Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0391 and Dsui_0392 are separated by 96 nucleotides Dsui_0392 and Dsui_0393 are separated by 73 nucleotides Dsui_0393 and Dsui_0394 are separated by 65 nucleotides
Dsui_0391: Dsui_0391 - Protein of unknown function (DUF3426), at 389,887 to 390,879
_0391
Dsui_0392: Dsui_0392 - diacylglycerol kinase, at 390,976 to 391,362
_0392
Dsui_0393: Dsui_0393 - glycosyltransferase, at 391,436 to 392,680
_0393
Dsui_0394: Dsui_0394 - sugar kinase, ribokinase, at 392,746 to 393,681
_0394
Position (kb)
391
392
393 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 390.905 kb on + strand at 390.908 kb on - strand at 390.913 kb on - strand at 390.961 kb on + strand at 390.966 kb on - strand at 391.003 kb on - strand at 391.020 kb on - strand, within Dsui_0392 at 391.030 kb on + strand, within Dsui_0392 at 391.047 kb on + strand, within Dsui_0392 at 391.050 kb on - strand at 391.055 kb on - strand, within Dsui_0392 at 391.133 kb on - strand, within Dsui_0392 at 391.166 kb on + strand, within Dsui_0392 at 391.170 kb on + strand, within Dsui_0392 at 391.179 kb on - strand, within Dsui_0392 at 391.204 kb on + strand at 391.343 kb on + strand at 391.483 kb on - strand at 391.562 kb on + strand at 391.564 kb on - strand, within Dsui_0393 at 391.724 kb on - strand, within Dsui_0393 at 391.748 kb on + strand at 391.778 kb on + strand, within Dsui_0393 at 391.799 kb on + strand, within Dsui_0393 at 391.807 kb on - strand, within Dsui_0393 at 391.903 kb on - strand at 391.903 kb on - strand at 391.913 kb on - strand at 391.938 kb on - strand, within Dsui_0393 at 392.061 kb on - strand, within Dsui_0393 at 392.081 kb on - strand at 392.099 kb on - strand at 392.125 kb on + strand, within Dsui_0393 at 392.194 kb on - strand at 392.237 kb on + strand, within Dsui_0393 at 392.378 kb on - strand at 392.396 kb on - strand at 392.437 kb on + strand at 392.445 kb on - strand, within Dsui_0393 at 392.518 kb on + strand, within Dsui_0393 at 392.680 kb on + strand at 392.705 kb on + strand at 392.748 kb on - strand at 392.765 kb on - strand at 392.821 kb on - strand at 392.912 kb on + strand, within Dsui_0394 at 392.922 kb on + strand, within Dsui_0394 at 393.001 kb on - strand at 393.001 kb on - strand at 393.001 kb on - strand at 393.033 kb on + strand, within Dsui_0394 at 393.041 kb on - strand at 393.051 kb on + strand, within Dsui_0394 at 393.054 kb on + strand, within Dsui_0394 at 393.057 kb on - strand at 393.103 kb on + strand, within Dsui_0394 at 393.110 kb on + strand, within Dsui_0394 at 393.128 kb on - strand, within Dsui_0394 at 393.207 kb on - strand, within Dsui_0394 at 393.252 kb on - strand at 393.277 kb on + strand, within Dsui_0394 at 393.299 kb on - strand, within Dsui_0394 at 393.319 kb on - strand, within Dsui_0394 at 393.423 kb on - strand, within Dsui_0394 at 393.498 kb on - strand at 393.514 kb on + strand, within Dsui_0394 at 393.519 kb on + strand, within Dsui_0394 at 393.519 kb on + strand, within Dsui_0394
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 390,905 + +0.8 390,908 - +2.6 390,913 - -0.1 390,961 + +1.1 390,966 - +0.6 391,003 - +0.1 391,020 - Dsui_0392 0.11 -1.0 391,030 + Dsui_0392 0.14 +0.4 391,047 + Dsui_0392 0.18 -0.5 391,050 - +1.1 391,055 - Dsui_0392 0.20 +0.9 391,133 - Dsui_0392 0.41 -0.1 391,166 + Dsui_0392 0.49 -0.3 391,170 + Dsui_0392 0.50 +0.3 391,179 - Dsui_0392 0.52 -1.5 391,204 + +2.0 391,343 + +0.2 391,483 - -0.6 391,562 + +0.0 391,564 - Dsui_0393 0.10 -0.9 391,724 - Dsui_0393 0.23 -2.2 391,748 + +1.6 391,778 + Dsui_0393 0.27 -0.1 391,799 + Dsui_0393 0.29 +0.2 391,807 - Dsui_0393 0.30 +1.2 391,903 - -1.3 391,903 - -3.5 391,913 - -1.0 391,938 - Dsui_0393 0.40 +0.1 392,061 - Dsui_0393 0.50 -0.4 392,081 - -0.1 392,099 - +0.8 392,125 + Dsui_0393 0.55 -1.6 392,194 - -0.3 392,237 + Dsui_0393 0.64 +0.4 392,378 - -0.4 392,396 - -2.9 392,437 + -1.8 392,445 - Dsui_0393 0.81 -0.3 392,518 + Dsui_0393 0.87 -0.7 392,680 + -1.4 392,705 + +0.6 392,748 - -0.7 392,765 - -1.1 392,821 - -1.7 392,912 + Dsui_0394 0.18 -0.4 392,922 + Dsui_0394 0.19 -1.5 393,001 - -2.5 393,001 - -1.0 393,001 - -0.6 393,033 + Dsui_0394 0.31 +1.3 393,041 - -0.4 393,051 + Dsui_0394 0.33 -0.7 393,054 + Dsui_0394 0.33 -0.4 393,057 - -0.7 393,103 + Dsui_0394 0.38 -0.4 393,110 + Dsui_0394 0.39 +0.5 393,128 - Dsui_0394 0.41 -1.7 393,207 - Dsui_0394 0.49 -0.7 393,252 - +0.7 393,277 + Dsui_0394 0.57 -1.3 393,299 - Dsui_0394 0.59 -0.4 393,319 - Dsui_0394 0.61 -0.8 393,423 - Dsui_0394 0.72 -1.6 393,498 - -1.8 393,514 + Dsui_0394 0.82 -1.3 393,519 + Dsui_0394 0.83 +1.1 393,519 + Dsui_0394 0.83 -1.1
Or see this region's nucleotide sequence