Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0398 and Dsui_0399 are separated by 2 nucleotides Dsui_0399 and Dsui_0400 are separated by 1 nucleotides Dsui_0400 and Dsui_0401 are separated by 121 nucleotides
Dsui_0398: Dsui_0398 - UDP-glucose-4-epimerase, at 395,875 to 396,888
_0398
Dsui_0399: Dsui_0399 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 396,891 to 397,721
_0399
Dsui_0400: Dsui_0400 - putative nucleoside-diphosphate sugar epimerase, at 397,723 to 399,585
_0400
Dsui_0401: Dsui_0401 - nucleoside-diphosphate-sugar epimerase, at 399,707 to 400,711
_0401
Position (kb) 397
398
399
400 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 396.740 kb on + strand, within Dsui_0398 at 396.740 kb on + strand, within Dsui_0398 at 396.748 kb on - strand, within Dsui_0398 at 396.804 kb on - strand at 396.864 kb on + strand at 396.872 kb on - strand at 397.007 kb on - strand, within Dsui_0399 at 397.141 kb on + strand, within Dsui_0399 at 397.266 kb on - strand, within Dsui_0399 at 397.406 kb on - strand at 397.504 kb on + strand, within Dsui_0399 at 397.538 kb on - strand, within Dsui_0399 at 397.600 kb on + strand at 397.659 kb on + strand at 397.756 kb on - strand at 397.819 kb on + strand at 397.841 kb on + strand at 397.841 kb on + strand at 397.841 kb on + strand at 397.841 kb on + strand at 397.995 kb on - strand, within Dsui_0400 at 398.062 kb on + strand, within Dsui_0400 at 398.253 kb on + strand, within Dsui_0400 at 398.257 kb on - strand, within Dsui_0400 at 398.335 kb on + strand, within Dsui_0400 at 398.358 kb on - strand, within Dsui_0400 at 398.415 kb on + strand, within Dsui_0400 at 398.452 kb on + strand, within Dsui_0400 at 398.455 kb on + strand, within Dsui_0400 at 398.522 kb on + strand, within Dsui_0400 at 398.548 kb on - strand, within Dsui_0400 at 398.662 kb on - strand, within Dsui_0400 at 398.725 kb on + strand, within Dsui_0400 at 398.946 kb on + strand at 398.959 kb on - strand, within Dsui_0400 at 398.985 kb on + strand, within Dsui_0400 at 399.218 kb on - strand, within Dsui_0400 at 399.325 kb on + strand, within Dsui_0400 at 399.333 kb on - strand, within Dsui_0400 at 399.501 kb on + strand at 399.570 kb on + strand at 399.603 kb on - strand at 399.631 kb on + strand at 399.639 kb on - strand at 399.639 kb on - strand at 399.660 kb on + strand at 399.686 kb on - strand at 400.103 kb on + strand at 400.220 kb on - strand, within Dsui_0401 at 400.346 kb on - strand, within Dsui_0401 at 400.492 kb on - strand, within Dsui_0401
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 396,740 + Dsui_0398 0.85 -1.9 396,740 + Dsui_0398 0.85 +0.3 396,748 - Dsui_0398 0.86 -2.0 396,804 - -1.0 396,864 + -1.0 396,872 - -1.6 397,007 - Dsui_0399 0.14 -1.9 397,141 + Dsui_0399 0.30 -3.1 397,266 - Dsui_0399 0.45 -2.2 397,406 - -1.3 397,504 + Dsui_0399 0.74 -2.6 397,538 - Dsui_0399 0.78 -2.0 397,600 + -1.4 397,659 + -2.8 397,756 - -3.5 397,819 + -1.0 397,841 + -2.5 397,841 + -2.4 397,841 + -1.9 397,841 + -2.4 397,995 - Dsui_0400 0.15 -3.8 398,062 + Dsui_0400 0.18 -1.1 398,253 + Dsui_0400 0.28 -2.4 398,257 - Dsui_0400 0.29 -1.0 398,335 + Dsui_0400 0.33 -1.1 398,358 - Dsui_0400 0.34 -0.7 398,415 + Dsui_0400 0.37 -2.3 398,452 + Dsui_0400 0.39 -4.2 398,455 + Dsui_0400 0.39 -2.4 398,522 + Dsui_0400 0.43 -2.4 398,548 - Dsui_0400 0.44 -2.5 398,662 - Dsui_0400 0.50 -1.8 398,725 + Dsui_0400 0.54 -1.7 398,946 + +0.3 398,959 - Dsui_0400 0.66 -1.5 398,985 + Dsui_0400 0.68 -2.9 399,218 - Dsui_0400 0.80 -2.1 399,325 + Dsui_0400 0.86 -3.4 399,333 - Dsui_0400 0.86 +0.9 399,501 + -3.1 399,570 + -1.8 399,603 - -1.0 399,631 + -2.6 399,639 - -0.0 399,639 - +0.7 399,660 + -0.3 399,686 - +0.6 400,103 + -1.0 400,220 - Dsui_0401 0.51 -2.1 400,346 - Dsui_0401 0.64 -1.5 400,492 - Dsui_0401 0.78 -1.0
Or see this region's nucleotide sequence