Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15915 and BBR_RS15920 are separated by 3 nucleotides BBR_RS15920 and BBR_RS20960 are separated by 57 nucleotides BBR_RS20960 and BBR_RS15930 are separated by 338 nucleotides BBR_RS15930 and BBR_RS15935 are separated by 33 nucleotides BBR_RS15935 and BBR_RS15940 are separated by 131 nucleotides
BBR_RS15915: BBR_RS15915 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 1,332,787 to 1,333,056
_RS15915
BBR_RS15920: BBR_RS15920 - type II toxin-antitoxin system YafQ family toxin, at 1,333,060 to 1,333,353
_RS15920
BBR_RS20960: BBR_RS20960 - hypothetical protein, at 1,333,411 to 1,333,683
_RS20960
BBR_RS15930: BBR_RS15930 - RNA polymerase sigma factor, at 1,334,022 to 1,334,573
_RS15930
BBR_RS15935: BBR_RS15935 - hypothetical protein, at 1,334,607 to 1,335,434
_RS15935
BBR_RS15940: BBR_RS15940 - hypothetical protein, at 1,335,566 to 1,336,192
_RS15940
Position (kb)
1334
1335 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1333.071 kb on + strand at 1333.071 kb on + strand at 1333.079 kb on - strand at 1333.134 kb on - strand, within BBR_RS15920 at 1333.134 kb on - strand, within BBR_RS15920 at 1333.170 kb on - strand, within BBR_RS15920 at 1333.187 kb on - strand, within BBR_RS15920 at 1333.187 kb on - strand, within BBR_RS15920 at 1333.219 kb on - strand, within BBR_RS15920 at 1333.246 kb on - strand, within BBR_RS15920 at 1333.368 kb on + strand at 1333.421 kb on - strand at 1333.450 kb on - strand, within BBR_RS20960 at 1333.461 kb on + strand, within BBR_RS20960 at 1333.484 kb on - strand, within BBR_RS20960 at 1333.503 kb on - strand, within BBR_RS20960 at 1333.617 kb on - strand, within BBR_RS20960 at 1333.688 kb on - strand at 1333.704 kb on - strand at 1333.706 kb on + strand at 1333.711 kb on + strand at 1333.771 kb on - strand at 1333.786 kb on - strand at 1333.827 kb on - strand at 1333.883 kb on + strand at 1333.883 kb on + strand at 1333.883 kb on + strand at 1333.891 kb on - strand at 1333.920 kb on + strand at 1333.920 kb on + strand at 1334.015 kb on + strand at 1334.019 kb on - strand at 1334.088 kb on - strand, within BBR_RS15930 at 1334.122 kb on + strand, within BBR_RS15930 at 1334.127 kb on - strand, within BBR_RS15930 at 1334.225 kb on + strand, within BBR_RS15930 at 1334.243 kb on + strand, within BBR_RS15930 at 1334.249 kb on + strand, within BBR_RS15930 at 1334.254 kb on + strand, within BBR_RS15930 at 1334.280 kb on + strand, within BBR_RS15930 at 1334.340 kb on - strand, within BBR_RS15930 at 1334.348 kb on - strand, within BBR_RS15930 at 1334.388 kb on + strand, within BBR_RS15930 at 1334.396 kb on - strand, within BBR_RS15930 at 1334.535 kb on + strand at 1334.608 kb on + strand at 1334.764 kb on - strand, within BBR_RS15935 at 1334.768 kb on - strand, within BBR_RS15935 at 1334.794 kb on + strand, within BBR_RS15935 at 1334.804 kb on + strand, within BBR_RS15935 at 1334.836 kb on - strand, within BBR_RS15935 at 1334.853 kb on + strand, within BBR_RS15935 at 1334.863 kb on + strand, within BBR_RS15935 at 1334.928 kb on + strand, within BBR_RS15935 at 1334.933 kb on + strand, within BBR_RS15935 at 1334.947 kb on + strand, within BBR_RS15935 at 1334.999 kb on + strand, within BBR_RS15935 at 1335.027 kb on + strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.138 kb on - strand, within BBR_RS15935 at 1335.138 kb on - strand, within BBR_RS15935 at 1335.153 kb on - strand, within BBR_RS15935 at 1335.164 kb on + strand, within BBR_RS15935 at 1335.190 kb on - strand, within BBR_RS15935 at 1335.278 kb on + strand, within BBR_RS15935 at 1335.324 kb on - strand, within BBR_RS15935 at 1335.397 kb on - strand at 1335.401 kb on + strand at 1335.445 kb on + strand at 1335.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 1,333,071 + -0.0 1,333,071 + +0.2 1,333,079 - +2.1 1,333,134 - BBR_RS15920 0.25 +0.4 1,333,134 - BBR_RS15920 0.25 +0.0 1,333,170 - BBR_RS15920 0.37 -0.4 1,333,187 - BBR_RS15920 0.43 +0.1 1,333,187 - BBR_RS15920 0.43 -0.7 1,333,219 - BBR_RS15920 0.54 +0.1 1,333,246 - BBR_RS15920 0.63 +0.1 1,333,368 + +0.6 1,333,421 - -0.8 1,333,450 - BBR_RS20960 0.14 +1.2 1,333,461 + BBR_RS20960 0.18 -0.7 1,333,484 - BBR_RS20960 0.27 -1.3 1,333,503 - BBR_RS20960 0.34 -0.3 1,333,617 - BBR_RS20960 0.75 -0.3 1,333,688 - +1.9 1,333,704 - -0.0 1,333,706 + +0.2 1,333,711 + +0.1 1,333,771 - +1.3 1,333,786 - +1.1 1,333,827 - +1.3 1,333,883 + -0.6 1,333,883 + -0.6 1,333,883 + -0.9 1,333,891 - +1.7 1,333,920 + -2.0 1,333,920 + +0.8 1,334,015 + +1.2 1,334,019 - -1.0 1,334,088 - BBR_RS15930 0.12 +0.3 1,334,122 + BBR_RS15930 0.18 +2.3 1,334,127 - BBR_RS15930 0.19 -0.6 1,334,225 + BBR_RS15930 0.37 +0.9 1,334,243 + BBR_RS15930 0.40 +0.1 1,334,249 + BBR_RS15930 0.41 -0.3 1,334,254 + BBR_RS15930 0.42 +0.9 1,334,280 + BBR_RS15930 0.47 +1.2 1,334,340 - BBR_RS15930 0.58 -1.3 1,334,348 - BBR_RS15930 0.59 -0.5 1,334,388 + BBR_RS15930 0.66 -2.7 1,334,396 - BBR_RS15930 0.68 +2.4 1,334,535 + -1.1 1,334,608 + -0.8 1,334,764 - BBR_RS15935 0.19 +1.4 1,334,768 - BBR_RS15935 0.19 +2.2 1,334,794 + BBR_RS15935 0.23 +0.1 1,334,804 + BBR_RS15935 0.24 +0.1 1,334,836 - BBR_RS15935 0.28 +1.2 1,334,853 + BBR_RS15935 0.30 +1.9 1,334,863 + BBR_RS15935 0.31 -0.6 1,334,928 + BBR_RS15935 0.39 +0.3 1,334,933 + BBR_RS15935 0.39 -0.9 1,334,947 + BBR_RS15935 0.41 -0.0 1,334,999 + BBR_RS15935 0.47 +1.3 1,335,027 + BBR_RS15935 0.51 +0.1 1,335,105 - BBR_RS15935 0.60 +3.8 1,335,105 - BBR_RS15935 0.60 +0.9 1,335,105 - BBR_RS15935 0.60 +2.5 1,335,138 - BBR_RS15935 0.64 +0.6 1,335,138 - BBR_RS15935 0.64 +0.2 1,335,153 - BBR_RS15935 0.66 +0.6 1,335,164 + BBR_RS15935 0.67 -0.4 1,335,190 - BBR_RS15935 0.70 +2.2 1,335,278 + BBR_RS15935 0.81 -2.2 1,335,324 - BBR_RS15935 0.87 +0.9 1,335,397 - +0.9 1,335,401 + -0.1 1,335,445 + +1.3 1,335,483 + +0.8
Or see this region's nucleotide sequence