Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0601 and Dsui_0602 are separated by 39 nucleotides Dsui_0602 and Dsui_0603 are separated by 13 nucleotides Dsui_0603 and Dsui_0604 are separated by 158 nucleotides
Dsui_0601: Dsui_0601 - putative integral membrane protein, at 596,677 to 597,249
_0601
Dsui_0602: Dsui_0602 - pyrroline-5-carboxylate reductase, at 597,289 to 598,101
_0602
Dsui_0603: Dsui_0603 - pyridoxal phosphate enzyme, YggS family, at 598,115 to 598,819
_0603
Dsui_0604: Dsui_0604 - pilus retraction protein PilT, at 598,978 to 600,021
_0604
Position (kb)
598
599 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 597.140 kb on - strand, within Dsui_0601 at 597.274 kb on - strand at 597.280 kb on - strand at 597.310 kb on + strand at 598.160 kb on - strand at 598.176 kb on + strand at 598.200 kb on + strand, within Dsui_0603 at 598.212 kb on + strand, within Dsui_0603 at 598.262 kb on + strand, within Dsui_0603 at 598.355 kb on - strand, within Dsui_0603 at 598.409 kb on + strand at 598.451 kb on - strand, within Dsui_0603 at 598.564 kb on + strand at 598.710 kb on + strand, within Dsui_0603 at 598.712 kb on - strand, within Dsui_0603 at 598.744 kb on + strand, within Dsui_0603 at 598.781 kb on + strand at 598.917 kb on - strand at 598.938 kb on - strand at 598.962 kb on + strand at 599.128 kb on + strand, within Dsui_0604 at 599.128 kb on + strand, within Dsui_0604 at 599.154 kb on + strand, within Dsui_0604 at 599.209 kb on - strand, within Dsui_0604 at 599.291 kb on + strand, within Dsui_0604 at 599.291 kb on + strand, within Dsui_0604 at 599.291 kb on + strand, within Dsui_0604 at 599.291 kb on + strand, within Dsui_0604 at 599.356 kb on - strand, within Dsui_0604 at 599.369 kb on - strand at 599.385 kb on + strand, within Dsui_0604 at 599.387 kb on + strand, within Dsui_0604 at 599.398 kb on - strand, within Dsui_0604 at 599.447 kb on - strand, within Dsui_0604 at 599.447 kb on - strand, within Dsui_0604 at 599.479 kb on + strand, within Dsui_0604 at 599.487 kb on - strand, within Dsui_0604 at 599.487 kb on - strand, within Dsui_0604 at 599.516 kb on + strand, within Dsui_0604 at 599.542 kb on - strand, within Dsui_0604 at 599.544 kb on + strand, within Dsui_0604 at 599.598 kb on + strand, within Dsui_0604 at 599.755 kb on - strand, within Dsui_0604
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 597,140 - Dsui_0601 0.81 +0.2 597,274 - +1.0 597,280 - +1.9 597,310 + -2.4 598,160 - +1.6 598,176 + +0.7 598,200 + Dsui_0603 0.12 -1.0 598,212 + Dsui_0603 0.14 +0.5 598,262 + Dsui_0603 0.21 -0.2 598,355 - Dsui_0603 0.34 +0.6 598,409 + +2.6 598,451 - Dsui_0603 0.48 +0.9 598,564 + +0.0 598,710 + Dsui_0603 0.84 -0.5 598,712 - Dsui_0603 0.85 +0.9 598,744 + Dsui_0603 0.89 +0.3 598,781 + -3.0 598,917 - +0.9 598,938 - -0.2 598,962 + -0.0 599,128 + Dsui_0604 0.14 -4.4 599,128 + Dsui_0604 0.14 -4.8 599,154 + Dsui_0604 0.17 -3.3 599,209 - Dsui_0604 0.22 -2.1 599,291 + Dsui_0604 0.30 -1.7 599,291 + Dsui_0604 0.30 -2.6 599,291 + Dsui_0604 0.30 -4.8 599,291 + Dsui_0604 0.30 -2.8 599,356 - Dsui_0604 0.36 -3.1 599,369 - -3.0 599,385 + Dsui_0604 0.39 -4.3 599,387 + Dsui_0604 0.39 -4.6 599,398 - Dsui_0604 0.40 -4.2 599,447 - Dsui_0604 0.45 -3.7 599,447 - Dsui_0604 0.45 -4.2 599,479 + Dsui_0604 0.48 -2.2 599,487 - Dsui_0604 0.49 -5.2 599,487 - Dsui_0604 0.49 -3.4 599,516 + Dsui_0604 0.52 -2.4 599,542 - Dsui_0604 0.54 -2.7 599,544 + Dsui_0604 0.54 -3.0 599,598 + Dsui_0604 0.59 -3.1 599,755 - Dsui_0604 0.74 -3.6
Or see this region's nucleotide sequence