942_2244: ribH - riboflavin synthase subunit beta, at 2,309,788 to 2,310,333
ribH
Synpcc7942_2245: psbZ - photosystem II reaction center protein PsbZ, at 2,310,343 to 2,310,531
psbZ
Synpcc7942_2246: Synpcc7942_2246 - hypothetical protein, at 2,310,738 to 2,312,300
_2246
Position (kb)
2310
2311 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2309.387 kb on + strand at 2309.395 kb on - strand at 2309.399 kb on + strand at 2309.407 kb on + strand at 2309.427 kb on + strand at 2309.428 kb on + strand at 2309.434 kb on - strand at 2309.436 kb on - strand at 2309.457 kb on + strand at 2309.557 kb on + strand at 2309.565 kb on - strand at 2309.569 kb on + strand at 2309.574 kb on + strand at 2309.577 kb on - strand at 2309.577 kb on - strand at 2309.584 kb on - strand at 2310.389 kb on + strand, within psbZ at 2310.497 kb on + strand, within psbZ at 2310.545 kb on + strand at 2310.665 kb on - strand at 2310.680 kb on + strand at 2310.685 kb on + strand at 2310.692 kb on - strand at 2310.773 kb on - strand at 2310.822 kb on + strand at 2310.845 kb on + strand at 2310.845 kb on + strand at 2310.845 kb on + strand at 2310.845 kb on + strand at 2310.845 kb on + strand at 2310.853 kb on - strand at 2310.853 kb on - strand at 2310.853 kb on - strand at 2310.853 kb on - strand at 2310.867 kb on - strand at 2310.869 kb on + strand at 2310.911 kb on + strand, within Synpcc7942_2246 at 2310.924 kb on - strand, within Synpcc7942_2246 at 2310.935 kb on + strand, within Synpcc7942_2246 at 2310.942 kb on + strand, within Synpcc7942_2246 at 2310.942 kb on + strand, within Synpcc7942_2246 at 2310.942 kb on + strand, within Synpcc7942_2246 at 2310.950 kb on - strand, within Synpcc7942_2246 at 2310.972 kb on + strand, within Synpcc7942_2246 at 2310.984 kb on + strand, within Synpcc7942_2246 at 2310.984 kb on + strand, within Synpcc7942_2246 at 2311.030 kb on + strand, within Synpcc7942_2246 at 2311.030 kb on + strand, within Synpcc7942_2246 at 2311.038 kb on - strand, within Synpcc7942_2246 at 2311.092 kb on - strand, within Synpcc7942_2246 at 2311.144 kb on + strand, within Synpcc7942_2246 at 2311.144 kb on + strand, within Synpcc7942_2246 at 2311.200 kb on + strand, within Synpcc7942_2246 at 2311.210 kb on + strand, within Synpcc7942_2246 at 2311.210 kb on + strand, within Synpcc7942_2246 at 2311.213 kb on - strand, within Synpcc7942_2246 at 2311.330 kb on + strand, within Synpcc7942_2246 at 2311.330 kb on + strand, within Synpcc7942_2246 at 2311.345 kb on + strand, within Synpcc7942_2246 at 2311.357 kb on + strand, within Synpcc7942_2246 at 2311.392 kb on - strand, within Synpcc7942_2246 at 2311.433 kb on - strand, within Synpcc7942_2246 at 2311.439 kb on + strand, within Synpcc7942_2246 at 2311.439 kb on + strand, within Synpcc7942_2246 at 2311.475 kb on + strand, within Synpcc7942_2246 at 2311.480 kb on + strand, within Synpcc7942_2246 at 2311.493 kb on + strand, within Synpcc7942_2246 at 2311.500 kb on + strand, within Synpcc7942_2246 at 2311.520 kb on - strand, within Synpcc7942_2246
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 0.125 mg/ml remove 2,309,387 + +3.2 2,309,395 - -0.6 2,309,399 + +5.7 2,309,407 + +4.6 2,309,427 + +0.5 2,309,428 + -2.1 2,309,434 - +0.9 2,309,436 - +3.5 2,309,457 + -0.2 2,309,557 + +0.5 2,309,565 - +2.4 2,309,569 + +1.7 2,309,574 + +1.8 2,309,577 - +0.5 2,309,577 - +0.9 2,309,584 - +1.8 2,310,389 + psbZ Synpcc7942_2245 0.24 -0.1 2,310,497 + psbZ Synpcc7942_2245 0.81 +1.2 2,310,545 + +0.9 2,310,665 - -0.3 2,310,680 + -0.6 2,310,685 + +1.3 2,310,692 - +4.2 2,310,773 - +1.7 2,310,822 + +4.7 2,310,845 + +3.9 2,310,845 + +2.3 2,310,845 + +1.4 2,310,845 + +0.9 2,310,845 + +0.5 2,310,853 - -1.4 2,310,853 - +6.9 2,310,853 - +0.9 2,310,853 - -0.9 2,310,867 - -0.6 2,310,869 + -1.1 2,310,911 + Synpcc7942_2246 0.11 +0.7 2,310,924 - Synpcc7942_2246 0.12 +2.3 2,310,935 + Synpcc7942_2246 0.13 +3.2 2,310,942 + Synpcc7942_2246 0.13 -0.1 2,310,942 + Synpcc7942_2246 0.13 +4.4 2,310,942 + Synpcc7942_2246 0.13 -1.6 2,310,950 - Synpcc7942_2246 0.14 +3.6 2,310,972 + Synpcc7942_2246 0.15 -0.1 2,310,984 + Synpcc7942_2246 0.16 -1.6 2,310,984 + Synpcc7942_2246 0.16 +0.9 2,311,030 + Synpcc7942_2246 0.19 -1.6 2,311,030 + Synpcc7942_2246 0.19 -1.6 2,311,038 - Synpcc7942_2246 0.19 -2.2 2,311,092 - Synpcc7942_2246 0.23 +5.1 2,311,144 + Synpcc7942_2246 0.26 +2.6 2,311,144 + Synpcc7942_2246 0.26 +5.5 2,311,200 + Synpcc7942_2246 0.30 +1.6 2,311,210 + Synpcc7942_2246 0.30 -0.6 2,311,210 + Synpcc7942_2246 0.30 +3.9 2,311,213 - Synpcc7942_2246 0.30 +0.9 2,311,330 + Synpcc7942_2246 0.38 -1.1 2,311,330 + Synpcc7942_2246 0.38 -0.6 2,311,345 + Synpcc7942_2246 0.39 -0.1 2,311,357 + Synpcc7942_2246 0.40 -0.6 2,311,392 - Synpcc7942_2246 0.42 -1.1 2,311,433 - Synpcc7942_2246 0.44 +1.8 2,311,439 + Synpcc7942_2246 0.45 +6.3 2,311,439 + Synpcc7942_2246 0.45 +3.7 2,311,475 + Synpcc7942_2246 0.47 -1.6 2,311,480 + Synpcc7942_2246 0.47 +0.5 2,311,493 + Synpcc7942_2246 0.48 +0.2 2,311,500 + Synpcc7942_2246 0.49 +5.5 2,311,520 - Synpcc7942_2246 0.50 +3.9
Or see this region's nucleotide sequence