Experiment: Recovery broth with Nitrofurantoin 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13790 and BBR_RS13795 are separated by 8 nucleotides BBR_RS13795 and BBR_RS13800 are separated by 82 nucleotides BBR_RS13800 and BBR_RS13805 are separated by 132 nucleotides
BBR_RS13790: BBR_RS13790 - pyridoxal phosphate-dependent aminotransferase, at 866,853 to 868,064
_RS13790
BBR_RS13795: BBR_RS13795 - ABC transporter ATP-binding protein, at 868,073 to 868,858
_RS13795
BBR_RS13800: BBR_RS13800 - hypothetical protein, at 868,941 to 869,120
_RS13800
BBR_RS13805: BBR_RS13805 - hypothetical protein, at 869,253 to 871,502
_RS13805
Position (kb)
868
869 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 867.078 kb on - strand, within BBR_RS13790 at 867.111 kb on + strand, within BBR_RS13790 at 867.122 kb on - strand, within BBR_RS13790 at 867.143 kb on - strand, within BBR_RS13790 at 867.155 kb on - strand, within BBR_RS13790 at 867.180 kb on - strand, within BBR_RS13790 at 867.191 kb on + strand, within BBR_RS13790 at 867.197 kb on - strand, within BBR_RS13790 at 867.221 kb on - strand, within BBR_RS13790 at 867.244 kb on - strand, within BBR_RS13790 at 867.260 kb on + strand, within BBR_RS13790 at 867.277 kb on + strand, within BBR_RS13790 at 867.304 kb on + strand, within BBR_RS13790 at 867.369 kb on + strand, within BBR_RS13790 at 867.471 kb on - strand, within BBR_RS13790 at 867.613 kb on + strand, within BBR_RS13790 at 867.709 kb on - strand, within BBR_RS13790 at 867.726 kb on + strand, within BBR_RS13790 at 867.726 kb on + strand, within BBR_RS13790 at 867.734 kb on + strand, within BBR_RS13790 at 867.736 kb on - strand, within BBR_RS13790 at 867.736 kb on - strand, within BBR_RS13790 at 867.744 kb on + strand, within BBR_RS13790 at 867.811 kb on - strand, within BBR_RS13790 at 867.836 kb on + strand, within BBR_RS13790 at 867.886 kb on - strand, within BBR_RS13790 at 867.906 kb on - strand, within BBR_RS13790 at 867.931 kb on + strand, within BBR_RS13790 at 867.939 kb on - strand, within BBR_RS13790 at 867.939 kb on - strand, within BBR_RS13790 at 867.949 kb on - strand at 868.022 kb on + strand at 868.032 kb on + strand at 868.040 kb on - strand at 868.042 kb on + strand at 868.066 kb on - strand at 868.113 kb on - strand at 868.211 kb on + strand, within BBR_RS13795 at 868.211 kb on + strand, within BBR_RS13795 at 868.271 kb on + strand, within BBR_RS13795 at 868.292 kb on + strand, within BBR_RS13795 at 868.316 kb on + strand, within BBR_RS13795 at 868.324 kb on - strand, within BBR_RS13795 at 868.326 kb on - strand, within BBR_RS13795 at 868.398 kb on + strand, within BBR_RS13795 at 868.457 kb on + strand, within BBR_RS13795 at 868.495 kb on - strand, within BBR_RS13795 at 868.512 kb on - strand, within BBR_RS13795 at 868.573 kb on - strand, within BBR_RS13795 at 868.590 kb on + strand, within BBR_RS13795 at 868.624 kb on + strand, within BBR_RS13795 at 868.630 kb on + strand, within BBR_RS13795 at 868.630 kb on + strand, within BBR_RS13795 at 868.632 kb on - strand, within BBR_RS13795 at 868.642 kb on + strand, within BBR_RS13795 at 868.642 kb on + strand, within BBR_RS13795 at 868.675 kb on - strand, within BBR_RS13795 at 868.745 kb on - strand, within BBR_RS13795 at 868.795 kb on + strand at 868.803 kb on - strand at 868.836 kb on + strand at 868.852 kb on - strand at 868.863 kb on - strand at 868.867 kb on + strand at 868.890 kb on + strand at 868.891 kb on + strand at 868.904 kb on + strand at 868.912 kb on - strand at 868.912 kb on - strand at 868.912 kb on - strand at 868.924 kb on + strand at 868.930 kb on + strand at 868.948 kb on + strand at 868.948 kb on + strand at 869.011 kb on - strand, within BBR_RS13800 at 869.050 kb on + strand, within BBR_RS13800 at 869.054 kb on + strand, within BBR_RS13800 at 869.063 kb on - strand, within BBR_RS13800 at 869.067 kb on + strand, within BBR_RS13800 at 869.075 kb on - strand, within BBR_RS13800 at 869.093 kb on + strand, within BBR_RS13800 at 869.118 kb on - strand at 869.126 kb on + strand at 869.128 kb on + strand at 869.138 kb on - strand at 869.138 kb on - strand at 869.175 kb on - strand at 869.251 kb on + strand at 869.251 kb on + strand at 869.251 kb on + strand at 869.251 kb on + strand at 869.251 kb on + strand at 869.259 kb on - strand at 869.259 kb on - strand at 869.259 kb on - strand at 869.268 kb on - strand at 869.269 kb on - strand at 869.269 kb on - strand at 869.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.05 mM remove 867,078 - BBR_RS13790 0.19 -0.9 867,111 + BBR_RS13790 0.21 +0.7 867,122 - BBR_RS13790 0.22 +0.5 867,143 - BBR_RS13790 0.24 +0.1 867,155 - BBR_RS13790 0.25 -0.4 867,180 - BBR_RS13790 0.27 -0.8 867,191 + BBR_RS13790 0.28 +1.8 867,197 - BBR_RS13790 0.28 +0.3 867,221 - BBR_RS13790 0.30 +0.3 867,244 - BBR_RS13790 0.32 -0.2 867,260 + BBR_RS13790 0.34 -1.0 867,277 + BBR_RS13790 0.35 +1.3 867,304 + BBR_RS13790 0.37 +0.0 867,369 + BBR_RS13790 0.43 +0.4 867,471 - BBR_RS13790 0.51 +0.1 867,613 + BBR_RS13790 0.63 -0.1 867,709 - BBR_RS13790 0.71 +1.5 867,726 + BBR_RS13790 0.72 +0.5 867,726 + BBR_RS13790 0.72 +2.6 867,734 + BBR_RS13790 0.73 +1.5 867,736 - BBR_RS13790 0.73 +0.7 867,736 - BBR_RS13790 0.73 +0.1 867,744 + BBR_RS13790 0.74 -0.1 867,811 - BBR_RS13790 0.79 +0.4 867,836 + BBR_RS13790 0.81 +0.8 867,886 - BBR_RS13790 0.85 +1.5 867,906 - BBR_RS13790 0.87 -1.0 867,931 + BBR_RS13790 0.89 -0.3 867,939 - BBR_RS13790 0.90 +0.9 867,939 - BBR_RS13790 0.90 +0.2 867,949 - +2.1 868,022 + -0.0 868,032 + +0.9 868,040 - +2.1 868,042 + -1.2 868,066 - -0.9 868,113 - +1.0 868,211 + BBR_RS13795 0.18 -1.5 868,211 + BBR_RS13795 0.18 -1.3 868,271 + BBR_RS13795 0.25 -0.3 868,292 + BBR_RS13795 0.28 -0.8 868,316 + BBR_RS13795 0.31 +0.0 868,324 - BBR_RS13795 0.32 -0.1 868,326 - BBR_RS13795 0.32 +1.8 868,398 + BBR_RS13795 0.41 -1.2 868,457 + BBR_RS13795 0.49 +3.0 868,495 - BBR_RS13795 0.54 +2.1 868,512 - BBR_RS13795 0.56 +0.4 868,573 - BBR_RS13795 0.64 +1.0 868,590 + BBR_RS13795 0.66 +2.9 868,624 + BBR_RS13795 0.70 -0.4 868,630 + BBR_RS13795 0.71 +1.3 868,630 + BBR_RS13795 0.71 -0.6 868,632 - BBR_RS13795 0.71 +0.6 868,642 + BBR_RS13795 0.72 +3.1 868,642 + BBR_RS13795 0.72 +0.5 868,675 - BBR_RS13795 0.77 +2.6 868,745 - BBR_RS13795 0.85 +0.4 868,795 + -1.0 868,803 - +0.7 868,836 + -0.0 868,852 - -0.8 868,863 - -0.5 868,867 + +0.2 868,890 + +0.5 868,891 + +2.1 868,904 + +3.9 868,912 - -1.9 868,912 - -0.3 868,912 - -1.2 868,924 + +0.4 868,930 + +1.0 868,948 + -1.0 868,948 + -1.6 869,011 - BBR_RS13800 0.39 +1.0 869,050 + BBR_RS13800 0.61 +1.2 869,054 + BBR_RS13800 0.63 +0.7 869,063 - BBR_RS13800 0.68 -2.0 869,067 + BBR_RS13800 0.70 +0.5 869,075 - BBR_RS13800 0.74 +2.5 869,093 + BBR_RS13800 0.84 +1.7 869,118 - +0.0 869,126 + +1.5 869,128 + +1.9 869,138 - -1.0 869,138 - -0.7 869,175 - +0.4 869,251 + -0.6 869,251 + -0.0 869,251 + +1.1 869,251 + +0.7 869,251 + +0.7 869,259 - -0.6 869,259 - +0.7 869,259 - -1.6 869,268 - -0.3 869,269 - -1.2 869,269 - +1.0 869,269 - +2.2
Or see this region's nucleotide sequence