Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3258 and Dsui_3259 are separated by 138 nucleotides Dsui_3259 and Dsui_3260 are separated by 65 nucleotides Dsui_3260 and Dsui_3261 are separated by 116 nucleotides
Dsui_3258: Dsui_3258 - mannose-6-phosphate isomerase, at 3,497,713 to 3,498,105
_3258
Dsui_3259: Dsui_3259 - dehydrogenase of unknown specificity, short-chain alcohol dehydrogenase like protein, at 3,498,244 to 3,499,014
_3259
Dsui_3260: Dsui_3260 - vacuolar-type H(+)-translocating pyrophosphatase, at 3,499,080 to 3,501,149
_3260
Dsui_3261: Dsui_3261 - 6-phosphofructokinase, at 3,501,266 to 3,502,534
_3261
Position (kb)
3499
3500
3501
3502 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3498.106 kb on - strand at 3498.145 kb on + strand at 3498.247 kb on + strand at 3498.332 kb on + strand, within Dsui_3259 at 3498.517 kb on + strand, within Dsui_3259 at 3498.522 kb on - strand at 3498.552 kb on - strand at 3498.696 kb on - strand, within Dsui_3259 at 3498.744 kb on + strand, within Dsui_3259 at 3498.764 kb on + strand, within Dsui_3259 at 3498.770 kb on + strand, within Dsui_3259 at 3498.770 kb on + strand, within Dsui_3259 at 3498.778 kb on - strand at 3498.920 kb on + strand, within Dsui_3259 at 3498.940 kb on + strand at 3498.941 kb on - strand at 3499.077 kb on + strand at 3499.078 kb on + strand at 3499.168 kb on - strand at 3499.205 kb on + strand at 3499.306 kb on + strand at 3499.355 kb on - strand, within Dsui_3260 at 3499.363 kb on + strand at 3499.531 kb on - strand, within Dsui_3260 at 3499.588 kb on + strand, within Dsui_3260 at 3499.663 kb on - strand, within Dsui_3260 at 3499.702 kb on - strand, within Dsui_3260 at 3499.734 kb on - strand, within Dsui_3260 at 3499.752 kb on - strand, within Dsui_3260 at 3499.778 kb on + strand at 3499.814 kb on + strand at 3499.847 kb on + strand at 3499.889 kb on + strand, within Dsui_3260 at 3499.993 kb on + strand at 3500.070 kb on + strand, within Dsui_3260 at 3500.078 kb on - strand, within Dsui_3260 at 3500.125 kb on - strand, within Dsui_3260 at 3500.125 kb on - strand, within Dsui_3260 at 3500.125 kb on - strand, within Dsui_3260 at 3500.196 kb on - strand, within Dsui_3260 at 3500.446 kb on + strand, within Dsui_3260 at 3500.471 kb on - strand, within Dsui_3260 at 3500.660 kb on - strand, within Dsui_3260 at 3500.701 kb on - strand, within Dsui_3260 at 3500.749 kb on - strand, within Dsui_3260 at 3500.793 kb on + strand, within Dsui_3260 at 3500.793 kb on + strand at 3500.796 kb on - strand, within Dsui_3260 at 3500.804 kb on + strand, within Dsui_3260 at 3500.881 kb on + strand, within Dsui_3260 at 3500.965 kb on - strand at 3501.000 kb on - strand at 3501.002 kb on - strand at 3501.118 kb on - strand at 3501.123 kb on - strand at 3501.128 kb on - strand at 3501.155 kb on - strand at 3501.165 kb on - strand at 3501.174 kb on + strand at 3501.174 kb on + strand at 3501.182 kb on - strand at 3501.183 kb on - strand at 3501.263 kb on + strand at 3501.271 kb on - strand at 3501.279 kb on + strand at 3501.361 kb on - strand at 3501.368 kb on - strand at 3501.472 kb on - strand, within Dsui_3261 at 3501.546 kb on + strand, within Dsui_3261 at 3501.546 kb on + strand, within Dsui_3261 at 3501.567 kb on + strand, within Dsui_3261 at 3501.575 kb on - strand, within Dsui_3261 at 3501.579 kb on + strand, within Dsui_3261 at 3501.585 kb on - strand, within Dsui_3261 at 3501.587 kb on - strand, within Dsui_3261 at 3501.602 kb on - strand, within Dsui_3261 at 3501.656 kb on - strand, within Dsui_3261 at 3501.776 kb on + strand, within Dsui_3261 at 3501.777 kb on - strand, within Dsui_3261 at 3501.808 kb on + strand at 3501.830 kb on - strand, within Dsui_3261 at 3502.015 kb on + strand, within Dsui_3261 at 3502.016 kb on + strand, within Dsui_3261
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,498,106 - +0.9 3,498,145 + -2.3 3,498,247 + -0.1 3,498,332 + Dsui_3259 0.11 +1.6 3,498,517 + Dsui_3259 0.35 +0.2 3,498,522 - -0.1 3,498,552 - -1.0 3,498,696 - Dsui_3259 0.59 -1.7 3,498,744 + Dsui_3259 0.65 -0.3 3,498,764 + Dsui_3259 0.67 -0.3 3,498,770 + Dsui_3259 0.68 +1.0 3,498,770 + Dsui_3259 0.68 -0.4 3,498,778 - -2.6 3,498,920 + Dsui_3259 0.88 -0.2 3,498,940 + +0.0 3,498,941 - +0.3 3,499,077 + +0.3 3,499,078 + -0.4 3,499,168 - -1.2 3,499,205 + -1.5 3,499,306 + -2.3 3,499,355 - Dsui_3260 0.13 -1.0 3,499,363 + -2.7 3,499,531 - Dsui_3260 0.22 -1.2 3,499,588 + Dsui_3260 0.25 -1.0 3,499,663 - Dsui_3260 0.28 -0.3 3,499,702 - Dsui_3260 0.30 -0.8 3,499,734 - Dsui_3260 0.32 -0.6 3,499,752 - Dsui_3260 0.32 -0.8 3,499,778 + -0.6 3,499,814 + -1.0 3,499,847 + -3.0 3,499,889 + Dsui_3260 0.39 -0.8 3,499,993 + +0.6 3,500,070 + Dsui_3260 0.48 -1.7 3,500,078 - Dsui_3260 0.48 -0.7 3,500,125 - Dsui_3260 0.50 -0.6 3,500,125 - Dsui_3260 0.50 -0.6 3,500,125 - Dsui_3260 0.50 -0.4 3,500,196 - Dsui_3260 0.54 -0.4 3,500,446 + Dsui_3260 0.66 -3.1 3,500,471 - Dsui_3260 0.67 -0.8 3,500,660 - Dsui_3260 0.76 -1.1 3,500,701 - Dsui_3260 0.78 -0.3 3,500,749 - Dsui_3260 0.81 -0.1 3,500,793 + Dsui_3260 0.83 +1.3 3,500,793 + -0.6 3,500,796 - Dsui_3260 0.83 -1.1 3,500,804 + Dsui_3260 0.83 -1.4 3,500,881 + Dsui_3260 0.87 +0.0 3,500,965 - -0.3 3,501,000 - -0.2 3,501,002 - -1.7 3,501,118 - -0.3 3,501,123 - -0.4 3,501,128 - -0.5 3,501,155 - -0.4 3,501,165 - +0.4 3,501,174 + -0.2 3,501,174 + -1.1 3,501,182 - +0.7 3,501,183 - -0.1 3,501,263 + -1.6 3,501,271 - +1.0 3,501,279 + -0.8 3,501,361 - +1.1 3,501,368 - +0.7 3,501,472 - Dsui_3261 0.16 +0.7 3,501,546 + Dsui_3261 0.22 -2.1 3,501,546 + Dsui_3261 0.22 -0.2 3,501,567 + Dsui_3261 0.24 -0.3 3,501,575 - Dsui_3261 0.24 +1.2 3,501,579 + Dsui_3261 0.25 +0.0 3,501,585 - Dsui_3261 0.25 +0.6 3,501,587 - Dsui_3261 0.25 +1.1 3,501,602 - Dsui_3261 0.26 +0.7 3,501,656 - Dsui_3261 0.31 +0.2 3,501,776 + Dsui_3261 0.40 -0.3 3,501,777 - Dsui_3261 0.40 +0.4 3,501,808 + -2.2 3,501,830 - Dsui_3261 0.44 +0.8 3,502,015 + Dsui_3261 0.59 -0.6 3,502,016 + Dsui_3261 0.59 -0.1
Or see this region's nucleotide sequence