Experiment: butyrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5289 and Pf1N1B4_5290 are separated by 351 nucleotides Pf1N1B4_5290 and Pf1N1B4_5291 are separated by 290 nucleotides Pf1N1B4_5291 and Pf1N1B4_5292 are separated by 986 nucleotides
Pf1N1B4_5289 - hypothetical protein, at 43,736 to 44,062
_5289
Pf1N1B4_5290 - hypothetical protein, at 44,414 to 44,644
_5290
Pf1N1B4_5291 - Putative reductase, at 44,935 to 46,248
_5291
Pf1N1B4_5292 - hypothetical protein, at 47,235 to 47,396
_5292
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 43.944 kb on + strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 44.127 kb on - strand at 44.255 kb on + strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.275 kb on + strand at 44.415 kb on + strand at 44.650 kb on - strand at 45.101 kb on + strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.201 kb on - strand at 45.201 kb on - strand, within Pf1N1B4_5291 at 45.347 kb on + strand, within Pf1N1B4_5291 at 45.347 kb on + strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.452 kb on + strand, within Pf1N1B4_5291 at 45.465 kb on - strand, within Pf1N1B4_5291 at 45.500 kb on + strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.598 kb on + strand, within Pf1N1B4_5291 at 45.650 kb on + strand, within Pf1N1B4_5291 at 45.651 kb on - strand, within Pf1N1B4_5291 at 45.902 kb on + strand, within Pf1N1B4_5291 at 45.943 kb on + strand at 46.007 kb on + strand, within Pf1N1B4_5291 at 46.231 kb on + strand at 46.256 kb on - strand at 46.256 kb on - strand at 46.325 kb on + strand at 46.326 kb on - strand at 46.389 kb on - strand at 46.421 kb on + strand at 46.422 kb on - strand at 46.532 kb on - strand at 46.542 kb on + strand at 46.574 kb on - strand at 46.574 kb on - strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.844 kb on + strand at 46.844 kb on + strand at 46.845 kb on - strand at 46.927 kb on + strand at 47.002 kb on + strand at 47.002 kb on + strand at 47.002 kb on + strand at 47.018 kb on + strand at 47.019 kb on - strand at 47.032 kb on + strand at 47.032 kb on + strand at 47.032 kb on + strand at 47.033 kb on - strand at 47.066 kb on + strand at 47.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction butyrate (C) remove 43,944 + Pf1N1B4_5289 0.64 +0.1 43,945 - Pf1N1B4_5289 0.64 -0.9 43,945 - Pf1N1B4_5289 0.64 +0.1 43,967 - Pf1N1B4_5289 0.71 -1.3 43,967 - Pf1N1B4_5289 0.71 -0.5 44,127 - +0.9 44,255 + +0.1 44,256 - +0.1 44,256 - +0.5 44,256 - +0.5 44,275 + -0.5 44,415 + -0.4 44,650 - -0.7 45,101 + Pf1N1B4_5291 0.13 -1.5 45,102 - Pf1N1B4_5291 0.13 +0.4 45,102 - Pf1N1B4_5291 0.13 -0.8 45,102 - Pf1N1B4_5291 0.13 -2.3 45,132 - Pf1N1B4_5291 0.15 -0.5 45,132 - Pf1N1B4_5291 0.15 +0.1 45,132 - Pf1N1B4_5291 0.15 -0.2 45,201 - +0.1 45,201 - Pf1N1B4_5291 0.20 +0.1 45,347 + Pf1N1B4_5291 0.31 -1.7 45,347 + Pf1N1B4_5291 0.31 +1.6 45,348 - Pf1N1B4_5291 0.31 +0.1 45,348 - Pf1N1B4_5291 0.31 +0.5 45,348 - Pf1N1B4_5291 0.31 +0.0 45,452 + Pf1N1B4_5291 0.39 -0.2 45,465 - Pf1N1B4_5291 0.40 -0.4 45,500 + Pf1N1B4_5291 0.43 -3.7 45,501 - Pf1N1B4_5291 0.43 -1.9 45,501 - Pf1N1B4_5291 0.43 -0.4 45,501 - Pf1N1B4_5291 0.43 -0.3 45,598 + Pf1N1B4_5291 0.50 +1.6 45,650 + Pf1N1B4_5291 0.54 -0.1 45,651 - Pf1N1B4_5291 0.54 +1.4 45,902 + Pf1N1B4_5291 0.74 -0.7 45,943 + +0.1 46,007 + Pf1N1B4_5291 0.82 -2.9 46,231 + +0.1 46,256 - +0.1 46,256 - +0.1 46,325 + +0.8 46,326 - +0.1 46,389 - +1.1 46,421 + -0.2 46,422 - -0.9 46,532 - -1.9 46,542 + +0.1 46,574 - +0.3 46,574 - +0.3 46,786 + +0.6 46,786 + -0.9 46,786 + +1.3 46,786 + +2.6 46,786 + -0.3 46,787 - -0.5 46,787 - -1.9 46,787 - +0.3 46,844 + -0.5 46,844 + +0.3 46,845 - +2.6 46,927 + -0.4 47,002 + +0.8 47,002 + +2.4 47,002 + +0.3 47,018 + -0.4 47,019 - +1.2 47,032 + +0.9 47,032 + +0.2 47,032 + -0.4 47,033 - -0.4 47,066 + -0.5 47,204 + +0.1
Or see this region's nucleotide sequence