Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17205 and BBR_RS17210 are separated by 37 nucleotides BBR_RS17210 and BBR_RS17215 are separated by 126 nucleotides BBR_RS17215 and BBR_RS17220 are separated by 63 nucleotides
BBR_RS17205: BBR_RS17205 - phosphomethylpyrimidine synthase ThiC, at 1,636,689 to 1,639,442
_RS17205
BBR_RS17210: BBR_RS17210 - 26S protease regulatory subunit, at 1,639,480 to 1,640,685
_RS17210
BBR_RS17215: BBR_RS17215 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,640,812 to 1,641,621
_RS17215
BBR_RS17220: BBR_RS17220 - thiamine-binding protein, at 1,641,685 to 1,642,062
_RS17220
Position (kb)
1639
1640
1641 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1638.526 kb on - strand, within BBR_RS17205 at 1638.684 kb on - strand, within BBR_RS17205 at 1638.704 kb on + strand, within BBR_RS17205 at 1638.704 kb on + strand, within BBR_RS17205 at 1638.704 kb on + strand, within BBR_RS17205 at 1638.720 kb on - strand, within BBR_RS17205 at 1638.749 kb on + strand, within BBR_RS17205 at 1638.749 kb on + strand, within BBR_RS17205 at 1638.749 kb on + strand, within BBR_RS17205 at 1638.757 kb on - strand, within BBR_RS17205 at 1638.757 kb on - strand, within BBR_RS17205 at 1638.779 kb on + strand, within BBR_RS17205 at 1638.794 kb on + strand, within BBR_RS17205 at 1638.794 kb on + strand, within BBR_RS17205 at 1638.805 kb on - strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.806 kb on + strand, within BBR_RS17205 at 1638.814 kb on + strand, within BBR_RS17205 at 1638.814 kb on + strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.814 kb on - strand, within BBR_RS17205 at 1638.822 kb on - strand, within BBR_RS17205 at 1638.832 kb on - strand, within BBR_RS17205 at 1638.832 kb on - strand, within BBR_RS17205 at 1638.861 kb on + strand, within BBR_RS17205 at 1638.893 kb on + strand, within BBR_RS17205 at 1638.901 kb on + strand, within BBR_RS17205 at 1638.906 kb on + strand, within BBR_RS17205 at 1638.909 kb on - strand, within BBR_RS17205 at 1638.909 kb on - strand, within BBR_RS17205 at 1638.909 kb on - strand, within BBR_RS17205 at 1638.909 kb on - strand, within BBR_RS17205 at 1638.909 kb on - strand, within BBR_RS17205 at 1638.911 kb on + strand, within BBR_RS17205 at 1638.911 kb on + strand, within BBR_RS17205 at 1638.911 kb on - strand, within BBR_RS17205 at 1638.914 kb on - strand, within BBR_RS17205 at 1638.934 kb on + strand, within BBR_RS17205 at 1638.965 kb on - strand, within BBR_RS17205 at 1638.966 kb on - strand, within BBR_RS17205 at 1638.968 kb on + strand, within BBR_RS17205 at 1639.029 kb on + strand, within BBR_RS17205 at 1639.087 kb on + strand, within BBR_RS17205 at 1639.100 kb on - strand, within BBR_RS17205 at 1639.103 kb on - strand, within BBR_RS17205 at 1639.124 kb on - strand, within BBR_RS17205 at 1639.138 kb on - strand, within BBR_RS17205 at 1639.162 kb on - strand, within BBR_RS17205 at 1639.162 kb on - strand, within BBR_RS17205 at 1639.283 kb on - strand at 1639.291 kb on - strand at 1639.291 kb on - strand at 1639.301 kb on + strand at 1639.301 kb on + strand at 1639.309 kb on - strand at 1639.309 kb on - strand at 1639.309 kb on - strand at 1639.309 kb on - strand at 1639.309 kb on - strand at 1639.309 kb on - strand at 1639.313 kb on + strand at 1639.321 kb on - strand at 1639.327 kb on + strand at 1639.337 kb on - strand at 1639.380 kb on + strand at 1639.404 kb on + strand at 1639.404 kb on + strand at 1639.412 kb on - strand at 1639.412 kb on - strand at 1639.412 kb on - strand at 1639.421 kb on + strand at 1639.421 kb on + strand at 1639.616 kb on - strand, within BBR_RS17210 at 1639.624 kb on + strand, within BBR_RS17210 at 1639.679 kb on + strand, within BBR_RS17210 at 1639.693 kb on - strand, within BBR_RS17210 at 1639.693 kb on - strand, within BBR_RS17210 at 1639.755 kb on - strand, within BBR_RS17210 at 1639.768 kb on - strand, within BBR_RS17210 at 1639.768 kb on - strand, within BBR_RS17210 at 1639.785 kb on + strand, within BBR_RS17210 at 1639.817 kb on + strand, within BBR_RS17210 at 1639.820 kb on - strand, within BBR_RS17210 at 1639.820 kb on - strand, within BBR_RS17210 at 1639.850 kb on + strand, within BBR_RS17210 at 1639.891 kb on - strand, within BBR_RS17210 at 1640.028 kb on - strand, within BBR_RS17210 at 1640.036 kb on + strand, within BBR_RS17210 at 1640.042 kb on - strand, within BBR_RS17210 at 1640.044 kb on - strand, within BBR_RS17210 at 1640.044 kb on - strand, within BBR_RS17210 at 1640.044 kb on - strand, within BBR_RS17210 at 1640.120 kb on + strand, within BBR_RS17210 at 1640.128 kb on - strand, within BBR_RS17210 at 1640.130 kb on + strand, within BBR_RS17210 at 1640.130 kb on + strand, within BBR_RS17210 at 1640.138 kb on - strand, within BBR_RS17210 at 1640.138 kb on - strand, within BBR_RS17210 at 1640.159 kb on + strand, within BBR_RS17210 at 1640.159 kb on - strand, within BBR_RS17210 at 1640.192 kb on - strand, within BBR_RS17210 at 1640.192 kb on - strand, within BBR_RS17210 at 1640.192 kb on - strand, within BBR_RS17210 at 1640.206 kb on - strand, within BBR_RS17210 at 1640.206 kb on - strand, within BBR_RS17210 at 1640.216 kb on - strand, within BBR_RS17210 at 1640.252 kb on - strand, within BBR_RS17210 at 1640.261 kb on + strand, within BBR_RS17210 at 1640.269 kb on - strand, within BBR_RS17210 at 1640.293 kb on + strand, within BBR_RS17210 at 1640.320 kb on - strand, within BBR_RS17210 at 1640.328 kb on - strand, within BBR_RS17210 at 1640.328 kb on - strand, within BBR_RS17210 at 1640.336 kb on + strand, within BBR_RS17210 at 1640.336 kb on + strand, within BBR_RS17210 at 1640.363 kb on + strand, within BBR_RS17210 at 1640.390 kb on - strand, within BBR_RS17210 at 1640.404 kb on - strand, within BBR_RS17210 at 1640.404 kb on - strand, within BBR_RS17210 at 1640.404 kb on - strand, within BBR_RS17210 at 1640.406 kb on + strand, within BBR_RS17210 at 1640.414 kb on - strand, within BBR_RS17210 at 1640.414 kb on - strand, within BBR_RS17210 at 1640.414 kb on - strand, within BBR_RS17210 at 1640.414 kb on - strand, within BBR_RS17210 at 1640.423 kb on - strand, within BBR_RS17210 at 1640.425 kb on + strand, within BBR_RS17210 at 1640.425 kb on + strand, within BBR_RS17210 at 1640.431 kb on - strand, within BBR_RS17210 at 1640.438 kb on + strand, within BBR_RS17210 at 1640.492 kb on - strand, within BBR_RS17210 at 1640.492 kb on - strand, within BBR_RS17210 at 1640.574 kb on + strand at 1640.585 kb on - strand at 1640.596 kb on - strand at 1640.597 kb on - strand at 1640.625 kb on - strand at 1640.682 kb on - strand at 1640.682 kb on - strand at 1640.689 kb on - strand at 1640.689 kb on - strand at 1640.694 kb on - strand at 1640.694 kb on - strand at 1640.737 kb on - strand at 1640.737 kb on - strand at 1640.739 kb on + strand at 1640.803 kb on - strand at 1640.820 kb on + strand at 1640.836 kb on - strand at 1640.836 kb on - strand at 1640.856 kb on + strand at 1640.856 kb on + strand at 1640.878 kb on - strand at 1640.908 kb on + strand, within BBR_RS17215 at 1640.913 kb on + strand, within BBR_RS17215 at 1640.915 kb on - strand, within BBR_RS17215 at 1640.921 kb on - strand, within BBR_RS17215 at 1640.925 kb on + strand, within BBR_RS17215 at 1640.935 kb on + strand, within BBR_RS17215 at 1640.962 kb on - strand, within BBR_RS17215 at 1640.962 kb on - strand, within BBR_RS17215 at 1641.021 kb on - strand, within BBR_RS17215 at 1641.070 kb on + strand, within BBR_RS17215 at 1641.146 kb on + strand, within BBR_RS17215 at 1641.185 kb on - strand, within BBR_RS17215 at 1641.216 kb on + strand, within BBR_RS17215 at 1641.216 kb on + strand, within BBR_RS17215 at 1641.224 kb on - strand, within BBR_RS17215 at 1641.225 kb on + strand, within BBR_RS17215 at 1641.246 kb on - strand, within BBR_RS17215 at 1641.246 kb on - strand, within BBR_RS17215 at 1641.262 kb on + strand, within BBR_RS17215 at 1641.294 kb on + strand, within BBR_RS17215 at 1641.301 kb on - strand, within BBR_RS17215 at 1641.303 kb on - strand, within BBR_RS17215 at 1641.305 kb on + strand, within BBR_RS17215 at 1641.305 kb on + strand, within BBR_RS17215 at 1641.338 kb on + strand, within BBR_RS17215 at 1641.343 kb on - strand, within BBR_RS17215 at 1641.346 kb on - strand, within BBR_RS17215 at 1641.348 kb on + strand, within BBR_RS17215 at 1641.348 kb on + strand, within BBR_RS17215 at 1641.348 kb on + strand, within BBR_RS17215 at 1641.348 kb on + strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.356 kb on - strand, within BBR_RS17215 at 1641.375 kb on + strand, within BBR_RS17215 at 1641.399 kb on + strand, within BBR_RS17215 at 1641.399 kb on + strand, within BBR_RS17215 at 1641.400 kb on + strand, within BBR_RS17215 at 1641.485 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.513 kb on + strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.521 kb on - strand, within BBR_RS17215 at 1641.553 kb on + strand at 1641.563 kb on - strand at 1641.575 kb on - strand at 1641.635 kb on - strand at 1641.643 kb on + strand at 1641.643 kb on + strand at 1641.658 kb on + strand at 1641.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 1,638,526 - BBR_RS17205 0.67 -0.9 1,638,684 - BBR_RS17205 0.72 +1.0 1,638,704 + BBR_RS17205 0.73 +1.1 1,638,704 + BBR_RS17205 0.73 -1.5 1,638,704 + BBR_RS17205 0.73 +0.7 1,638,720 - BBR_RS17205 0.74 +0.6 1,638,749 + BBR_RS17205 0.75 +1.1 1,638,749 + BBR_RS17205 0.75 -0.9 1,638,749 + BBR_RS17205 0.75 +0.5 1,638,757 - BBR_RS17205 0.75 +1.9 1,638,757 - BBR_RS17205 0.75 +1.3 1,638,779 + BBR_RS17205 0.76 -0.6 1,638,794 + BBR_RS17205 0.76 -0.4 1,638,794 + BBR_RS17205 0.76 -0.8 1,638,805 - BBR_RS17205 0.77 +1.1 1,638,806 + BBR_RS17205 0.77 +1.1 1,638,806 + BBR_RS17205 0.77 -0.2 1,638,806 + BBR_RS17205 0.77 +0.1 1,638,806 + BBR_RS17205 0.77 +0.9 1,638,806 + BBR_RS17205 0.77 +0.3 1,638,806 + BBR_RS17205 0.77 -0.2 1,638,806 + BBR_RS17205 0.77 -0.3 1,638,806 + BBR_RS17205 0.77 -0.9 1,638,814 + BBR_RS17205 0.77 -0.8 1,638,814 + BBR_RS17205 0.77 +0.1 1,638,814 - BBR_RS17205 0.77 -1.0 1,638,814 - BBR_RS17205 0.77 -0.8 1,638,814 - BBR_RS17205 0.77 +0.7 1,638,814 - BBR_RS17205 0.77 +2.2 1,638,814 - BBR_RS17205 0.77 +2.9 1,638,814 - BBR_RS17205 0.77 +0.0 1,638,822 - BBR_RS17205 0.77 +0.9 1,638,832 - BBR_RS17205 0.78 +0.8 1,638,832 - BBR_RS17205 0.78 -0.6 1,638,861 + BBR_RS17205 0.79 -0.0 1,638,893 + BBR_RS17205 0.80 +0.4 1,638,901 + BBR_RS17205 0.80 -0.7 1,638,906 + BBR_RS17205 0.81 -1.6 1,638,909 - BBR_RS17205 0.81 -0.6 1,638,909 - BBR_RS17205 0.81 -0.9 1,638,909 - BBR_RS17205 0.81 +1.6 1,638,909 - BBR_RS17205 0.81 +1.1 1,638,909 - BBR_RS17205 0.81 +1.5 1,638,911 + BBR_RS17205 0.81 -0.2 1,638,911 + BBR_RS17205 0.81 -0.0 1,638,911 - BBR_RS17205 0.81 +2.6 1,638,914 - BBR_RS17205 0.81 -1.9 1,638,934 + BBR_RS17205 0.82 +0.9 1,638,965 - BBR_RS17205 0.83 -0.6 1,638,966 - BBR_RS17205 0.83 -0.1 1,638,968 + BBR_RS17205 0.83 +1.9 1,639,029 + BBR_RS17205 0.85 -0.1 1,639,087 + BBR_RS17205 0.87 -1.5 1,639,100 - BBR_RS17205 0.88 -0.4 1,639,103 - BBR_RS17205 0.88 -1.8 1,639,124 - BBR_RS17205 0.88 +0.7 1,639,138 - BBR_RS17205 0.89 +2.0 1,639,162 - BBR_RS17205 0.90 -1.6 1,639,162 - BBR_RS17205 0.90 -0.6 1,639,283 - +1.2 1,639,291 - +0.7 1,639,291 - -1.3 1,639,301 + +0.8 1,639,301 + -1.1 1,639,309 - +1.0 1,639,309 - -1.0 1,639,309 - +2.1 1,639,309 - -0.0 1,639,309 - -1.9 1,639,309 - -1.9 1,639,313 + -0.1 1,639,321 - -1.1 1,639,327 + +0.5 1,639,337 - -1.7 1,639,380 + +2.9 1,639,404 + +2.0 1,639,404 + -1.1 1,639,412 - -0.6 1,639,412 - +1.7 1,639,412 - +1.7 1,639,421 + +1.7 1,639,421 + -0.3 1,639,616 - BBR_RS17210 0.11 +0.5 1,639,624 + BBR_RS17210 0.12 +2.2 1,639,679 + BBR_RS17210 0.17 +1.4 1,639,693 - BBR_RS17210 0.18 -0.0 1,639,693 - BBR_RS17210 0.18 +0.3 1,639,755 - BBR_RS17210 0.23 +1.7 1,639,768 - BBR_RS17210 0.24 +1.0 1,639,768 - BBR_RS17210 0.24 +1.6 1,639,785 + BBR_RS17210 0.25 -0.3 1,639,817 + BBR_RS17210 0.28 -0.2 1,639,820 - BBR_RS17210 0.28 -0.3 1,639,820 - BBR_RS17210 0.28 -1.6 1,639,850 + BBR_RS17210 0.31 +0.8 1,639,891 - BBR_RS17210 0.34 +1.0 1,640,028 - BBR_RS17210 0.45 +2.2 1,640,036 + BBR_RS17210 0.46 -0.1 1,640,042 - BBR_RS17210 0.47 +1.5 1,640,044 - BBR_RS17210 0.47 +1.5 1,640,044 - BBR_RS17210 0.47 +1.6 1,640,044 - BBR_RS17210 0.47 +1.3 1,640,120 + BBR_RS17210 0.53 -0.1 1,640,128 - BBR_RS17210 0.54 +0.3 1,640,130 + BBR_RS17210 0.54 -1.0 1,640,130 + BBR_RS17210 0.54 +0.5 1,640,138 - BBR_RS17210 0.55 -1.0 1,640,138 - BBR_RS17210 0.55 +1.2 1,640,159 + BBR_RS17210 0.56 +0.4 1,640,159 - BBR_RS17210 0.56 -0.8 1,640,192 - BBR_RS17210 0.59 +1.2 1,640,192 - BBR_RS17210 0.59 +0.9 1,640,192 - BBR_RS17210 0.59 +1.6 1,640,206 - BBR_RS17210 0.60 -0.3 1,640,206 - BBR_RS17210 0.60 +0.8 1,640,216 - BBR_RS17210 0.61 +0.6 1,640,252 - BBR_RS17210 0.64 -0.8 1,640,261 + BBR_RS17210 0.65 +0.4 1,640,269 - BBR_RS17210 0.65 +0.6 1,640,293 + BBR_RS17210 0.67 +0.7 1,640,320 - BBR_RS17210 0.70 +1.1 1,640,328 - BBR_RS17210 0.70 +0.3 1,640,328 - BBR_RS17210 0.70 -0.1 1,640,336 + BBR_RS17210 0.71 -1.2 1,640,336 + BBR_RS17210 0.71 +1.3 1,640,363 + BBR_RS17210 0.73 +0.1 1,640,390 - BBR_RS17210 0.75 -1.1 1,640,404 - BBR_RS17210 0.77 -1.7 1,640,404 - BBR_RS17210 0.77 +2.8 1,640,404 - BBR_RS17210 0.77 +0.4 1,640,406 + BBR_RS17210 0.77 -0.3 1,640,414 - BBR_RS17210 0.77 +3.3 1,640,414 - BBR_RS17210 0.77 +0.3 1,640,414 - BBR_RS17210 0.77 +0.3 1,640,414 - BBR_RS17210 0.77 -1.8 1,640,423 - BBR_RS17210 0.78 -1.8 1,640,425 + BBR_RS17210 0.78 -0.6 1,640,425 + BBR_RS17210 0.78 +1.3 1,640,431 - BBR_RS17210 0.79 -1.9 1,640,438 + BBR_RS17210 0.79 -0.4 1,640,492 - BBR_RS17210 0.84 +1.0 1,640,492 - BBR_RS17210 0.84 +0.9 1,640,574 + +0.4 1,640,585 - +1.7 1,640,596 - -0.4 1,640,597 - -0.2 1,640,625 - +1.0 1,640,682 - +0.5 1,640,682 - +1.4 1,640,689 - +0.7 1,640,689 - -1.7 1,640,694 - -2.4 1,640,694 - +0.2 1,640,737 - +0.0 1,640,737 - +0.4 1,640,739 + -1.1 1,640,803 - -0.2 1,640,820 + +1.0 1,640,836 - -0.1 1,640,836 - +0.7 1,640,856 + +2.1 1,640,856 + +1.1 1,640,878 - -0.6 1,640,908 + BBR_RS17215 0.12 +2.0 1,640,913 + BBR_RS17215 0.12 +0.0 1,640,915 - BBR_RS17215 0.13 -0.3 1,640,921 - BBR_RS17215 0.13 +3.9 1,640,925 + BBR_RS17215 0.14 -0.5 1,640,935 + BBR_RS17215 0.15 +0.0 1,640,962 - BBR_RS17215 0.19 +0.3 1,640,962 - BBR_RS17215 0.19 +0.3 1,641,021 - BBR_RS17215 0.26 +0.9 1,641,070 + BBR_RS17215 0.32 +2.1 1,641,146 + BBR_RS17215 0.41 -0.6 1,641,185 - BBR_RS17215 0.46 -0.8 1,641,216 + BBR_RS17215 0.50 +2.2 1,641,216 + BBR_RS17215 0.50 -1.0 1,641,224 - BBR_RS17215 0.51 +0.1 1,641,225 + BBR_RS17215 0.51 +0.9 1,641,246 - BBR_RS17215 0.54 -0.9 1,641,246 - BBR_RS17215 0.54 -1.1 1,641,262 + BBR_RS17215 0.56 +0.3 1,641,294 + BBR_RS17215 0.60 -0.5 1,641,301 - BBR_RS17215 0.60 +0.2 1,641,303 - BBR_RS17215 0.61 -0.7 1,641,305 + BBR_RS17215 0.61 +2.4 1,641,305 + BBR_RS17215 0.61 +0.7 1,641,338 + BBR_RS17215 0.65 -0.1 1,641,343 - BBR_RS17215 0.66 +0.8 1,641,346 - BBR_RS17215 0.66 +1.9 1,641,348 + BBR_RS17215 0.66 +4.9 1,641,348 + BBR_RS17215 0.66 +0.9 1,641,348 + BBR_RS17215 0.66 +1.0 1,641,348 + BBR_RS17215 0.66 +3.5 1,641,356 - BBR_RS17215 0.67 -0.6 1,641,356 - BBR_RS17215 0.67 +2.4 1,641,356 - BBR_RS17215 0.67 +1.4 1,641,356 - BBR_RS17215 0.67 +1.7 1,641,356 - BBR_RS17215 0.67 +2.5 1,641,356 - BBR_RS17215 0.67 +0.7 1,641,375 + BBR_RS17215 0.70 +1.2 1,641,399 + BBR_RS17215 0.72 -0.6 1,641,399 + BBR_RS17215 0.72 +0.6 1,641,400 + BBR_RS17215 0.73 -0.5 1,641,485 + BBR_RS17215 0.83 +0.9 1,641,513 + BBR_RS17215 0.87 -1.2 1,641,513 + BBR_RS17215 0.87 -1.8 1,641,513 + BBR_RS17215 0.87 +0.6 1,641,521 - BBR_RS17215 0.88 +2.5 1,641,521 - BBR_RS17215 0.88 +1.8 1,641,521 - BBR_RS17215 0.88 +1.0 1,641,553 + -0.6 1,641,563 - -1.5 1,641,575 - +3.8 1,641,635 - +1.5 1,641,643 + -1.1 1,641,643 + -2.3 1,641,658 + +0.3 1,641,666 - -0.1
Or see this region's nucleotide sequence