Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2698 and Dsui_2699 are separated by 51 nucleotides Dsui_2699 and Dsui_2700 are separated by 13 nucleotides Dsui_2700 and Dsui_2701 overlap by 17 nucleotides
Dsui_2698: Dsui_2698 - purine nucleoside phosphorylase, at 2,850,894 to 2,851,646
_2698
Dsui_2699: Dsui_2699 - hypoxanthine-guanine phosphoribosyltransferase, at 2,851,698 to 2,852,246
_2699
Dsui_2700: Dsui_2700 - UTP-glucose-1-phosphate uridylyltransferase, at 2,852,260 to 2,853,126
_2700
Dsui_2701: Dsui_2701 - DNA ligase, NAD-dependent, at 2,853,110 to 2,855,500
_2701
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2851.263 kb on + strand at 2851.517 kb on - strand, within Dsui_2698 at 2851.547 kb on + strand, within Dsui_2698 at 2851.614 kb on + strand at 2851.621 kb on - strand at 2851.622 kb on - strand at 2851.864 kb on + strand at 2851.864 kb on + strand, within Dsui_2699 at 2851.963 kb on - strand, within Dsui_2699 at 2851.973 kb on - strand, within Dsui_2699 at 2851.981 kb on + strand at 2851.981 kb on + strand at 2852.010 kb on - strand, within Dsui_2699 at 2852.120 kb on - strand, within Dsui_2699 at 2852.129 kb on + strand, within Dsui_2699 at 2852.242 kb on + strand at 2852.242 kb on + strand at 2852.242 kb on + strand at 2852.250 kb on - strand at 2852.250 kb on - strand at 2852.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,851,263 + -3.0 2,851,517 - Dsui_2698 0.83 -2.1 2,851,547 + Dsui_2698 0.87 -2.9 2,851,614 + -2.4 2,851,621 - -1.1 2,851,622 - -3.5 2,851,864 + -2.0 2,851,864 + Dsui_2699 0.30 -4.3 2,851,963 - Dsui_2699 0.48 +1.5 2,851,973 - Dsui_2699 0.50 +1.8 2,851,981 + -1.3 2,851,981 + -2.3 2,852,010 - Dsui_2699 0.57 +1.6 2,852,120 - Dsui_2699 0.77 +1.7 2,852,129 + Dsui_2699 0.79 +0.9 2,852,242 + -0.9 2,852,242 + +1.3 2,852,242 + -1.7 2,852,250 - +1.4 2,852,250 - +1.1 2,852,250 - +1.9
Or see this region's nucleotide sequence