Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1233 and Dsui_1234 are separated by 134 nucleotides Dsui_1234 and Dsui_1235 are separated by 77 nucleotides Dsui_1235 and Dsui_1236 overlap by 4 nucleotides
Dsui_1233: Dsui_1233 - TRAP-type mannitol/chloroaromatic compound transport system, periplasmic component, at 1,291,035 to 1,292,126
_1233
Dsui_1234: Dsui_1234 - putative Zn-dependent protease-like protein, at 1,292,261 to 1,293,655
_1234
Dsui_1235: Dsui_1235 - hypothetical protein, at 1,293,733 to 1,294,290
_1235
Dsui_1236: Dsui_1236 - molybdenum cofactor synthesis domain protein, at 1,294,287 to 1,294,880
_1236
Position (kb)
1292
1293
1294 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1291.373 kb on + strand, within Dsui_1233 at 1291.381 kb on - strand, within Dsui_1233 at 1291.381 kb on - strand, within Dsui_1233 at 1291.386 kb on + strand, within Dsui_1233 at 1291.441 kb on - strand, within Dsui_1233 at 1291.510 kb on + strand, within Dsui_1233 at 1291.704 kb on + strand, within Dsui_1233 at 1291.716 kb on + strand, within Dsui_1233 at 1291.775 kb on + strand, within Dsui_1233 at 1291.793 kb on - strand, within Dsui_1233 at 1291.797 kb on + strand at 1291.798 kb on + strand, within Dsui_1233 at 1291.806 kb on - strand, within Dsui_1233 at 1291.806 kb on - strand, within Dsui_1233 at 1291.953 kb on + strand, within Dsui_1233 at 1291.977 kb on - strand, within Dsui_1233 at 1291.980 kb on - strand, within Dsui_1233 at 1291.981 kb on - strand, within Dsui_1233 at 1291.983 kb on + strand, within Dsui_1233 at 1292.134 kb on + strand at 1292.221 kb on - strand at 1292.223 kb on + strand at 1292.308 kb on + strand at 1292.308 kb on + strand at 1292.316 kb on - strand at 1292.316 kb on - strand at 1292.316 kb on - strand at 1292.316 kb on - strand at 1292.337 kb on - strand at 1292.364 kb on + strand at 1292.450 kb on - strand, within Dsui_1234 at 1292.451 kb on - strand, within Dsui_1234 at 1292.453 kb on + strand, within Dsui_1234 at 1292.476 kb on - strand, within Dsui_1234 at 1292.603 kb on - strand, within Dsui_1234 at 1292.624 kb on - strand, within Dsui_1234 at 1292.657 kb on - strand, within Dsui_1234 at 1292.723 kb on - strand, within Dsui_1234 at 1292.723 kb on - strand, within Dsui_1234 at 1292.959 kb on - strand, within Dsui_1234 at 1293.077 kb on - strand, within Dsui_1234 at 1293.179 kb on + strand, within Dsui_1234 at 1293.187 kb on - strand, within Dsui_1234 at 1293.337 kb on - strand, within Dsui_1234 at 1293.337 kb on - strand, within Dsui_1234 at 1293.356 kb on - strand, within Dsui_1234 at 1293.403 kb on + strand, within Dsui_1234 at 1293.415 kb on - strand, within Dsui_1234 at 1293.415 kb on - strand, within Dsui_1234 at 1293.445 kb on + strand, within Dsui_1234 at 1293.488 kb on + strand at 1293.666 kb on + strand at 1293.681 kb on - strand at 1293.701 kb on + strand at 1293.703 kb on + strand at 1293.709 kb on - strand at 1293.711 kb on - strand at 1293.711 kb on - strand at 1293.721 kb on - strand at 1293.721 kb on - strand at 1294.011 kb on + strand, within Dsui_1235 at 1294.109 kb on + strand, within Dsui_1235 at 1294.119 kb on - strand, within Dsui_1235 at 1294.166 kb on + strand, within Dsui_1235 at 1294.188 kb on - strand at 1294.325 kb on - strand at 1294.393 kb on + strand, within Dsui_1236 at 1294.451 kb on + strand, within Dsui_1236 at 1294.554 kb on + strand, within Dsui_1236 at 1294.567 kb on - strand, within Dsui_1236 at 1294.648 kb on + strand, within Dsui_1236
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,291,373 + Dsui_1233 0.31 +1.0 1,291,381 - Dsui_1233 0.32 +1.7 1,291,381 - Dsui_1233 0.32 +1.5 1,291,386 + Dsui_1233 0.32 -0.1 1,291,441 - Dsui_1233 0.37 +2.0 1,291,510 + Dsui_1233 0.43 +1.3 1,291,704 + Dsui_1233 0.61 +0.6 1,291,716 + Dsui_1233 0.62 +0.3 1,291,775 + Dsui_1233 0.68 +0.1 1,291,793 - Dsui_1233 0.69 +1.2 1,291,797 + +0.9 1,291,798 + Dsui_1233 0.70 +0.7 1,291,806 - Dsui_1233 0.71 +1.9 1,291,806 - Dsui_1233 0.71 +1.7 1,291,953 + Dsui_1233 0.84 -0.2 1,291,977 - Dsui_1233 0.86 +2.6 1,291,980 - Dsui_1233 0.87 +2.1 1,291,981 - Dsui_1233 0.87 +0.9 1,291,983 + Dsui_1233 0.87 -0.5 1,292,134 + +1.6 1,292,221 - +0.7 1,292,223 + +0.1 1,292,308 + -1.3 1,292,308 + -0.6 1,292,316 - +1.1 1,292,316 - +0.1 1,292,316 - -0.2 1,292,316 - -0.8 1,292,337 - -0.3 1,292,364 + -0.1 1,292,450 - Dsui_1234 0.14 +0.1 1,292,451 - Dsui_1234 0.14 +0.1 1,292,453 + Dsui_1234 0.14 -1.2 1,292,476 - Dsui_1234 0.15 -0.1 1,292,603 - Dsui_1234 0.25 +0.9 1,292,624 - Dsui_1234 0.26 -0.1 1,292,657 - Dsui_1234 0.28 +0.2 1,292,723 - Dsui_1234 0.33 -0.2 1,292,723 - Dsui_1234 0.33 -0.2 1,292,959 - Dsui_1234 0.50 -0.5 1,293,077 - Dsui_1234 0.58 -0.3 1,293,179 + Dsui_1234 0.66 -0.4 1,293,187 - Dsui_1234 0.66 +0.0 1,293,337 - Dsui_1234 0.77 +0.1 1,293,337 - Dsui_1234 0.77 -0.2 1,293,356 - Dsui_1234 0.78 -1.5 1,293,403 + Dsui_1234 0.82 -4.0 1,293,415 - Dsui_1234 0.83 -0.4 1,293,415 - Dsui_1234 0.83 -0.2 1,293,445 + Dsui_1234 0.85 -1.4 1,293,488 + -0.7 1,293,666 + +0.4 1,293,681 - +0.5 1,293,701 + +1.1 1,293,703 + +0.9 1,293,709 - +0.9 1,293,711 - -0.6 1,293,711 - -1.0 1,293,721 - +1.4 1,293,721 - -2.3 1,294,011 + Dsui_1235 0.50 -0.1 1,294,109 + Dsui_1235 0.67 -2.3 1,294,119 - Dsui_1235 0.69 -2.9 1,294,166 + Dsui_1235 0.78 -4.2 1,294,188 - -0.1 1,294,325 - -4.2 1,294,393 + Dsui_1236 0.18 -5.0 1,294,451 + Dsui_1236 0.28 -5.5 1,294,554 + Dsui_1236 0.45 -5.1 1,294,567 - Dsui_1236 0.47 -4.9 1,294,648 + Dsui_1236 0.61 -4.9
Or see this region's nucleotide sequence