Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2652 and Dsui_2653 are separated by 29 nucleotides Dsui_2653 and Dsui_2654 are separated by 16 nucleotides Dsui_2654 and Dsui_2655 overlap by 4 nucleotides Dsui_2655 and Dsui_2656 are separated by 13 nucleotides
Dsui_2652: Dsui_2652 - hydrolase, TatD family, at 2,808,188 to 2,808,970
_2652
Dsui_2653: Dsui_2653 - Tfp pilus assembly protein PilZ, at 2,809,000 to 2,809,386
_2653
Dsui_2654: Dsui_2654 - DNA polymerase III, delta'' subunit, at 2,809,403 to 2,810,428
_2654
Dsui_2655: Dsui_2655 - thymidylate kinase, at 2,810,425 to 2,811,036
_2655
Dsui_2656: Dsui_2656 - conserved hypothetical protein, YceG family, at 2,811,050 to 2,812,045
_2656
Position (kb)
2809
2810
2811 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2808.493 kb on + strand at 2808.513 kb on - strand, within Dsui_2652 at 2808.527 kb on + strand, within Dsui_2652 at 2808.580 kb on + strand at 2808.580 kb on + strand at 2808.617 kb on - strand, within Dsui_2652 at 2808.622 kb on - strand, within Dsui_2652 at 2808.624 kb on + strand, within Dsui_2652 at 2808.765 kb on + strand, within Dsui_2652 at 2808.773 kb on - strand, within Dsui_2652 at 2808.795 kb on - strand, within Dsui_2652 at 2808.815 kb on + strand at 2808.816 kb on + strand at 2808.835 kb on - strand, within Dsui_2652 at 2808.855 kb on - strand, within Dsui_2652 at 2808.941 kb on + strand at 2809.037 kb on - strand at 2809.063 kb on - strand, within Dsui_2653 at 2809.108 kb on + strand, within Dsui_2653 at 2809.123 kb on - strand, within Dsui_2653 at 2809.131 kb on - strand, within Dsui_2653 at 2809.188 kb on + strand at 2809.196 kb on - strand, within Dsui_2653 at 2809.390 kb on - strand at 2809.409 kb on - strand at 2811.094 kb on + strand at 2811.250 kb on + strand at 2811.250 kb on - strand, within Dsui_2656 at 2811.265 kb on + strand, within Dsui_2656 at 2811.273 kb on - strand, within Dsui_2656 at 2811.327 kb on + strand, within Dsui_2656
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,808,493 + -2.1 2,808,513 - Dsui_2652 0.42 +1.1 2,808,527 + Dsui_2652 0.43 +0.1 2,808,580 + -0.5 2,808,580 + -0.1 2,808,617 - Dsui_2652 0.55 +1.3 2,808,622 - Dsui_2652 0.55 +1.9 2,808,624 + Dsui_2652 0.56 -2.1 2,808,765 + Dsui_2652 0.74 -0.0 2,808,773 - Dsui_2652 0.75 +4.9 2,808,795 - Dsui_2652 0.78 +2.4 2,808,815 + +0.6 2,808,816 + -3.7 2,808,835 - Dsui_2652 0.83 +0.6 2,808,855 - Dsui_2652 0.85 +0.8 2,808,941 + -1.9 2,809,037 - +1.4 2,809,063 - Dsui_2653 0.16 +1.5 2,809,108 + Dsui_2653 0.28 +1.2 2,809,123 - Dsui_2653 0.32 +1.8 2,809,131 - Dsui_2653 0.34 +2.1 2,809,188 + -2.4 2,809,196 - Dsui_2653 0.51 +1.5 2,809,390 - +1.8 2,809,409 - +1.1 2,811,094 + +4.4 2,811,250 + -0.2 2,811,250 - Dsui_2656 0.20 +1.3 2,811,265 + Dsui_2656 0.22 -2.7 2,811,273 - Dsui_2656 0.22 +0.7 2,811,327 + Dsui_2656 0.28 -2.6
Or see this region's nucleotide sequence