Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1947 and Ac3H11_1948 are separated by 68 nucleotides Ac3H11_1948 and Ac3H11_1949 are separated by 94 nucleotides Ac3H11_1949 and Ac3H11_1950 are separated by 56 nucleotides
Ac3H11_1947 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 161,789 to 162,721
_1947
Ac3H11_1948 - hypothetical protein, at 162,790 to 163,134
_1948
Ac3H11_1949 - TRAP-type transport system, periplasmic component, predicted N-acetylneuraminate-binding protein, at 163,229 to 164,266
_1949
Ac3H11_1950 - TRAP-type C4-dicarboxylate transport system, large permease component, at 164,323 to 165,621
_1950
Position (kb) 163
164
165 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 162.296 kb on + strand, within Ac3H11_1947 at 162.335 kb on + strand, within Ac3H11_1947 at 162.335 kb on + strand, within Ac3H11_1947 at 162.336 kb on - strand, within Ac3H11_1947 at 162.554 kb on + strand, within Ac3H11_1947 at 162.554 kb on + strand, within Ac3H11_1947 at 162.555 kb on - strand, within Ac3H11_1947 at 162.772 kb on - strand at 162.786 kb on - strand at 163.018 kb on + strand, within Ac3H11_1948 at 163.019 kb on - strand, within Ac3H11_1948 at 163.019 kb on - strand, within Ac3H11_1948 at 163.019 kb on - strand, within Ac3H11_1948 at 163.133 kb on - strand at 163.168 kb on - strand at 163.230 kb on + strand at 163.305 kb on + strand at 163.306 kb on - strand at 163.306 kb on - strand at 163.306 kb on - strand at 163.549 kb on - strand, within Ac3H11_1949 at 163.549 kb on - strand, within Ac3H11_1949 at 163.905 kb on + strand, within Ac3H11_1949 at 164.059 kb on - strand, within Ac3H11_1949 at 164.167 kb on - strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.178 kb on + strand at 164.179 kb on - strand at 164.179 kb on - strand at 164.179 kb on - strand at 164.306 kb on + strand at 164.306 kb on + strand at 164.306 kb on + strand at 164.307 kb on - strand at 164.307 kb on - strand at 164.313 kb on + strand at 164.313 kb on + strand at 164.313 kb on + strand at 164.313 kb on + strand at 164.555 kb on + strand, within Ac3H11_1950 at 164.555 kb on + strand, within Ac3H11_1950 at 164.556 kb on - strand, within Ac3H11_1950 at 164.556 kb on - strand, within Ac3H11_1950 at 164.556 kb on - strand, within Ac3H11_1950 at 164.556 kb on - strand, within Ac3H11_1950 at 164.851 kb on + strand, within Ac3H11_1950 at 164.851 kb on + strand, within Ac3H11_1950 at 164.852 kb on - strand, within Ac3H11_1950 at 164.852 kb on - strand, within Ac3H11_1950 at 164.852 kb on - strand, within Ac3H11_1950 at 164.852 kb on - strand, within Ac3H11_1950 at 164.864 kb on + strand, within Ac3H11_1950 at 164.864 kb on + strand, within Ac3H11_1950 at 164.864 kb on + strand, within Ac3H11_1950 at 164.865 kb on - strand, within Ac3H11_1950 at 165.134 kb on + strand, within Ac3H11_1950 at 165.134 kb on + strand, within Ac3H11_1950 at 165.135 kb on - strand, within Ac3H11_1950
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 162,296 + Ac3H11_1947 0.54 +0.4 162,335 + Ac3H11_1947 0.59 +0.6 162,335 + Ac3H11_1947 0.59 -1.0 162,336 - Ac3H11_1947 0.59 -0.6 162,554 + Ac3H11_1947 0.82 -2.0 162,554 + Ac3H11_1947 0.82 +1.0 162,555 - Ac3H11_1947 0.82 -0.6 162,772 - +0.7 162,786 - +0.2 163,018 + Ac3H11_1948 0.66 +1.0 163,019 - Ac3H11_1948 0.66 -1.1 163,019 - Ac3H11_1948 0.66 +1.7 163,019 - Ac3H11_1948 0.66 -0.1 163,133 - -1.6 163,168 - +1.5 163,230 + +0.4 163,305 + -0.5 163,306 - -1.5 163,306 - -2.0 163,306 - +1.7 163,549 - Ac3H11_1949 0.31 -0.4 163,549 - Ac3H11_1949 0.31 +0.0 163,905 + Ac3H11_1949 0.65 -0.6 164,059 - Ac3H11_1949 0.80 -0.9 164,167 - +0.8 164,178 + +0.2 164,178 + +1.8 164,178 + +1.6 164,178 + -2.5 164,178 + +0.7 164,178 + -0.6 164,178 + +0.8 164,178 + +0.8 164,178 + -2.8 164,179 - -2.5 164,179 - +0.7 164,179 - -0.2 164,306 + -1.6 164,306 + -1.9 164,306 + +0.9 164,307 - -1.6 164,307 - -0.4 164,313 + -1.1 164,313 + +3.8 164,313 + -0.5 164,313 + -3.8 164,555 + Ac3H11_1950 0.18 +0.6 164,555 + Ac3H11_1950 0.18 +1.7 164,556 - Ac3H11_1950 0.18 +0.6 164,556 - Ac3H11_1950 0.18 +1.4 164,556 - Ac3H11_1950 0.18 +2.9 164,556 - Ac3H11_1950 0.18 +0.5 164,851 + Ac3H11_1950 0.41 +1.5 164,851 + Ac3H11_1950 0.41 +1.8 164,852 - Ac3H11_1950 0.41 +0.2 164,852 - Ac3H11_1950 0.41 +1.1 164,852 - Ac3H11_1950 0.41 +1.6 164,852 - Ac3H11_1950 0.41 -1.9 164,864 + Ac3H11_1950 0.42 -3.2 164,864 + Ac3H11_1950 0.42 -0.9 164,864 + Ac3H11_1950 0.42 +2.0 164,865 - Ac3H11_1950 0.42 +0.5 165,134 + Ac3H11_1950 0.62 -0.4 165,134 + Ac3H11_1950 0.62 -1.8 165,135 - Ac3H11_1950 0.63 +0.6
Or see this region's nucleotide sequence