Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_833 and Ac3H11_834 are separated by 54 nucleotides Ac3H11_834 and Ac3H11_835 are separated by 159 nucleotides Ac3H11_835 and Ac3H11_836 are separated by 44 nucleotides
Ac3H11_833 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 116,717 to 117,511
_833
Ac3H11_834 - putative signal peptide protein, at 117,566 to 118,021
_834
Ac3H11_835 - hypothetical protein, at 118,181 to 118,561
_835
Ac3H11_836 - Ubiquinone biosynthesis monooxygenase UbiB, at 118,606 to 119,946
_836
Position (kb)
118
119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 117.730 kb on - strand, within Ac3H11_834 at 117.735 kb on + strand, within Ac3H11_834 at 117.736 kb on - strand, within Ac3H11_834 at 117.873 kb on + strand, within Ac3H11_834 at 117.873 kb on + strand, within Ac3H11_834 at 117.874 kb on - strand, within Ac3H11_834 at 117.874 kb on - strand, within Ac3H11_834 at 117.874 kb on - strand, within Ac3H11_834 at 117.874 kb on - strand, within Ac3H11_834 at 117.874 kb on - strand, within Ac3H11_834 at 117.995 kb on + strand at 117.996 kb on - strand at 117.996 kb on - strand at 118.092 kb on + strand at 118.092 kb on + strand at 118.092 kb on + strand at 118.092 kb on + strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.093 kb on - strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.555 kb on + strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.556 kb on - strand at 118.880 kb on + strand, within Ac3H11_836 at 118.880 kb on + strand, within Ac3H11_836 at 118.880 kb on + strand, within Ac3H11_836 at 118.880 kb on + strand, within Ac3H11_836 at 118.881 kb on - strand, within Ac3H11_836 at 118.881 kb on - strand, within Ac3H11_836 at 118.881 kb on - strand, within Ac3H11_836 at 118.881 kb on - strand, within Ac3H11_836 at 118.925 kb on + strand at 118.926 kb on - strand, within Ac3H11_836 at 119.339 kb on + strand, within Ac3H11_836 at 119.339 kb on + strand, within Ac3H11_836 at 119.340 kb on - strand, within Ac3H11_836 at 119.340 kb on - strand, within Ac3H11_836 at 119.340 kb on - strand, within Ac3H11_836 at 119.492 kb on + strand, within Ac3H11_836 at 119.492 kb on + strand, within Ac3H11_836 at 119.492 kb on + strand, within Ac3H11_836 at 119.492 kb on + strand, within Ac3H11_836 at 119.493 kb on - strand, within Ac3H11_836
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 117,730 - Ac3H11_834 0.36 +1.7 117,735 + Ac3H11_834 0.37 -0.7 117,736 - Ac3H11_834 0.37 -1.0 117,873 + Ac3H11_834 0.67 +1.7 117,873 + Ac3H11_834 0.67 +0.5 117,874 - Ac3H11_834 0.68 +0.7 117,874 - Ac3H11_834 0.68 +2.1 117,874 - Ac3H11_834 0.68 -1.8 117,874 - Ac3H11_834 0.68 -0.3 117,874 - Ac3H11_834 0.68 +0.4 117,995 + -0.5 117,996 - -3.2 117,996 - -1.1 118,092 + -0.4 118,092 + -0.1 118,092 + -0.2 118,092 + -3.7 118,093 - +0.7 118,093 - -0.7 118,093 - -2.2 118,093 - +0.6 118,093 - -1.2 118,093 - +0.8 118,093 - -0.4 118,555 + +0.1 118,555 + +1.0 118,555 + +1.0 118,555 + +1.6 118,555 + -0.3 118,555 + +0.9 118,555 + +0.6 118,555 + -0.1 118,555 + +0.3 118,555 + -0.0 118,555 + +0.7 118,555 + -0.0 118,555 + -1.4 118,555 + +0.3 118,555 + +1.3 118,555 + +0.5 118,556 - +4.1 118,556 - -1.2 118,556 - +1.7 118,556 - -1.4 118,556 - -0.0 118,556 - +0.1 118,556 - -0.2 118,556 - -0.2 118,556 - +0.1 118,556 - +0.1 118,556 - +0.1 118,556 - +0.5 118,556 - -1.5 118,556 - +0.8 118,556 - +1.6 118,880 + Ac3H11_836 0.20 -0.7 118,880 + Ac3H11_836 0.20 -0.1 118,880 + Ac3H11_836 0.20 -0.4 118,880 + Ac3H11_836 0.20 +1.9 118,881 - Ac3H11_836 0.21 +4.2 118,881 - Ac3H11_836 0.21 +2.9 118,881 - Ac3H11_836 0.21 +1.4 118,881 - Ac3H11_836 0.21 +0.2 118,925 + +1.6 118,926 - Ac3H11_836 0.24 +3.0 119,339 + Ac3H11_836 0.55 +2.2 119,339 + Ac3H11_836 0.55 +0.3 119,340 - Ac3H11_836 0.55 +1.4 119,340 - Ac3H11_836 0.55 +0.4 119,340 - Ac3H11_836 0.55 +0.2 119,492 + Ac3H11_836 0.66 -0.1 119,492 + Ac3H11_836 0.66 -0.9 119,492 + Ac3H11_836 0.66 +0.8 119,492 + Ac3H11_836 0.66 +0.4 119,493 - Ac3H11_836 0.66 -1.1
Or see this region's nucleotide sequence