Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15205 and BBR_RS15210 are separated by 223 nucleotides BBR_RS15210 and BBR_RS15215 are separated by 198 nucleotides
BBR_RS15205: BBR_RS15205 - 50S ribosomal protein L25, at 1,180,009 to 1,180,638
_RS15205
BBR_RS15210: BBR_RS15210 - branched-chain amino acid aminotransferase, at 1,180,862 to 1,181,989
_RS15210
BBR_RS15215: BBR_RS15215 - MazG-like protein, at 1,182,188 to 1,183,069
_RS15215
Position (kb)
1180
1181
1182 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1180.242 kb on + strand, within BBR_RS15205 at 1180.334 kb on + strand, within BBR_RS15205 at 1180.580 kb on + strand at 1180.757 kb on + strand at 1180.757 kb on + strand at 1180.757 kb on + strand at 1180.785 kb on - strand at 1180.815 kb on - strand at 1180.920 kb on + strand at 1180.920 kb on + strand at 1180.939 kb on + strand at 1181.074 kb on + strand, within BBR_RS15210 at 1181.104 kb on + strand, within BBR_RS15210 at 1181.129 kb on - strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.131 kb on + strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.139 kb on - strand, within BBR_RS15210 at 1181.173 kb on - strand, within BBR_RS15210 at 1181.238 kb on + strand, within BBR_RS15210 at 1181.272 kb on + strand, within BBR_RS15210 at 1181.272 kb on + strand, within BBR_RS15210 at 1181.318 kb on + strand, within BBR_RS15210 at 1181.346 kb on - strand, within BBR_RS15210 at 1181.381 kb on + strand, within BBR_RS15210 at 1181.411 kb on + strand, within BBR_RS15210 at 1181.523 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.539 kb on + strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.547 kb on - strand, within BBR_RS15210 at 1181.577 kb on - strand, within BBR_RS15210 at 1181.626 kb on + strand, within BBR_RS15210 at 1181.626 kb on + strand, within BBR_RS15210 at 1181.636 kb on + strand, within BBR_RS15210 at 1181.636 kb on + strand, within BBR_RS15210 at 1181.725 kb on + strand, within BBR_RS15210 at 1181.744 kb on + strand, within BBR_RS15210 at 1181.744 kb on + strand, within BBR_RS15210 at 1181.790 kb on - strand, within BBR_RS15210 at 1181.907 kb on + strand at 1181.948 kb on - strand at 1181.948 kb on - strand at 1181.958 kb on - strand at 1182.009 kb on + strand at 1182.019 kb on + strand at 1182.135 kb on + strand at 1182.145 kb on + strand at 1182.148 kb on - strand at 1182.196 kb on + strand at 1182.217 kb on + strand at 1182.239 kb on - strand at 1182.254 kb on - strand at 1182.277 kb on + strand, within BBR_RS15215 at 1182.301 kb on - strand, within BBR_RS15215 at 1182.304 kb on + strand, within BBR_RS15215 at 1182.304 kb on + strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.312 kb on - strand, within BBR_RS15215 at 1182.428 kb on + strand, within BBR_RS15215 at 1182.512 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.528 kb on + strand, within BBR_RS15215 at 1182.536 kb on - strand, within BBR_RS15215 at 1182.728 kb on - strand, within BBR_RS15215 at 1182.744 kb on + strand, within BBR_RS15215 at 1182.750 kb on - strand, within BBR_RS15215 at 1182.794 kb on - strand, within BBR_RS15215 at 1182.830 kb on - strand, within BBR_RS15215 at 1182.858 kb on + strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.866 kb on - strand, within BBR_RS15215 at 1182.927 kb on - strand, within BBR_RS15215 at 1182.956 kb on + strand, within BBR_RS15215
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 1,180,242 + BBR_RS15205 0.37 +2.8 1,180,334 + BBR_RS15205 0.52 +2.2 1,180,580 + +1.5 1,180,757 + -1.3 1,180,757 + +1.0 1,180,757 + -1.4 1,180,785 - -2.6 1,180,815 - -0.5 1,180,920 + +1.1 1,180,920 + -1.6 1,180,939 + +1.1 1,181,074 + BBR_RS15210 0.19 -0.9 1,181,104 + BBR_RS15210 0.21 -0.4 1,181,129 - BBR_RS15210 0.24 +2.0 1,181,131 + BBR_RS15210 0.24 +1.2 1,181,131 + BBR_RS15210 0.24 -1.2 1,181,131 + BBR_RS15210 0.24 +2.2 1,181,131 + BBR_RS15210 0.24 +1.0 1,181,139 - BBR_RS15210 0.25 +0.9 1,181,139 - BBR_RS15210 0.25 -0.1 1,181,139 - BBR_RS15210 0.25 -0.8 1,181,173 - BBR_RS15210 0.28 +2.2 1,181,238 + BBR_RS15210 0.33 -1.4 1,181,272 + BBR_RS15210 0.36 -1.1 1,181,272 + BBR_RS15210 0.36 -1.7 1,181,318 + BBR_RS15210 0.40 -1.4 1,181,346 - BBR_RS15210 0.43 +2.0 1,181,381 + BBR_RS15210 0.46 +1.0 1,181,411 + BBR_RS15210 0.49 +0.3 1,181,523 + BBR_RS15210 0.59 -1.6 1,181,539 + BBR_RS15210 0.60 -0.6 1,181,539 + BBR_RS15210 0.60 -0.5 1,181,539 + BBR_RS15210 0.60 +0.5 1,181,547 - BBR_RS15210 0.61 -0.0 1,181,547 - BBR_RS15210 0.61 -0.4 1,181,547 - BBR_RS15210 0.61 +0.3 1,181,547 - BBR_RS15210 0.61 +0.5 1,181,577 - BBR_RS15210 0.63 +1.6 1,181,626 + BBR_RS15210 0.68 -1.0 1,181,626 + BBR_RS15210 0.68 -0.9 1,181,636 + BBR_RS15210 0.69 +0.3 1,181,636 + BBR_RS15210 0.69 -2.2 1,181,725 + BBR_RS15210 0.77 -1.2 1,181,744 + BBR_RS15210 0.78 -0.3 1,181,744 + BBR_RS15210 0.78 -0.8 1,181,790 - BBR_RS15210 0.82 +1.6 1,181,907 + +0.3 1,181,948 - -0.7 1,181,948 - +0.1 1,181,958 - +2.5 1,182,009 + -0.5 1,182,019 + +0.2 1,182,135 + +0.9 1,182,145 + +0.0 1,182,148 - -1.5 1,182,196 + -1.2 1,182,217 + +2.5 1,182,239 - +0.9 1,182,254 - -1.7 1,182,277 + BBR_RS15215 0.10 +2.6 1,182,301 - BBR_RS15215 0.13 -1.2 1,182,304 + BBR_RS15215 0.13 +0.3 1,182,304 + BBR_RS15215 0.13 -1.0 1,182,312 - BBR_RS15215 0.14 +0.5 1,182,312 - BBR_RS15215 0.14 +0.6 1,182,312 - BBR_RS15215 0.14 +1.5 1,182,428 + BBR_RS15215 0.27 -1.7 1,182,512 + BBR_RS15215 0.37 +0.8 1,182,528 + BBR_RS15215 0.39 -0.6 1,182,528 + BBR_RS15215 0.39 +0.7 1,182,536 - BBR_RS15215 0.39 +0.4 1,182,728 - BBR_RS15215 0.61 -0.2 1,182,744 + BBR_RS15215 0.63 +0.4 1,182,750 - BBR_RS15215 0.64 -0.3 1,182,794 - BBR_RS15215 0.69 +0.1 1,182,830 - BBR_RS15215 0.73 -0.7 1,182,858 + BBR_RS15215 0.76 +0.1 1,182,866 - BBR_RS15215 0.77 +2.1 1,182,866 - BBR_RS15215 0.77 +0.5 1,182,927 - BBR_RS15215 0.84 +0.6 1,182,956 + BBR_RS15215 0.87 -0.1
Or see this region's nucleotide sequence