Experiment: Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23560 and AO353_23565 are separated by 14 nucleotides AO353_23565 and AO353_23570 overlap by 35 nucleotides AO353_23570 and AO353_23575 are separated by 89 nucleotides AO353_23575 and AO353_23580 are separated by 184 nucleotides
AO353_23560: AO353_23560 - lysine decarboxylase, at 5,159,218 to 5,161,473
_23560
AO353_23565: AO353_23565 - GNAT family acetyltransferase, at 5,161,488 to 5,162,102
_23565
AO353_23570: AO353_23570 - LysR family transcriptional regulator, at 5,162,068 to 5,162,961
_23570
AO353_23575: AO353_23575 - hypothetical protein, at 5,163,051 to 5,163,287
_23575
AO353_23580: AO353_23580 - ACP phosphodiesterase, at 5,163,472 to 5,164,029
_23580
Position (kb) 5162
5163 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5161.146 kb on + strand, within AO353_23560 at 5161.146 kb on + strand, within AO353_23560 at 5161.147 kb on - strand, within AO353_23560 at 5161.156 kb on + strand, within AO353_23560 at 5161.156 kb on + strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.157 kb on - strand, within AO353_23560 at 5161.351 kb on + strand at 5161.447 kb on + strand at 5161.447 kb on + strand at 5161.447 kb on + strand at 5161.447 kb on + strand at 5161.447 kb on + strand at 5161.448 kb on - strand at 5161.448 kb on - strand at 5161.487 kb on + strand at 5161.488 kb on - strand at 5161.557 kb on + strand, within AO353_23565 at 5161.557 kb on + strand, within AO353_23565 at 5161.557 kb on + strand, within AO353_23565 at 5161.557 kb on + strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.558 kb on - strand, within AO353_23565 at 5161.559 kb on + strand, within AO353_23565 at 5161.559 kb on + strand, within AO353_23565 at 5161.559 kb on + strand, within AO353_23565 at 5161.559 kb on + strand, within AO353_23565 at 5161.559 kb on + strand, within AO353_23565 at 5161.560 kb on - strand, within AO353_23565 at 5161.560 kb on - strand, within AO353_23565 at 5161.560 kb on - strand, within AO353_23565 at 5161.560 kb on - strand, within AO353_23565 at 5161.560 kb on - strand, within AO353_23565 at 5161.775 kb on + strand, within AO353_23565 at 5161.919 kb on + strand, within AO353_23565 at 5161.920 kb on - strand, within AO353_23565 at 5161.944 kb on + strand, within AO353_23565 at 5161.959 kb on + strand, within AO353_23565 at 5161.959 kb on + strand, within AO353_23565 at 5161.959 kb on + strand, within AO353_23565 at 5162.103 kb on + strand at 5162.104 kb on - strand at 5162.162 kb on + strand, within AO353_23570 at 5162.233 kb on - strand, within AO353_23570 at 5162.700 kb on - strand, within AO353_23570 at 5162.700 kb on - strand, within AO353_23570 at 5162.707 kb on + strand, within AO353_23570 at 5162.707 kb on + strand, within AO353_23570 at 5162.748 kb on - strand, within AO353_23570 at 5162.748 kb on - strand, within AO353_23570 at 5162.953 kb on + strand at 5162.954 kb on - strand at 5162.954 kb on - strand at 5163.027 kb on - strand at 5163.027 kb on - strand at 5163.027 kb on - strand at 5163.041 kb on + strand at 5163.053 kb on - strand at 5163.109 kb on - strand, within AO353_23575 at 5163.109 kb on - strand, within AO353_23575 at 5163.158 kb on + strand, within AO353_23575 at 5163.158 kb on + strand, within AO353_23575 at 5163.158 kb on + strand, within AO353_23575 at 5163.159 kb on - strand, within AO353_23575 at 5163.159 kb on - strand, within AO353_23575 at 5163.159 kb on - strand, within AO353_23575 at 5163.160 kb on + strand, within AO353_23575 at 5163.160 kb on + strand, within AO353_23575 at 5163.161 kb on - strand, within AO353_23575 at 5163.161 kb on - strand, within AO353_23575 at 5163.161 kb on - strand, within AO353_23575 at 5163.161 kb on - strand, within AO353_23575 at 5163.161 kb on - strand, within AO353_23575 at 5163.233 kb on - strand, within AO353_23575 at 5163.276 kb on + strand at 5163.276 kb on + strand at 5163.277 kb on - strand at 5163.294 kb on - strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.308 kb on + strand at 5163.309 kb on - strand at 5163.344 kb on - strand at 5163.352 kb on + strand at 5163.353 kb on - strand at 5163.353 kb on - strand at 5163.362 kb on + strand at 5163.378 kb on + strand at 5163.378 kb on + strand at 5163.378 kb on + strand at 5163.379 kb on - strand at 5163.379 kb on - strand at 5163.390 kb on - strand at 5163.390 kb on - strand at 5163.411 kb on + strand at 5163.411 kb on + strand at 5163.412 kb on - strand at 5163.412 kb on - strand at 5163.412 kb on - strand at 5163.412 kb on - strand at 5163.430 kb on + strand at 5163.430 kb on + strand at 5163.430 kb on + strand at 5163.430 kb on + strand at 5163.430 kb on + strand at 5163.430 kb on + strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.431 kb on - strand at 5163.432 kb on + strand at 5163.432 kb on + strand at 5163.432 kb on + strand at 5163.432 kb on + strand at 5163.432 kb on + strand at 5163.433 kb on - strand at 5163.433 kb on - strand at 5163.433 kb on - strand at 5163.433 kb on - strand at 5163.436 kb on + strand at 5163.436 kb on + strand at 5163.436 kb on + strand at 5163.436 kb on + strand at 5163.437 kb on - strand at 5163.457 kb on - strand at 5163.486 kb on + strand at 5163.487 kb on - strand at 5163.614 kb on - strand, within AO353_23580 at 5163.614 kb on - strand, within AO353_23580 at 5163.614 kb on - strand, within AO353_23580 at 5163.632 kb on + strand, within AO353_23580 at 5163.633 kb on - strand, within AO353_23580 at 5163.633 kb on - strand, within AO353_23580 at 5163.633 kb on - strand, within AO353_23580 at 5163.649 kb on + strand, within AO353_23580 at 5163.649 kb on + strand, within AO353_23580 at 5163.650 kb on - strand, within AO353_23580 at 5163.650 kb on - strand, within AO353_23580 at 5163.650 kb on - strand, within AO353_23580 at 5163.710 kb on - strand, within AO353_23580 at 5163.710 kb on - strand, within AO353_23580 at 5163.710 kb on - strand, within AO353_23580 at 5163.711 kb on + strand, within AO353_23580 at 5163.712 kb on - strand, within AO353_23580 at 5163.843 kb on + strand, within AO353_23580 at 5163.907 kb on + strand, within AO353_23580 at 5163.907 kb on + strand, within AO353_23580 at 5163.908 kb on - strand, within AO353_23580 at 5163.908 kb on - strand, within AO353_23580
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0006 mg/ml remove 5,161,146 + AO353_23560 0.85 +3.0 5,161,146 + AO353_23560 0.85 -2.6 5,161,147 - AO353_23560 0.86 -1.0 5,161,156 + AO353_23560 0.86 -1.0 5,161,156 + AO353_23560 0.86 +2.2 5,161,157 - AO353_23560 0.86 -2.5 5,161,157 - AO353_23560 0.86 -0.5 5,161,157 - AO353_23560 0.86 +0.9 5,161,157 - AO353_23560 0.86 +0.2 5,161,157 - AO353_23560 0.86 +1.0 5,161,157 - AO353_23560 0.86 -1.9 5,161,351 + -2.2 5,161,447 + -1.5 5,161,447 + -2.0 5,161,447 + +0.5 5,161,447 + +1.0 5,161,447 + -1.7 5,161,448 - +2.2 5,161,448 - -2.1 5,161,487 + -0.8 5,161,488 - +0.5 5,161,557 + AO353_23565 0.11 -1.1 5,161,557 + AO353_23565 0.11 -1.9 5,161,557 + AO353_23565 0.11 +0.9 5,161,557 + AO353_23565 0.11 -0.9 5,161,558 - AO353_23565 0.11 +0.5 5,161,558 - AO353_23565 0.11 -1.5 5,161,558 - AO353_23565 0.11 +1.0 5,161,558 - AO353_23565 0.11 +1.5 5,161,558 - AO353_23565 0.11 +0.9 5,161,558 - AO353_23565 0.11 +1.2 5,161,558 - AO353_23565 0.11 -0.9 5,161,558 - AO353_23565 0.11 -0.1 5,161,558 - AO353_23565 0.11 +0.7 5,161,559 + AO353_23565 0.12 -1.5 5,161,559 + AO353_23565 0.12 +0.6 5,161,559 + AO353_23565 0.12 -0.9 5,161,559 + AO353_23565 0.12 -0.1 5,161,559 + AO353_23565 0.12 -1.4 5,161,560 - AO353_23565 0.12 +0.5 5,161,560 - AO353_23565 0.12 -1.1 5,161,560 - AO353_23565 0.12 +0.5 5,161,560 - AO353_23565 0.12 -0.5 5,161,560 - AO353_23565 0.12 -1.4 5,161,775 + AO353_23565 0.47 +1.7 5,161,919 + AO353_23565 0.70 -0.9 5,161,920 - AO353_23565 0.70 -0.1 5,161,944 + AO353_23565 0.74 -0.9 5,161,959 + AO353_23565 0.77 +1.0 5,161,959 + AO353_23565 0.77 +3.9 5,161,959 + AO353_23565 0.77 -0.5 5,162,103 + +0.8 5,162,104 - -1.1 5,162,162 + AO353_23570 0.11 -2.4 5,162,233 - AO353_23570 0.18 +1.1 5,162,700 - AO353_23570 0.71 -1.7 5,162,700 - AO353_23570 0.71 +1.5 5,162,707 + AO353_23570 0.71 +0.6 5,162,707 + AO353_23570 0.71 +3.1 5,162,748 - AO353_23570 0.76 +1.5 5,162,748 - AO353_23570 0.76 -2.0 5,162,953 + -2.0 5,162,954 - +1.8 5,162,954 - +0.5 5,163,027 - +0.9 5,163,027 - +1.8 5,163,027 - -1.4 5,163,041 + -1.5 5,163,053 - -2.0 5,163,109 - AO353_23575 0.24 -0.5 5,163,109 - AO353_23575 0.24 -1.1 5,163,158 + AO353_23575 0.45 -2.1 5,163,158 + AO353_23575 0.45 -2.5 5,163,158 + AO353_23575 0.45 +0.1 5,163,159 - AO353_23575 0.46 -1.5 5,163,159 - AO353_23575 0.46 -0.5 5,163,159 - AO353_23575 0.46 -0.9 5,163,160 + AO353_23575 0.46 -0.7 5,163,160 + AO353_23575 0.46 -1.5 5,163,161 - AO353_23575 0.46 -1.4 5,163,161 - AO353_23575 0.46 -1.4 5,163,161 - AO353_23575 0.46 +1.0 5,163,161 - AO353_23575 0.46 -0.5 5,163,161 - AO353_23575 0.46 -1.7 5,163,233 - AO353_23575 0.77 +2.0 5,163,276 + -0.5 5,163,276 + -1.0 5,163,277 - -1.1 5,163,294 - -1.1 5,163,308 + +0.9 5,163,308 + -0.9 5,163,308 + -0.5 5,163,308 + -1.2 5,163,308 + -2.0 5,163,308 + +0.1 5,163,308 + +3.0 5,163,309 - -0.5 5,163,344 - -0.1 5,163,352 + -0.5 5,163,353 - +0.9 5,163,353 - -0.3 5,163,362 + -0.1 5,163,378 + -2.2 5,163,378 + -1.4 5,163,378 + +2.2 5,163,379 - +0.1 5,163,379 - -0.9 5,163,390 - -2.2 5,163,390 - -0.5 5,163,411 + +0.5 5,163,411 + +1.0 5,163,412 - -0.5 5,163,412 - -2.7 5,163,412 - -0.9 5,163,412 - +0.5 5,163,430 + +0.9 5,163,430 + +0.5 5,163,430 + -0.1 5,163,430 + -1.4 5,163,430 + +2.2 5,163,430 + +1.3 5,163,431 - -0.9 5,163,431 - -0.1 5,163,431 - -0.1 5,163,431 - -1.5 5,163,431 - +2.2 5,163,431 - +1.2 5,163,431 - -2.1 5,163,431 - +0.5 5,163,431 - +1.1 5,163,432 + +0.5 5,163,432 + -0.1 5,163,432 + -1.7 5,163,432 + -1.4 5,163,432 + -0.1 5,163,433 - -0.1 5,163,433 - +3.3 5,163,433 - -0.1 5,163,433 - +0.9 5,163,436 + +1.5 5,163,436 + -0.5 5,163,436 + -2.0 5,163,436 + -2.6 5,163,437 - -0.2 5,163,457 - -1.7 5,163,486 + -0.9 5,163,487 - -0.9 5,163,614 - AO353_23580 0.25 +3.2 5,163,614 - AO353_23580 0.25 -2.0 5,163,614 - AO353_23580 0.25 +2.6 5,163,632 + AO353_23580 0.29 -2.4 5,163,633 - AO353_23580 0.29 +0.7 5,163,633 - AO353_23580 0.29 -0.1 5,163,633 - AO353_23580 0.29 +2.3 5,163,649 + AO353_23580 0.32 -1.1 5,163,649 + AO353_23580 0.32 +0.5 5,163,650 - AO353_23580 0.32 -0.7 5,163,650 - AO353_23580 0.32 +1.0 5,163,650 - AO353_23580 0.32 -0.1 5,163,710 - AO353_23580 0.43 +0.5 5,163,710 - AO353_23580 0.43 +0.3 5,163,710 - AO353_23580 0.43 -0.5 5,163,711 + AO353_23580 0.43 +1.3 5,163,712 - AO353_23580 0.43 +0.5 5,163,843 + AO353_23580 0.66 -0.0 5,163,907 + AO353_23580 0.78 -0.9 5,163,907 + AO353_23580 0.78 -2.1 5,163,908 - AO353_23580 0.78 -0.3 5,163,908 - AO353_23580 0.78 +0.5
Or see this region's nucleotide sequence