Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17990 and BBR_RS17995 are separated by 374 nucleotides BBR_RS17995 and BBR_RS18000 are separated by 211 nucleotides
BBR_RS17990: BBR_RS17990 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,823,571 to 1,824,590
_RS17990
BBR_RS17995: BBR_RS17995 - citrate synthase, at 1,824,965 to 1,826,257
_RS17995
BBR_RS18000: BBR_RS18000 - type I methionyl aminopeptidase, at 1,826,469 to 1,827,251
_RS18000
Position (kb)
1824
1825
1826
1827 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1823.991 kb on + strand, within BBR_RS17990 at 1824.035 kb on - strand, within BBR_RS17990 at 1824.047 kb on + strand, within BBR_RS17990 at 1824.061 kb on - strand, within BBR_RS17990 at 1824.078 kb on + strand, within BBR_RS17990 at 1824.119 kb on + strand, within BBR_RS17990 at 1824.124 kb on + strand, within BBR_RS17990 at 1824.127 kb on - strand, within BBR_RS17990 at 1824.127 kb on - strand, within BBR_RS17990 at 1824.132 kb on - strand, within BBR_RS17990 at 1824.144 kb on - strand, within BBR_RS17990 at 1824.189 kb on + strand, within BBR_RS17990 at 1824.189 kb on + strand, within BBR_RS17990 at 1824.195 kb on - strand, within BBR_RS17990 at 1824.195 kb on - strand, within BBR_RS17990 at 1824.224 kb on + strand, within BBR_RS17990 at 1824.224 kb on + strand, within BBR_RS17990 at 1824.226 kb on + strand, within BBR_RS17990 at 1824.232 kb on - strand, within BBR_RS17990 at 1824.244 kb on + strand, within BBR_RS17990 at 1824.244 kb on + strand, within BBR_RS17990 at 1824.252 kb on - strand, within BBR_RS17990 at 1824.259 kb on - strand, within BBR_RS17990 at 1824.273 kb on + strand, within BBR_RS17990 at 1824.276 kb on - strand, within BBR_RS17990 at 1824.292 kb on + strand, within BBR_RS17990 at 1824.302 kb on + strand, within BBR_RS17990 at 1824.378 kb on + strand, within BBR_RS17990 at 1824.386 kb on - strand, within BBR_RS17990 at 1824.386 kb on - strand, within BBR_RS17990 at 1824.386 kb on - strand, within BBR_RS17990 at 1824.386 kb on - strand, within BBR_RS17990 at 1824.387 kb on + strand, within BBR_RS17990 at 1824.431 kb on + strand, within BBR_RS17990 at 1824.431 kb on + strand, within BBR_RS17990 at 1824.433 kb on - strand, within BBR_RS17990 at 1824.439 kb on - strand, within BBR_RS17990 at 1824.455 kb on + strand, within BBR_RS17990 at 1824.455 kb on + strand, within BBR_RS17990 at 1824.464 kb on + strand, within BBR_RS17990 at 1824.494 kb on + strand at 1824.552 kb on - strand at 1824.584 kb on + strand at 1824.592 kb on - strand at 1824.592 kb on - strand at 1824.592 kb on - strand at 1824.619 kb on - strand at 1824.622 kb on + strand at 1824.658 kb on + strand at 1824.666 kb on + strand at 1824.685 kb on + strand at 1824.715 kb on + strand at 1824.723 kb on - strand at 1824.723 kb on - strand at 1824.735 kb on - strand at 1824.743 kb on - strand at 1824.804 kb on + strand at 1824.838 kb on - strand at 1824.954 kb on + strand at 1824.981 kb on - strand at 1825.006 kb on - strand at 1825.008 kb on + strand at 1825.008 kb on + strand at 1825.008 kb on + strand at 1825.008 kb on + strand at 1825.008 kb on + strand at 1825.008 kb on + strand at 1825.011 kb on - strand at 1825.011 kb on - strand at 1825.016 kb on - strand at 1825.016 kb on - strand at 1825.016 kb on - strand at 1825.055 kb on + strand at 1825.064 kb on + strand at 1825.064 kb on + strand at 1825.064 kb on + strand at 1825.072 kb on - strand at 1825.072 kb on - strand at 1825.072 kb on - strand at 1825.086 kb on + strand at 1825.107 kb on + strand, within BBR_RS17995 at 1825.145 kb on + strand, within BBR_RS17995 at 1825.160 kb on - strand, within BBR_RS17995 at 1825.180 kb on + strand, within BBR_RS17995 at 1825.197 kb on - strand, within BBR_RS17995 at 1825.204 kb on - strand, within BBR_RS17995 at 1825.208 kb on - strand, within BBR_RS17995 at 1825.257 kb on + strand, within BBR_RS17995 at 1825.338 kb on - strand, within BBR_RS17995 at 1825.354 kb on + strand, within BBR_RS17995 at 1825.354 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.377 kb on + strand, within BBR_RS17995 at 1825.385 kb on - strand, within BBR_RS17995 at 1825.385 kb on - strand, within BBR_RS17995 at 1825.385 kb on - strand, within BBR_RS17995 at 1825.385 kb on - strand, within BBR_RS17995 at 1825.385 kb on - strand, within BBR_RS17995 at 1825.400 kb on - strand, within BBR_RS17995 at 1825.469 kb on + strand, within BBR_RS17995 at 1825.475 kb on - strand, within BBR_RS17995 at 1825.477 kb on - strand, within BBR_RS17995 at 1825.487 kb on - strand, within BBR_RS17995 at 1825.487 kb on - strand, within BBR_RS17995 at 1825.510 kb on + strand, within BBR_RS17995 at 1825.522 kb on + strand, within BBR_RS17995 at 1825.526 kb on + strand, within BBR_RS17995 at 1825.534 kb on - strand, within BBR_RS17995 at 1825.617 kb on + strand, within BBR_RS17995 at 1825.618 kb on - strand, within BBR_RS17995 at 1825.656 kb on + strand, within BBR_RS17995 at 1825.760 kb on + strand, within BBR_RS17995 at 1825.827 kb on + strand, within BBR_RS17995 at 1825.835 kb on - strand, within BBR_RS17995 at 1825.835 kb on - strand, within BBR_RS17995 at 1825.836 kb on - strand, within BBR_RS17995 at 1825.859 kb on + strand, within BBR_RS17995 at 1825.867 kb on - strand, within BBR_RS17995 at 1825.879 kb on - strand, within BBR_RS17995 at 1825.932 kb on + strand, within BBR_RS17995 at 1825.979 kb on - strand, within BBR_RS17995 at 1826.108 kb on + strand, within BBR_RS17995 at 1826.113 kb on + strand, within BBR_RS17995 at 1826.162 kb on - strand at 1826.165 kb on - strand at 1826.181 kb on - strand at 1826.272 kb on - strand at 1826.272 kb on - strand at 1826.281 kb on - strand at 1826.326 kb on + strand at 1826.332 kb on - strand at 1826.462 kb on + strand at 1826.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 1,823,991 + BBR_RS17990 0.41 +0.1 1,824,035 - BBR_RS17990 0.45 -1.2 1,824,047 + BBR_RS17990 0.47 -1.4 1,824,061 - BBR_RS17990 0.48 -0.4 1,824,078 + BBR_RS17990 0.50 -1.8 1,824,119 + BBR_RS17990 0.54 -2.0 1,824,124 + BBR_RS17990 0.54 +0.4 1,824,127 - BBR_RS17990 0.55 +0.1 1,824,127 - BBR_RS17990 0.55 +0.6 1,824,132 - BBR_RS17990 0.55 +1.5 1,824,144 - BBR_RS17990 0.56 -0.1 1,824,189 + BBR_RS17990 0.61 -0.6 1,824,189 + BBR_RS17990 0.61 -0.9 1,824,195 - BBR_RS17990 0.61 -0.9 1,824,195 - BBR_RS17990 0.61 +0.6 1,824,224 + BBR_RS17990 0.64 +2.3 1,824,224 + BBR_RS17990 0.64 +2.6 1,824,226 + BBR_RS17990 0.64 +1.3 1,824,232 - BBR_RS17990 0.65 -1.0 1,824,244 + BBR_RS17990 0.66 +0.3 1,824,244 + BBR_RS17990 0.66 +0.9 1,824,252 - BBR_RS17990 0.67 +1.3 1,824,259 - BBR_RS17990 0.67 -0.9 1,824,273 + BBR_RS17990 0.69 -0.2 1,824,276 - BBR_RS17990 0.69 +1.3 1,824,292 + BBR_RS17990 0.71 +1.7 1,824,302 + BBR_RS17990 0.72 +1.4 1,824,378 + BBR_RS17990 0.79 -0.1 1,824,386 - BBR_RS17990 0.80 -0.3 1,824,386 - BBR_RS17990 0.80 +2.9 1,824,386 - BBR_RS17990 0.80 +0.9 1,824,386 - BBR_RS17990 0.80 +1.1 1,824,387 + BBR_RS17990 0.80 +0.1 1,824,431 + BBR_RS17990 0.84 +0.8 1,824,431 + BBR_RS17990 0.84 -1.6 1,824,433 - BBR_RS17990 0.85 +2.7 1,824,439 - BBR_RS17990 0.85 +1.3 1,824,455 + BBR_RS17990 0.87 -0.6 1,824,455 + BBR_RS17990 0.87 +1.9 1,824,464 + BBR_RS17990 0.88 +3.0 1,824,494 + +0.3 1,824,552 - -0.6 1,824,584 + +1.4 1,824,592 - +1.7 1,824,592 - -1.0 1,824,592 - -0.6 1,824,619 - -0.9 1,824,622 + +1.2 1,824,658 + -0.6 1,824,666 + +0.1 1,824,685 + +0.0 1,824,715 + +0.2 1,824,723 - -0.9 1,824,723 - -0.6 1,824,735 - +1.8 1,824,743 - -1.3 1,824,804 + -1.3 1,824,838 - -3.2 1,824,954 + +0.5 1,824,981 - +1.3 1,825,006 - +1.0 1,825,008 + +0.3 1,825,008 + +0.5 1,825,008 + -0.9 1,825,008 + -0.2 1,825,008 + +2.0 1,825,008 + +1.5 1,825,011 - +1.9 1,825,011 - +1.3 1,825,016 - +0.2 1,825,016 - +0.1 1,825,016 - +0.0 1,825,055 + +0.8 1,825,064 + -0.1 1,825,064 + -0.6 1,825,064 + +0.4 1,825,072 - +0.4 1,825,072 - -0.6 1,825,072 - -0.5 1,825,086 + -0.1 1,825,107 + BBR_RS17995 0.11 -0.4 1,825,145 + BBR_RS17995 0.14 -0.8 1,825,160 - BBR_RS17995 0.15 +0.6 1,825,180 + BBR_RS17995 0.17 -1.3 1,825,197 - BBR_RS17995 0.18 +1.1 1,825,204 - BBR_RS17995 0.18 +0.4 1,825,208 - BBR_RS17995 0.19 -0.8 1,825,257 + BBR_RS17995 0.23 +2.2 1,825,338 - BBR_RS17995 0.29 +0.1 1,825,354 + BBR_RS17995 0.30 -0.4 1,825,354 + BBR_RS17995 0.30 +0.5 1,825,377 + BBR_RS17995 0.32 -0.3 1,825,377 + BBR_RS17995 0.32 +0.2 1,825,377 + BBR_RS17995 0.32 -1.9 1,825,377 + BBR_RS17995 0.32 -0.4 1,825,377 + BBR_RS17995 0.32 +0.2 1,825,377 + BBR_RS17995 0.32 +0.9 1,825,385 - BBR_RS17995 0.32 -0.6 1,825,385 - BBR_RS17995 0.32 +0.0 1,825,385 - BBR_RS17995 0.32 -1.1 1,825,385 - BBR_RS17995 0.32 +0.7 1,825,385 - BBR_RS17995 0.32 +0.6 1,825,400 - BBR_RS17995 0.34 -0.3 1,825,469 + BBR_RS17995 0.39 -0.3 1,825,475 - BBR_RS17995 0.39 -1.1 1,825,477 - BBR_RS17995 0.40 -0.8 1,825,487 - BBR_RS17995 0.40 -0.3 1,825,487 - BBR_RS17995 0.40 -0.6 1,825,510 + BBR_RS17995 0.42 -0.0 1,825,522 + BBR_RS17995 0.43 -0.1 1,825,526 + BBR_RS17995 0.43 -1.1 1,825,534 - BBR_RS17995 0.44 +2.5 1,825,617 + BBR_RS17995 0.50 -0.5 1,825,618 - BBR_RS17995 0.51 +0.1 1,825,656 + BBR_RS17995 0.53 -1.0 1,825,760 + BBR_RS17995 0.61 -0.0 1,825,827 + BBR_RS17995 0.67 -0.7 1,825,835 - BBR_RS17995 0.67 -0.3 1,825,835 - BBR_RS17995 0.67 -2.1 1,825,836 - BBR_RS17995 0.67 -0.9 1,825,859 + BBR_RS17995 0.69 -0.4 1,825,867 - BBR_RS17995 0.70 -1.0 1,825,879 - BBR_RS17995 0.71 -2.4 1,825,932 + BBR_RS17995 0.75 +0.0 1,825,979 - BBR_RS17995 0.78 +0.2 1,826,108 + BBR_RS17995 0.88 +0.7 1,826,113 + BBR_RS17995 0.89 -1.0 1,826,162 - -1.9 1,826,165 - -2.9 1,826,181 - -0.1 1,826,272 - -0.2 1,826,272 - +1.8 1,826,281 - -0.2 1,826,326 + -0.6 1,826,332 - -0.9 1,826,462 + +0.1 1,826,482 - -0.3
Or see this region's nucleotide sequence