Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0665 and Dsui_0666 are separated by 145 nucleotides Dsui_0666 and Dsui_0667 are separated by 305 nucleotides Dsui_0667 and Dsui_0668 are separated by 186 nucleotides
Dsui_0665: Dsui_0665 - polyphosphate kinase 2, PA0141 family, at 662,089 to 663,288
_0665
Dsui_0666: Dsui_0666 - Na+/proline symporter, at 663,434 to 664,936
_0666
Dsui_0667: Dsui_0667 - hypothetical protein, at 665,242 to 665,448
_0667
Dsui_0668: Dsui_0668 - putative membrane protein, at 665,635 to 668,355
_0668
Position (kb)
663
664
665 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 662.454 kb on + strand, within Dsui_0665 at 662.454 kb on + strand, within Dsui_0665 at 662.454 kb on + strand, within Dsui_0665 at 662.568 kb on + strand, within Dsui_0665 at 662.668 kb on - strand, within Dsui_0665 at 662.708 kb on + strand, within Dsui_0665 at 662.716 kb on - strand, within Dsui_0665 at 662.751 kb on - strand, within Dsui_0665 at 662.792 kb on - strand, within Dsui_0665 at 663.037 kb on - strand, within Dsui_0665 at 663.048 kb on + strand, within Dsui_0665 at 663.048 kb on + strand, within Dsui_0665 at 663.056 kb on - strand, within Dsui_0665 at 663.081 kb on + strand, within Dsui_0665 at 663.135 kb on + strand, within Dsui_0665 at 663.161 kb on - strand, within Dsui_0665 at 663.197 kb on + strand at 663.620 kb on + strand at 663.686 kb on - strand, within Dsui_0666 at 663.941 kb on + strand, within Dsui_0666 at 663.959 kb on - strand, within Dsui_0666 at 664.037 kb on - strand, within Dsui_0666 at 664.109 kb on + strand, within Dsui_0666 at 664.217 kb on - strand, within Dsui_0666 at 664.219 kb on + strand, within Dsui_0666 at 664.400 kb on + strand, within Dsui_0666 at 664.408 kb on - strand, within Dsui_0666 at 664.527 kb on + strand, within Dsui_0666 at 664.527 kb on + strand at 664.535 kb on - strand, within Dsui_0666 at 664.535 kb on - strand, within Dsui_0666 at 664.612 kb on + strand, within Dsui_0666 at 664.724 kb on + strand, within Dsui_0666 at 664.732 kb on - strand, within Dsui_0666 at 664.919 kb on + strand at 664.919 kb on + strand at 664.937 kb on + strand at 664.984 kb on + strand at 664.984 kb on + strand at 664.989 kb on + strand at 664.989 kb on + strand at 664.992 kb on - strand at 665.027 kb on - strand at 665.086 kb on + strand at 665.086 kb on + strand at 665.086 kb on + strand at 665.087 kb on + strand at 665.087 kb on + strand at 665.092 kb on + strand at 665.094 kb on - strand at 665.094 kb on - strand at 665.094 kb on - strand at 665.095 kb on - strand at 665.095 kb on - strand at 665.097 kb on + strand at 665.097 kb on + strand at 665.097 kb on + strand at 665.100 kb on - strand at 665.105 kb on - strand at 665.105 kb on - strand at 665.105 kb on - strand at 665.110 kb on + strand at 665.118 kb on - strand at 665.134 kb on + strand at 665.134 kb on + strand at 665.142 kb on - strand at 665.148 kb on - strand at 665.266 kb on + strand, within Dsui_0667 at 665.266 kb on + strand at 665.266 kb on + strand, within Dsui_0667 at 665.266 kb on + strand, within Dsui_0667 at 665.313 kb on + strand at 665.313 kb on + strand, within Dsui_0667 at 665.313 kb on + strand, within Dsui_0667 at 665.330 kb on + strand, within Dsui_0667 at 665.333 kb on - strand, within Dsui_0667 at 665.371 kb on - strand, within Dsui_0667 at 665.446 kb on + strand at 665.504 kb on - strand at 665.530 kb on - strand at 665.536 kb on - strand at 665.685 kb on - strand at 665.685 kb on - strand at 665.703 kb on - strand at 665.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 662,454 + Dsui_0665 0.30 +0.2 662,454 + Dsui_0665 0.30 +0.9 662,454 + Dsui_0665 0.30 +0.9 662,568 + Dsui_0665 0.40 -0.8 662,668 - Dsui_0665 0.48 +1.1 662,708 + Dsui_0665 0.52 +1.7 662,716 - Dsui_0665 0.52 +1.1 662,751 - Dsui_0665 0.55 +0.7 662,792 - Dsui_0665 0.59 +1.4 663,037 - Dsui_0665 0.79 +0.8 663,048 + Dsui_0665 0.80 +2.0 663,048 + Dsui_0665 0.80 +1.0 663,056 - Dsui_0665 0.81 -0.4 663,081 + Dsui_0665 0.83 -4.0 663,135 + Dsui_0665 0.87 +0.4 663,161 - Dsui_0665 0.89 +0.5 663,197 + +1.6 663,620 + -2.6 663,686 - Dsui_0666 0.17 -0.5 663,941 + Dsui_0666 0.34 -2.5 663,959 - Dsui_0666 0.35 -0.3 664,037 - Dsui_0666 0.40 -1.1 664,109 + Dsui_0666 0.45 -1.2 664,217 - Dsui_0666 0.52 -0.2 664,219 + Dsui_0666 0.52 -0.6 664,400 + Dsui_0666 0.64 -0.5 664,408 - Dsui_0666 0.65 -0.6 664,527 + Dsui_0666 0.73 -2.8 664,527 + -1.5 664,535 - Dsui_0666 0.73 -2.0 664,535 - Dsui_0666 0.73 -2.1 664,612 + Dsui_0666 0.78 -0.9 664,724 + Dsui_0666 0.86 -1.7 664,732 - Dsui_0666 0.86 -1.2 664,919 + -2.8 664,919 + -0.2 664,937 + -1.9 664,984 + -0.9 664,984 + -0.9 664,989 + -1.2 664,989 + -1.9 664,992 - +0.6 665,027 - +0.4 665,086 + -2.1 665,086 + -0.2 665,086 + -1.9 665,087 + +0.8 665,087 + -1.1 665,092 + +1.4 665,094 - -0.3 665,094 - -0.3 665,094 - -0.7 665,095 - -1.0 665,095 - +0.6 665,097 + -0.6 665,097 + -0.1 665,097 + -0.4 665,100 - +0.3 665,105 - -0.6 665,105 - -0.5 665,105 - +1.6 665,110 + -2.9 665,118 - +0.3 665,134 + -0.4 665,134 + -0.2 665,142 - -1.7 665,148 - +0.3 665,266 + Dsui_0667 0.12 -1.2 665,266 + +0.0 665,266 + Dsui_0667 0.12 +0.1 665,266 + Dsui_0667 0.12 +0.2 665,313 + +1.3 665,313 + Dsui_0667 0.34 -0.7 665,313 + Dsui_0667 0.34 -1.1 665,330 + Dsui_0667 0.43 -0.5 665,333 - Dsui_0667 0.44 -1.8 665,371 - Dsui_0667 0.62 -0.7 665,446 + +0.2 665,504 - -0.4 665,530 - +0.8 665,536 - +1.1 665,685 - -0.2 665,685 - -0.6 665,703 - -0.6 665,822 - +0.4
Or see this region's nucleotide sequence