Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14930 and BBR_RS20875 are separated by 175 nucleotides BBR_RS20875 and BBR_RS14940 are separated by 34 nucleotides BBR_RS14940 and BBR_RS14945 are separated by 193 nucleotides BBR_RS14945 and BBR_RS14950 are separated by 91 nucleotides
BBR_RS14930: BBR_RS14930 - DNA-binding protein, also contains divergent AAA domain for ATP binding (DNA helicase), at 1,123,984 to 1,125,462
_RS14930
BBR_RS20875: BBR_RS20875 - hypothetical protein, at 1,125,638 to 1,126,243
_RS20875
BBR_RS14940: BBR_RS14940 - hypothetical protein, at 1,126,278 to 1,127,498
_RS14940
BBR_RS14945: BBR_RS14945 - peptidase E, at 1,127,692 to 1,128,315
_RS14945
BBR_RS14950: BBR_RS14950 - Pyrazinamidase/Nicotinamidase, at 1,128,407 to 1,129,021
_RS14950
Position (kb)
1126
1127
1128 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1125.300 kb on - strand, within BBR_RS14930 at 1125.301 kb on + strand, within BBR_RS14930 at 1125.336 kb on + strand at 1125.344 kb on - strand at 1125.344 kb on - strand at 1125.350 kb on + strand at 1125.380 kb on + strand at 1125.427 kb on - strand at 1125.429 kb on + strand at 1125.561 kb on - strand at 1125.612 kb on + strand at 1125.638 kb on + strand at 1125.642 kb on - strand at 1125.696 kb on + strand at 1125.745 kb on + strand, within BBR_RS20875 at 1125.871 kb on + strand, within BBR_RS20875 at 1125.881 kb on + strand, within BBR_RS20875 at 1125.884 kb on - strand, within BBR_RS20875 at 1125.927 kb on + strand, within BBR_RS20875 at 1125.929 kb on - strand, within BBR_RS20875 at 1125.954 kb on + strand, within BBR_RS20875 at 1125.998 kb on - strand, within BBR_RS20875 at 1126.002 kb on + strand, within BBR_RS20875 at 1126.002 kb on + strand, within BBR_RS20875 at 1126.009 kb on + strand, within BBR_RS20875 at 1126.010 kb on - strand, within BBR_RS20875 at 1126.037 kb on - strand, within BBR_RS20875 at 1126.083 kb on - strand, within BBR_RS20875 at 1126.149 kb on + strand, within BBR_RS20875 at 1126.149 kb on + strand, within BBR_RS20875 at 1126.157 kb on + strand, within BBR_RS20875 at 1126.204 kb on - strand at 1126.246 kb on - strand at 1126.272 kb on - strand at 1126.293 kb on + strand at 1126.299 kb on + strand at 1126.299 kb on + strand at 1126.354 kb on - strand at 1126.408 kb on - strand, within BBR_RS14940 at 1126.438 kb on - strand, within BBR_RS14940 at 1126.446 kb on + strand, within BBR_RS14940 at 1126.484 kb on - strand, within BBR_RS14940 at 1126.484 kb on - strand, within BBR_RS14940 at 1126.492 kb on - strand, within BBR_RS14940 at 1126.527 kb on + strand, within BBR_RS14940 at 1126.535 kb on + strand, within BBR_RS14940 at 1126.610 kb on + strand, within BBR_RS14940 at 1126.799 kb on - strand, within BBR_RS14940 at 1126.811 kb on + strand, within BBR_RS14940 at 1126.895 kb on + strand, within BBR_RS14940 at 1126.957 kb on - strand, within BBR_RS14940 at 1127.040 kb on - strand, within BBR_RS14940 at 1127.040 kb on - strand, within BBR_RS14940 at 1127.128 kb on + strand, within BBR_RS14940 at 1127.263 kb on + strand, within BBR_RS14940 at 1127.271 kb on - strand, within BBR_RS14940 at 1127.271 kb on - strand, within BBR_RS14940 at 1127.276 kb on + strand, within BBR_RS14940 at 1127.325 kb on - strand, within BBR_RS14940 at 1127.325 kb on - strand, within BBR_RS14940 at 1127.343 kb on + strand, within BBR_RS14940 at 1127.348 kb on - strand, within BBR_RS14940 at 1127.403 kb on - strand at 1127.426 kb on - strand at 1127.440 kb on - strand at 1127.442 kb on - strand at 1127.442 kb on - strand at 1127.465 kb on + strand at 1127.465 kb on + strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.476 kb on + strand at 1127.487 kb on + strand at 1127.497 kb on + strand at 1127.500 kb on - strand at 1127.558 kb on - strand at 1127.563 kb on + strand at 1127.566 kb on + strand at 1127.566 kb on - strand at 1127.573 kb on + strand at 1127.576 kb on - strand at 1127.592 kb on + strand at 1127.595 kb on - strand at 1127.632 kb on + strand at 1127.650 kb on + strand at 1127.658 kb on - strand at 1127.693 kb on - strand at 1127.771 kb on - strand, within BBR_RS14945 at 1127.776 kb on - strand, within BBR_RS14945 at 1127.801 kb on - strand, within BBR_RS14945 at 1127.803 kb on + strand, within BBR_RS14945 at 1127.805 kb on - strand, within BBR_RS14945 at 1127.811 kb on - strand, within BBR_RS14945 at 1127.851 kb on + strand, within BBR_RS14945 at 1127.859 kb on - strand, within BBR_RS14945 at 1127.859 kb on - strand, within BBR_RS14945 at 1127.874 kb on + strand, within BBR_RS14945 at 1127.881 kb on + strand, within BBR_RS14945 at 1127.904 kb on + strand, within BBR_RS14945 at 1127.920 kb on + strand, within BBR_RS14945 at 1127.954 kb on + strand, within BBR_RS14945 at 1127.956 kb on + strand, within BBR_RS14945 at 1128.006 kb on - strand, within BBR_RS14945 at 1128.072 kb on + strand, within BBR_RS14945 at 1128.151 kb on - strand, within BBR_RS14945 at 1128.164 kb on + strand, within BBR_RS14945 at 1128.206 kb on - strand, within BBR_RS14945 at 1128.214 kb on - strand, within BBR_RS14945 at 1128.286 kb on - strand at 1128.288 kb on + strand at 1128.320 kb on - strand at 1128.324 kb on + strand at 1128.348 kb on + strand at 1128.362 kb on - strand at 1128.390 kb on + strand at 1128.390 kb on + strand at 1128.422 kb on - strand at 1128.432 kb on - strand at 1128.472 kb on - strand, within BBR_RS14950
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 1,125,300 - BBR_RS14930 0.89 +0.2 1,125,301 + BBR_RS14930 0.89 +1.8 1,125,336 + -0.3 1,125,344 - -0.0 1,125,344 - -0.2 1,125,350 + +0.5 1,125,380 + -0.0 1,125,427 - +1.3 1,125,429 + +0.7 1,125,561 - -1.2 1,125,612 + -0.4 1,125,638 + +1.0 1,125,642 - -0.1 1,125,696 + -0.9 1,125,745 + BBR_RS20875 0.18 +1.7 1,125,871 + BBR_RS20875 0.38 -0.1 1,125,881 + BBR_RS20875 0.40 -0.3 1,125,884 - BBR_RS20875 0.41 +1.9 1,125,927 + BBR_RS20875 0.48 +2.1 1,125,929 - BBR_RS20875 0.48 -0.3 1,125,954 + BBR_RS20875 0.52 -1.3 1,125,998 - BBR_RS20875 0.59 -0.1 1,126,002 + BBR_RS20875 0.60 -0.0 1,126,002 + BBR_RS20875 0.60 +1.2 1,126,009 + BBR_RS20875 0.61 -0.7 1,126,010 - BBR_RS20875 0.61 +1.2 1,126,037 - BBR_RS20875 0.66 +0.4 1,126,083 - BBR_RS20875 0.73 -1.2 1,126,149 + BBR_RS20875 0.84 -1.3 1,126,149 + BBR_RS20875 0.84 -1.4 1,126,157 + BBR_RS20875 0.86 +0.3 1,126,204 - +0.8 1,126,246 - +3.1 1,126,272 - -0.1 1,126,293 + -0.7 1,126,299 + -1.6 1,126,299 + +1.7 1,126,354 - -0.4 1,126,408 - BBR_RS14940 0.11 +1.3 1,126,438 - BBR_RS14940 0.13 +0.4 1,126,446 + BBR_RS14940 0.14 -1.4 1,126,484 - BBR_RS14940 0.17 +0.9 1,126,484 - BBR_RS14940 0.17 -0.5 1,126,492 - BBR_RS14940 0.18 -0.4 1,126,527 + BBR_RS14940 0.20 +0.2 1,126,535 + BBR_RS14940 0.21 -1.6 1,126,610 + BBR_RS14940 0.27 +0.8 1,126,799 - BBR_RS14940 0.43 +0.9 1,126,811 + BBR_RS14940 0.44 -1.0 1,126,895 + BBR_RS14940 0.51 +0.2 1,126,957 - BBR_RS14940 0.56 -0.9 1,127,040 - BBR_RS14940 0.62 -0.2 1,127,040 - BBR_RS14940 0.62 -0.8 1,127,128 + BBR_RS14940 0.70 +1.9 1,127,263 + BBR_RS14940 0.81 -0.8 1,127,271 - BBR_RS14940 0.81 -0.1 1,127,271 - BBR_RS14940 0.81 +1.1 1,127,276 + BBR_RS14940 0.82 +0.6 1,127,325 - BBR_RS14940 0.86 -1.1 1,127,325 - BBR_RS14940 0.86 +4.8 1,127,343 + BBR_RS14940 0.87 -2.2 1,127,348 - BBR_RS14940 0.88 +1.2 1,127,403 - -0.4 1,127,426 - -0.6 1,127,440 - +0.3 1,127,442 - +3.1 1,127,442 - +2.1 1,127,465 + -0.1 1,127,465 + +1.2 1,127,473 - -0.1 1,127,473 - +0.3 1,127,473 - -1.3 1,127,473 - +0.7 1,127,473 - +0.0 1,127,473 - +0.3 1,127,476 + +0.2 1,127,487 + -0.4 1,127,497 + -1.0 1,127,500 - -1.1 1,127,558 - +1.5 1,127,563 + +2.0 1,127,566 + -0.3 1,127,566 - +2.4 1,127,573 + -1.4 1,127,576 - -1.5 1,127,592 + +0.2 1,127,595 - -0.4 1,127,632 + -1.8 1,127,650 + +0.4 1,127,658 - +1.8 1,127,693 - +0.9 1,127,771 - BBR_RS14945 0.13 +1.3 1,127,776 - BBR_RS14945 0.13 -1.0 1,127,801 - BBR_RS14945 0.17 -0.3 1,127,803 + BBR_RS14945 0.18 +3.9 1,127,805 - BBR_RS14945 0.18 -0.0 1,127,811 - BBR_RS14945 0.19 -0.1 1,127,851 + BBR_RS14945 0.25 +1.0 1,127,859 - BBR_RS14945 0.27 +1.0 1,127,859 - BBR_RS14945 0.27 +2.1 1,127,874 + BBR_RS14945 0.29 +0.5 1,127,881 + BBR_RS14945 0.30 -0.6 1,127,904 + BBR_RS14945 0.34 +1.1 1,127,920 + BBR_RS14945 0.37 +1.0 1,127,954 + BBR_RS14945 0.42 +1.1 1,127,956 + BBR_RS14945 0.42 -1.5 1,128,006 - BBR_RS14945 0.50 +2.5 1,128,072 + BBR_RS14945 0.61 +0.5 1,128,151 - BBR_RS14945 0.74 -0.5 1,128,164 + BBR_RS14945 0.76 -0.8 1,128,206 - BBR_RS14945 0.82 +0.6 1,128,214 - BBR_RS14945 0.84 -0.5 1,128,286 - +0.7 1,128,288 + +0.2 1,128,320 - +2.4 1,128,324 + +4.8 1,128,348 + +0.4 1,128,362 - +0.3 1,128,390 + -1.3 1,128,390 + +1.1 1,128,422 - -0.1 1,128,432 - -1.5 1,128,472 - BBR_RS14950 0.11 +2.0
Or see this region's nucleotide sequence