Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_384 and Ac3H11_385 are separated by 76 nucleotides Ac3H11_385 and Ac3H11_386 are separated by 100 nucleotides Ac3H11_386 and Ac3H11_387 are separated by 250 nucleotides
Ac3H11_384 - possible endonuclease/exonuclease/phosphatase family protein, at 63,343 to 64,386
_384
Ac3H11_385 - Nucleoside-diphosphate-sugar epimerases, at 64,463 to 65,023
_385
Ac3H11_386 - ATP-dependent RNA helicase RhlE, at 65,124 to 66,935
_386
Ac3H11_387 - ABC transporter ATP-binding protein, at 67,186 to 68,847
_387
Position (kb) 65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 64.140 kb on - strand, within Ac3H11_384 at 64.140 kb on - strand, within Ac3H11_384 at 64.277 kb on + strand, within Ac3H11_384 at 64.277 kb on + strand, within Ac3H11_384 at 64.277 kb on + strand, within Ac3H11_384 at 64.277 kb on + strand, within Ac3H11_384 at 64.278 kb on - strand, within Ac3H11_384 at 64.278 kb on - strand, within Ac3H11_384 at 64.278 kb on - strand, within Ac3H11_384 at 64.295 kb on + strand at 64.295 kb on + strand at 64.296 kb on - strand at 64.296 kb on - strand at 64.296 kb on - strand at 64.412 kb on - strand at 64.520 kb on + strand, within Ac3H11_385 at 64.520 kb on + strand, within Ac3H11_385 at 64.520 kb on + strand, within Ac3H11_385 at 64.521 kb on - strand, within Ac3H11_385 at 64.569 kb on + strand, within Ac3H11_385 at 64.569 kb on + strand, within Ac3H11_385 at 64.569 kb on + strand, within Ac3H11_385 at 64.569 kb on + strand, within Ac3H11_385 at 64.570 kb on - strand, within Ac3H11_385 at 64.570 kb on - strand, within Ac3H11_385 at 64.570 kb on - strand, within Ac3H11_385 at 64.684 kb on + strand, within Ac3H11_385 at 64.749 kb on + strand, within Ac3H11_385 at 64.749 kb on + strand, within Ac3H11_385 at 64.749 kb on + strand, within Ac3H11_385 at 64.750 kb on - strand, within Ac3H11_385 at 64.750 kb on - strand, within Ac3H11_385 at 64.750 kb on - strand, within Ac3H11_385 at 64.790 kb on + strand, within Ac3H11_385 at 64.790 kb on + strand, within Ac3H11_385 at 64.790 kb on + strand, within Ac3H11_385 at 64.791 kb on - strand, within Ac3H11_385 at 64.851 kb on + strand, within Ac3H11_385 at 64.851 kb on + strand, within Ac3H11_385 at 64.852 kb on - strand, within Ac3H11_385 at 64.852 kb on - strand, within Ac3H11_385 at 64.852 kb on - strand, within Ac3H11_385 at 64.852 kb on - strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.914 kb on + strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 64.915 kb on - strand, within Ac3H11_385 at 65.133 kb on - strand at 65.133 kb on - strand at 65.271 kb on + strand at 65.272 kb on - strand at 65.355 kb on - strand, within Ac3H11_386 at 65.396 kb on - strand, within Ac3H11_386 at 65.396 kb on - strand, within Ac3H11_386 at 65.944 kb on + strand, within Ac3H11_386 at 65.944 kb on + strand, within Ac3H11_386 at 66.646 kb on - strand, within Ac3H11_386 at 67.125 kb on + strand at 67.125 kb on + strand at 67.172 kb on + strand at 67.172 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 64,140 - Ac3H11_384 0.76 +0.8 64,140 - Ac3H11_384 0.76 -0.9 64,277 + Ac3H11_384 0.89 -0.0 64,277 + Ac3H11_384 0.89 +0.8 64,277 + Ac3H11_384 0.89 +0.8 64,277 + Ac3H11_384 0.89 +2.0 64,278 - Ac3H11_384 0.90 +2.3 64,278 - Ac3H11_384 0.90 -0.9 64,278 - Ac3H11_384 0.90 -1.3 64,295 + +0.5 64,295 + -0.4 64,296 - -0.3 64,296 - -0.0 64,296 - -0.1 64,412 - -1.0 64,520 + Ac3H11_385 0.10 +0.8 64,520 + Ac3H11_385 0.10 +1.9 64,520 + Ac3H11_385 0.10 -0.9 64,521 - Ac3H11_385 0.10 -1.7 64,569 + Ac3H11_385 0.19 -2.2 64,569 + Ac3H11_385 0.19 +2.1 64,569 + Ac3H11_385 0.19 -0.4 64,569 + Ac3H11_385 0.19 -0.5 64,570 - Ac3H11_385 0.19 +1.5 64,570 - Ac3H11_385 0.19 -0.3 64,570 - Ac3H11_385 0.19 -0.1 64,684 + Ac3H11_385 0.39 -2.0 64,749 + Ac3H11_385 0.51 +0.1 64,749 + Ac3H11_385 0.51 +0.7 64,749 + Ac3H11_385 0.51 +0.7 64,750 - Ac3H11_385 0.51 -1.8 64,750 - Ac3H11_385 0.51 +1.4 64,750 - Ac3H11_385 0.51 +0.6 64,790 + Ac3H11_385 0.58 +0.2 64,790 + Ac3H11_385 0.58 -3.5 64,790 + Ac3H11_385 0.58 +2.5 64,791 - Ac3H11_385 0.58 -2.5 64,851 + Ac3H11_385 0.69 +0.6 64,851 + Ac3H11_385 0.69 +0.2 64,852 - Ac3H11_385 0.69 -0.2 64,852 - Ac3H11_385 0.69 +0.7 64,852 - Ac3H11_385 0.69 +1.5 64,852 - Ac3H11_385 0.69 -0.1 64,914 + Ac3H11_385 0.80 +0.1 64,914 + Ac3H11_385 0.80 +1.7 64,914 + Ac3H11_385 0.80 -0.5 64,914 + Ac3H11_385 0.80 +0.9 64,914 + Ac3H11_385 0.80 -1.5 64,914 + Ac3H11_385 0.80 +1.2 64,914 + Ac3H11_385 0.80 +1.9 64,914 + Ac3H11_385 0.80 -0.2 64,914 + Ac3H11_385 0.80 +1.1 64,914 + Ac3H11_385 0.80 -0.4 64,914 + Ac3H11_385 0.80 +0.6 64,915 - Ac3H11_385 0.81 +1.1 64,915 - Ac3H11_385 0.81 -1.7 64,915 - Ac3H11_385 0.81 +1.6 64,915 - Ac3H11_385 0.81 -0.1 64,915 - Ac3H11_385 0.81 +0.7 64,915 - Ac3H11_385 0.81 -0.3 64,915 - Ac3H11_385 0.81 -0.0 64,915 - Ac3H11_385 0.81 +1.7 64,915 - Ac3H11_385 0.81 +0.7 64,915 - Ac3H11_385 0.81 -1.0 64,915 - Ac3H11_385 0.81 -3.4 64,915 - Ac3H11_385 0.81 -0.8 64,915 - Ac3H11_385 0.81 -2.5 65,133 - -0.1 65,133 - -0.0 65,271 + +1.0 65,272 - +1.5 65,355 - Ac3H11_386 0.13 -1.4 65,396 - Ac3H11_386 0.15 -1.8 65,396 - Ac3H11_386 0.15 +0.8 65,944 + Ac3H11_386 0.45 +1.4 65,944 + Ac3H11_386 0.45 -2.0 66,646 - Ac3H11_386 0.84 -0.2 67,125 + +2.5 67,125 + +1.0 67,172 + -0.6 67,172 + -0.9
Or see this region's nucleotide sequence