Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3396 and Ac3H11_3397 are separated by 44 nucleotides Ac3H11_3397 and Ac3H11_3398 are separated by 54 nucleotides Ac3H11_3398 and Ac3H11_3399 are separated by 48 nucleotides Ac3H11_3399 and Ac3H11_3400 are separated by 32 nucleotides
Ac3H11_3396 - ProQ: influences osmotic activation of compatible solute ProP, at 73,466 to 74,437
_3396
Ac3H11_3397 - Conserved domain protein, at 74,482 to 75,096
_3397
Ac3H11_3398 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 75,151 to 76,404
_3398
Ac3H11_3399 - Probable transmembrane protein, at 76,453 to 77,250
_3399
Ac3H11_3400 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 77,283 to 78,410
_3400
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 74.483 kb on + strand at 74.484 kb on - strand at 74.484 kb on - strand at 74.484 kb on - strand at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.756 kb on + strand, within Ac3H11_3397 at 74.794 kb on + strand, within Ac3H11_3397 at 74.794 kb on + strand, within Ac3H11_3397 at 74.795 kb on - strand, within Ac3H11_3397 at 74.941 kb on + strand, within Ac3H11_3397 at 74.942 kb on - strand, within Ac3H11_3397 at 75.133 kb on - strand at 75.320 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.348 kb on - strand, within Ac3H11_3398 at 75.479 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.605 kb on + strand, within Ac3H11_3398 at 75.605 kb on + strand, within Ac3H11_3398 at 75.635 kb on + strand, within Ac3H11_3398 at 76.133 kb on + strand, within Ac3H11_3398 at 76.134 kb on - strand, within Ac3H11_3398 at 76.134 kb on - strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.248 kb on - strand, within Ac3H11_3398 at 76.248 kb on - strand, within Ac3H11_3398 at 76.292 kb on + strand at 76.293 kb on - strand at 76.293 kb on - strand at 76.293 kb on - strand at 76.293 kb on - strand at 77.044 kb on + strand, within Ac3H11_3399 at 77.044 kb on + strand, within Ac3H11_3399 at 77.044 kb on + strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.284 kb on + strand at 77.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 74,483 + +0.1 74,484 - -2.5 74,484 - +0.0 74,484 - -0.9 74,634 - Ac3H11_3397 0.25 +1.8 74,634 - Ac3H11_3397 0.25 -2.7 74,634 - Ac3H11_3397 0.25 -1.7 74,634 - Ac3H11_3397 0.25 -3.1 74,756 + Ac3H11_3397 0.45 +0.6 74,794 + Ac3H11_3397 0.51 +2.2 74,794 + Ac3H11_3397 0.51 -0.2 74,795 - Ac3H11_3397 0.51 -0.3 74,941 + Ac3H11_3397 0.75 -0.3 74,942 - Ac3H11_3397 0.75 +1.6 75,133 - -0.0 75,320 + Ac3H11_3398 0.13 -0.7 75,347 + Ac3H11_3398 0.16 +0.3 75,347 + Ac3H11_3398 0.16 -1.4 75,347 + Ac3H11_3398 0.16 +1.3 75,347 + Ac3H11_3398 0.16 +0.4 75,347 + Ac3H11_3398 0.16 -0.8 75,347 + Ac3H11_3398 0.16 -2.9 75,348 - Ac3H11_3398 0.16 -1.9 75,479 + Ac3H11_3398 0.26 +0.8 75,485 + Ac3H11_3398 0.27 +0.7 75,485 + Ac3H11_3398 0.27 +0.2 75,485 + Ac3H11_3398 0.27 +1.2 75,485 + Ac3H11_3398 0.27 -1.3 75,485 + Ac3H11_3398 0.27 +1.4 75,485 + Ac3H11_3398 0.27 +0.5 75,485 + Ac3H11_3398 0.27 -1.7 75,485 + Ac3H11_3398 0.27 +2.8 75,485 + Ac3H11_3398 0.27 +1.9 75,485 + Ac3H11_3398 0.27 -1.2 75,486 - Ac3H11_3398 0.27 +1.4 75,486 - Ac3H11_3398 0.27 +0.5 75,486 - Ac3H11_3398 0.27 +0.3 75,486 - Ac3H11_3398 0.27 +3.4 75,486 - Ac3H11_3398 0.27 -2.9 75,486 - Ac3H11_3398 0.27 -1.8 75,486 - Ac3H11_3398 0.27 -2.9 75,486 - Ac3H11_3398 0.27 +1.6 75,486 - Ac3H11_3398 0.27 +2.2 75,486 - Ac3H11_3398 0.27 +0.8 75,486 - Ac3H11_3398 0.27 -1.2 75,486 - Ac3H11_3398 0.27 +0.3 75,486 - Ac3H11_3398 0.27 -0.2 75,605 + Ac3H11_3398 0.36 +0.4 75,605 + Ac3H11_3398 0.36 -0.3 75,635 + Ac3H11_3398 0.39 -1.7 76,133 + Ac3H11_3398 0.78 +0.1 76,134 - Ac3H11_3398 0.78 -1.4 76,134 - Ac3H11_3398 0.78 -2.0 76,247 + Ac3H11_3398 0.87 -0.7 76,247 + Ac3H11_3398 0.87 -1.4 76,247 + Ac3H11_3398 0.87 +0.4 76,247 + Ac3H11_3398 0.87 -1.6 76,248 - Ac3H11_3398 0.87 +0.8 76,248 - Ac3H11_3398 0.87 +2.1 76,292 + -1.3 76,293 - -1.8 76,293 - -0.0 76,293 - -2.2 76,293 - -3.1 77,044 + Ac3H11_3399 0.74 +0.5 77,044 + Ac3H11_3399 0.74 +0.9 77,044 + Ac3H11_3399 0.74 +0.8 77,045 - Ac3H11_3399 0.74 +1.4 77,045 - Ac3H11_3399 0.74 -1.0 77,045 - Ac3H11_3399 0.74 +1.9 77,045 - Ac3H11_3399 0.74 -1.6 77,045 - Ac3H11_3399 0.74 -2.2 77,126 + Ac3H11_3399 0.84 -1.6 77,126 + Ac3H11_3399 0.84 +1.1 77,126 + Ac3H11_3399 0.84 +0.1 77,126 + Ac3H11_3399 0.84 -0.2 77,126 + Ac3H11_3399 0.84 -0.1 77,127 - Ac3H11_3399 0.84 +0.3 77,127 - Ac3H11_3399 0.84 +0.0 77,127 - Ac3H11_3399 0.84 +1.3 77,127 - Ac3H11_3399 0.84 -1.0 77,127 - Ac3H11_3399 0.84 +1.6 77,284 + +3.1 77,284 + +2.3
Or see this region's nucleotide sequence