Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1179 and Dsui_1180 are separated by 286 nucleotides Dsui_1180 and Dsui_1181 overlap by 4 nucleotides Dsui_1181 and Dsui_1182 are separated by 72 nucleotides
Dsui_1179: Dsui_1179 - ribosome-associated heat shock protein implicated in recycling of 50S subunit, at 1,232,849 to 1,233,268
_1179
Dsui_1180: Dsui_1180 - response regulator consisting of a CheY-like receiver domain and a Fis-type HTH domain protein, at 1,233,555 to 1,234,136
_1180
Dsui_1181: Dsui_1181 - signal transduction histidine kinase, at 1,234,133 to 1,235,533
_1181
Dsui_1182: Dsui_1182 - diaminopimelate decarboxylase, at 1,235,606 to 1,236,862
_1182
Position (kb) 1234
1235
1236 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1233.242 kb on + strand at 1233.257 kb on + strand at 1233.292 kb on - strand at 1233.396 kb on + strand at 1233.533 kb on + strand at 1233.593 kb on + strand at 1233.598 kb on - strand at 1233.684 kb on - strand, within Dsui_1180 at 1233.754 kb on - strand, within Dsui_1180 at 1233.791 kb on + strand, within Dsui_1180 at 1233.899 kb on - strand, within Dsui_1180 at 1233.917 kb on - strand, within Dsui_1180 at 1233.927 kb on + strand, within Dsui_1180 at 1233.960 kb on + strand, within Dsui_1180 at 1233.968 kb on - strand, within Dsui_1180 at 1233.968 kb on - strand, within Dsui_1180 at 1234.067 kb on + strand at 1234.091 kb on - strand at 1234.124 kb on - strand at 1234.209 kb on + strand at 1234.219 kb on + strand at 1234.240 kb on + strand at 1234.240 kb on + strand at 1234.248 kb on - strand at 1234.349 kb on + strand, within Dsui_1181 at 1234.396 kb on - strand, within Dsui_1181 at 1234.396 kb on - strand, within Dsui_1181 at 1234.403 kb on - strand, within Dsui_1181 at 1234.479 kb on - strand, within Dsui_1181 at 1234.500 kb on + strand, within Dsui_1181 at 1234.648 kb on + strand, within Dsui_1181 at 1234.683 kb on - strand, within Dsui_1181 at 1234.683 kb on - strand, within Dsui_1181 at 1234.683 kb on - strand, within Dsui_1181 at 1234.684 kb on - strand, within Dsui_1181 at 1234.714 kb on + strand, within Dsui_1181 at 1234.714 kb on + strand, within Dsui_1181 at 1234.729 kb on - strand, within Dsui_1181 at 1234.759 kb on + strand, within Dsui_1181 at 1234.825 kb on + strand, within Dsui_1181 at 1234.845 kb on + strand, within Dsui_1181 at 1234.847 kb on + strand, within Dsui_1181 at 1234.860 kb on + strand at 1234.894 kb on + strand, within Dsui_1181 at 1234.989 kb on - strand, within Dsui_1181 at 1235.245 kb on + strand, within Dsui_1181 at 1235.303 kb on - strand, within Dsui_1181 at 1235.321 kb on + strand, within Dsui_1181 at 1235.352 kb on - strand, within Dsui_1181 at 1235.409 kb on + strand at 1235.416 kb on - strand at 1235.604 kb on + strand at 1235.604 kb on + strand at 1235.604 kb on + strand at 1235.604 kb on + strand at 1235.604 kb on + strand at 1235.611 kb on + strand at 1235.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,233,242 + +0.5 1,233,257 + +0.3 1,233,292 - +0.6 1,233,396 + +0.1 1,233,533 + +0.4 1,233,593 + -3.6 1,233,598 - -0.3 1,233,684 - Dsui_1180 0.22 -0.1 1,233,754 - Dsui_1180 0.34 -1.8 1,233,791 + Dsui_1180 0.41 -0.2 1,233,899 - Dsui_1180 0.59 -1.3 1,233,917 - Dsui_1180 0.62 -0.9 1,233,927 + Dsui_1180 0.64 -0.9 1,233,960 + Dsui_1180 0.70 -0.9 1,233,968 - Dsui_1180 0.71 +0.1 1,233,968 - Dsui_1180 0.71 +1.4 1,234,067 + -4.1 1,234,091 - -0.5 1,234,124 - +0.4 1,234,209 + -0.3 1,234,219 + +0.1 1,234,240 + +0.2 1,234,240 + +0.3 1,234,248 - +0.8 1,234,349 + Dsui_1181 0.15 +1.2 1,234,396 - Dsui_1181 0.19 +0.3 1,234,396 - Dsui_1181 0.19 +1.0 1,234,403 - Dsui_1181 0.19 +0.3 1,234,479 - Dsui_1181 0.25 +0.9 1,234,500 + Dsui_1181 0.26 +0.2 1,234,648 + Dsui_1181 0.37 +1.7 1,234,683 - Dsui_1181 0.39 +1.0 1,234,683 - Dsui_1181 0.39 +1.7 1,234,683 - Dsui_1181 0.39 +1.1 1,234,684 - Dsui_1181 0.39 +0.3 1,234,714 + Dsui_1181 0.41 -1.5 1,234,714 + Dsui_1181 0.41 -0.6 1,234,729 - Dsui_1181 0.43 -0.3 1,234,759 + Dsui_1181 0.45 -0.2 1,234,825 + Dsui_1181 0.49 -1.7 1,234,845 + Dsui_1181 0.51 -1.1 1,234,847 + Dsui_1181 0.51 -0.5 1,234,860 + +0.2 1,234,894 + Dsui_1181 0.54 -1.7 1,234,989 - Dsui_1181 0.61 -0.4 1,235,245 + Dsui_1181 0.79 -0.5 1,235,303 - Dsui_1181 0.84 +1.1 1,235,321 + Dsui_1181 0.85 +1.0 1,235,352 - Dsui_1181 0.87 +0.7 1,235,409 + -0.0 1,235,416 - +0.6 1,235,604 + +0.3 1,235,604 + +0.6 1,235,604 + +2.0 1,235,604 + +0.7 1,235,604 + -0.1 1,235,611 + -0.2 1,235,612 - +1.0
Or see this region's nucleotide sequence