Experiment: Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_20835 and AO353_20840 overlap by 4 nucleotides AO353_20840 and AO353_20845 are separated by 45 nucleotides AO353_20845 and AO353_20850 are separated by 41 nucleotides AO353_20850 and AO353_20855 are separated by 128 nucleotides AO353_20855 and AO353_20860 are separated by 163 nucleotides
AO353_20835: AO353_20835 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 4,562,793 to 4,563,710
_20835
AO353_20840: AO353_20840 - ribose pyranase, at 4,563,707 to 4,564,111
_20840
AO353_20845: AO353_20845 - uridine/adenosine nucleosidase (EC 3.2.2.3; EC 3.2.2.7) <small>(from data)</small>, at 4,564,157 to 4,565,185
_20845
AO353_20850: AO353_20850 - hypothetical protein, at 4,565,227 to 4,565,430
_20850
AO353_20855: AO353_20855 - hypothetical protein, at 4,565,559 to 4,565,864
_20855
AO353_20860: AO353_20860 - hypothetical protein, at 4,566,028 to 4,566,342
_20860
Position (kb) 4564
4565
4566 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4563.234 kb on + strand, within AO353_20835 at 4563.234 kb on + strand, within AO353_20835 at 4563.234 kb on + strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.235 kb on - strand, within AO353_20835 at 4563.333 kb on + strand, within AO353_20835 at 4563.333 kb on + strand, within AO353_20835 at 4563.333 kb on + strand, within AO353_20835 at 4563.688 kb on + strand at 4563.689 kb on - strand at 4563.689 kb on - strand at 4563.689 kb on - strand at 4563.865 kb on + strand, within AO353_20840 at 4563.865 kb on + strand, within AO353_20840 at 4563.865 kb on + strand, within AO353_20840 at 4564.018 kb on + strand, within AO353_20840 at 4564.024 kb on - strand, within AO353_20840 at 4564.024 kb on - strand, within AO353_20840 at 4564.074 kb on - strand at 4564.074 kb on - strand at 4564.109 kb on + strand at 4564.110 kb on - strand at 4564.110 kb on - strand at 4564.110 kb on - strand at 4564.135 kb on - strand at 4564.156 kb on + strand at 4564.156 kb on + strand at 4564.156 kb on + strand at 4564.156 kb on + strand at 4564.157 kb on - strand at 4564.157 kb on - strand at 4564.157 kb on - strand at 4564.157 kb on - strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.160 kb on + strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.161 kb on - strand at 4564.225 kb on + strand at 4564.252 kb on - strand at 4564.252 kb on - strand at 4564.252 kb on - strand at 4564.348 kb on + strand, within AO353_20845 at 4564.436 kb on + strand, within AO353_20845 at 4564.436 kb on + strand, within AO353_20845 at 4564.437 kb on - strand, within AO353_20845 at 4564.475 kb on + strand, within AO353_20845 at 4564.475 kb on + strand, within AO353_20845 at 4564.475 kb on + strand, within AO353_20845 at 4564.475 kb on + strand, within AO353_20845 at 4564.475 kb on + strand, within AO353_20845 at 4564.476 kb on - strand, within AO353_20845 at 4564.476 kb on - strand, within AO353_20845 at 4564.566 kb on - strand, within AO353_20845 at 4564.808 kb on + strand, within AO353_20845 at 4564.808 kb on + strand, within AO353_20845 at 4564.809 kb on - strand, within AO353_20845 at 4564.809 kb on - strand, within AO353_20845 at 4564.907 kb on - strand, within AO353_20845 at 4564.919 kb on + strand, within AO353_20845 at 4564.919 kb on + strand, within AO353_20845 at 4564.920 kb on - strand, within AO353_20845 at 4564.920 kb on - strand, within AO353_20845 at 4564.944 kb on - strand, within AO353_20845 at 4564.944 kb on - strand, within AO353_20845 at 4564.944 kb on - strand, within AO353_20845 at 4565.084 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.183 kb on + strand at 4565.184 kb on - strand at 4565.184 kb on - strand at 4565.184 kb on - strand at 4565.184 kb on - strand at 4565.219 kb on + strand at 4565.219 kb on + strand at 4565.220 kb on - strand at 4565.228 kb on + strand at 4565.228 kb on + strand at 4565.228 kb on + strand at 4565.228 kb on + strand at 4565.228 kb on + strand at 4565.229 kb on - strand at 4565.229 kb on - strand at 4565.229 kb on - strand at 4565.229 kb on - strand at 4565.229 kb on - strand at 4565.229 kb on - strand at 4565.261 kb on + strand, within AO353_20850 at 4565.261 kb on + strand, within AO353_20850 at 4565.261 kb on + strand, within AO353_20850 at 4565.261 kb on + strand, within AO353_20850 at 4565.262 kb on - strand, within AO353_20850 at 4565.262 kb on - strand, within AO353_20850 at 4565.262 kb on - strand, within AO353_20850 at 4565.272 kb on + strand, within AO353_20850 at 4565.272 kb on + strand, within AO353_20850 at 4565.272 kb on + strand, within AO353_20850 at 4565.272 kb on + strand, within AO353_20850 at 4565.272 kb on + strand, within AO353_20850 at 4565.273 kb on - strand, within AO353_20850 at 4565.329 kb on + strand, within AO353_20850 at 4565.329 kb on + strand, within AO353_20850 at 4565.329 kb on + strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.330 kb on - strand, within AO353_20850 at 4565.369 kb on + strand, within AO353_20850 at 4565.370 kb on - strand, within AO353_20850 at 4565.370 kb on - strand, within AO353_20850 at 4565.370 kb on - strand, within AO353_20850 at 4565.370 kb on - strand, within AO353_20850 at 4565.370 kb on - strand at 4565.469 kb on - strand at 4565.501 kb on + strand at 4565.501 kb on + strand at 4565.501 kb on + strand at 4565.501 kb on + strand at 4565.502 kb on - strand at 4565.502 kb on - strand at 4565.502 kb on - strand at 4565.502 kb on - strand at 4565.502 kb on - strand at 4565.502 kb on - strand at 4565.519 kb on + strand at 4565.520 kb on - strand at 4565.539 kb on + strand at 4565.540 kb on - strand at 4565.577 kb on - strand at 4565.597 kb on - strand, within AO353_20855 at 4565.597 kb on - strand, within AO353_20855 at 4565.597 kb on - strand, within AO353_20855 at 4565.636 kb on + strand, within AO353_20855 at 4565.757 kb on + strand, within AO353_20855 at 4565.758 kb on - strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.761 kb on + strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.762 kb on - strand, within AO353_20855 at 4565.803 kb on + strand, within AO353_20855 at 4565.803 kb on + strand, within AO353_20855 at 4565.804 kb on - strand, within AO353_20855 at 4565.804 kb on - strand, within AO353_20855 at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.847 kb on + strand at 4565.848 kb on - strand at 4565.848 kb on - strand at 4565.848 kb on - strand at 4565.848 kb on - strand at 4565.848 kb on - strand at 4565.855 kb on + strand at 4565.855 kb on + strand at 4565.855 kb on + strand at 4565.859 kb on + strand at 4565.859 kb on + strand at 4565.859 kb on + strand at 4565.859 kb on + strand at 4565.859 kb on + strand at 4565.859 kb on + strand at 4565.860 kb on - strand at 4565.860 kb on - strand at 4565.860 kb on - strand at 4565.860 kb on - strand at 4565.860 kb on - strand at 4565.875 kb on - strand at 4565.875 kb on - strand at 4565.875 kb on - strand at 4565.909 kb on + strand at 4565.909 kb on + strand at 4565.909 kb on + strand at 4565.909 kb on + strand at 4565.910 kb on - strand at 4566.010 kb on + strand at 4566.010 kb on + strand at 4566.011 kb on - strand at 4566.054 kb on + strand at 4566.093 kb on + strand, within AO353_20860 at 4566.093 kb on + strand, within AO353_20860 at 4566.094 kb on - strand, within AO353_20860 at 4566.122 kb on + strand, within AO353_20860
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0006 mg/ml remove 4,563,234 + AO353_20835 0.48 +2.0 4,563,234 + AO353_20835 0.48 -2.2 4,563,234 + AO353_20835 0.48 +2.0 4,563,235 - AO353_20835 0.48 -1.4 4,563,235 - AO353_20835 0.48 -2.6 4,563,235 - AO353_20835 0.48 +0.0 4,563,235 - AO353_20835 0.48 -0.7 4,563,235 - AO353_20835 0.48 -0.4 4,563,235 - AO353_20835 0.48 +0.2 4,563,333 + AO353_20835 0.59 -1.7 4,563,333 + AO353_20835 0.59 +0.6 4,563,333 + AO353_20835 0.59 -2.8 4,563,688 + +0.6 4,563,689 - -0.4 4,563,689 - -0.4 4,563,689 - -0.4 4,563,865 + AO353_20840 0.39 +0.2 4,563,865 + AO353_20840 0.39 +0.4 4,563,865 + AO353_20840 0.39 -0.7 4,564,018 + AO353_20840 0.77 -0.4 4,564,024 - AO353_20840 0.78 +0.3 4,564,024 - AO353_20840 0.78 +0.3 4,564,074 - -1.2 4,564,074 - +0.3 4,564,109 + -0.6 4,564,110 - -2.2 4,564,110 - -1.4 4,564,110 - +2.8 4,564,135 - -0.4 4,564,156 + +1.0 4,564,156 + +2.6 4,564,156 + +0.6 4,564,156 + -1.5 4,564,157 - -2.2 4,564,157 - +0.0 4,564,157 - -1.7 4,564,157 - -0.4 4,564,160 + +1.9 4,564,160 + +0.9 4,564,160 + -2.5 4,564,160 + -1.4 4,564,160 + +1.9 4,564,160 + +1.4 4,564,160 + -0.7 4,564,160 + -0.7 4,564,160 + +0.0 4,564,160 + -2.1 4,564,161 - -0.4 4,564,161 - -0.4 4,564,161 - +1.6 4,564,161 - +0.7 4,564,161 - -1.4 4,564,161 - +0.7 4,564,161 - -2.1 4,564,161 - -1.0 4,564,225 + +0.6 4,564,252 - -1.8 4,564,252 - -1.7 4,564,252 - +2.2 4,564,348 + AO353_20845 0.19 -1.4 4,564,436 + AO353_20845 0.27 +2.2 4,564,436 + AO353_20845 0.27 +0.4 4,564,437 - AO353_20845 0.27 +1.6 4,564,475 + AO353_20845 0.31 -1.8 4,564,475 + AO353_20845 0.31 -0.7 4,564,475 + AO353_20845 0.31 +2.6 4,564,475 + AO353_20845 0.31 -0.2 4,564,475 + AO353_20845 0.31 -1.4 4,564,476 - AO353_20845 0.31 -1.5 4,564,476 - AO353_20845 0.31 -1.0 4,564,566 - AO353_20845 0.40 -0.4 4,564,808 + AO353_20845 0.63 +0.0 4,564,808 + AO353_20845 0.63 -0.4 4,564,809 - AO353_20845 0.63 -1.5 4,564,809 - AO353_20845 0.63 +2.0 4,564,907 - AO353_20845 0.73 -1.0 4,564,919 + AO353_20845 0.74 -2.3 4,564,919 + AO353_20845 0.74 -0.3 4,564,920 - AO353_20845 0.74 -1.5 4,564,920 - AO353_20845 0.74 +0.0 4,564,944 - AO353_20845 0.76 +0.0 4,564,944 - AO353_20845 0.76 +0.1 4,564,944 - AO353_20845 0.76 -0.7 4,565,084 + +0.3 4,565,183 + +2.9 4,565,183 + -1.5 4,565,183 + -1.4 4,565,183 + -1.0 4,565,183 + -0.2 4,565,183 + -0.4 4,565,183 + -1.5 4,565,183 + +2.1 4,565,184 - -0.7 4,565,184 - -0.8 4,565,184 - +1.6 4,565,184 - +2.3 4,565,219 + +0.6 4,565,219 + +1.9 4,565,220 - -1.5 4,565,228 + -0.7 4,565,228 + -0.4 4,565,228 + -1.4 4,565,228 + -0.5 4,565,228 + +0.0 4,565,229 - -0.4 4,565,229 - +2.3 4,565,229 - -1.8 4,565,229 - -0.4 4,565,229 - +1.2 4,565,229 - +3.0 4,565,261 + AO353_20850 0.17 -0.4 4,565,261 + AO353_20850 0.17 -1.8 4,565,261 + AO353_20850 0.17 -2.2 4,565,261 + AO353_20850 0.17 -0.1 4,565,262 - AO353_20850 0.17 +4.1 4,565,262 - AO353_20850 0.17 -1.0 4,565,262 - AO353_20850 0.17 -1.0 4,565,272 + AO353_20850 0.22 -0.7 4,565,272 + AO353_20850 0.22 -0.7 4,565,272 + AO353_20850 0.22 -1.0 4,565,272 + AO353_20850 0.22 +1.4 4,565,272 + AO353_20850 0.22 -0.7 4,565,273 - AO353_20850 0.23 -1.7 4,565,329 + AO353_20850 0.50 -0.4 4,565,329 + AO353_20850 0.50 -1.7 4,565,329 + AO353_20850 0.50 -1.2 4,565,330 - AO353_20850 0.50 -0.4 4,565,330 - AO353_20850 0.50 +0.0 4,565,330 - AO353_20850 0.50 +1.2 4,565,330 - AO353_20850 0.50 -3.0 4,565,330 - AO353_20850 0.50 +0.6 4,565,330 - AO353_20850 0.50 +1.9 4,565,330 - AO353_20850 0.50 -0.4 4,565,330 - AO353_20850 0.50 -0.4 4,565,330 - AO353_20850 0.50 +0.6 4,565,330 - AO353_20850 0.50 +0.3 4,565,369 + AO353_20850 0.70 +0.9 4,565,370 - AO353_20850 0.70 +0.2 4,565,370 - AO353_20850 0.70 +0.0 4,565,370 - AO353_20850 0.70 +0.6 4,565,370 - AO353_20850 0.70 -1.0 4,565,370 - +0.0 4,565,469 - -0.7 4,565,501 + +0.6 4,565,501 + -0.4 4,565,501 + -0.4 4,565,501 + -0.5 4,565,502 - -1.2 4,565,502 - +0.8 4,565,502 - +2.0 4,565,502 - +4.2 4,565,502 - -0.0 4,565,502 - -1.0 4,565,519 + -1.3 4,565,520 - -2.3 4,565,539 + -0.5 4,565,540 - +3.2 4,565,577 - -0.4 4,565,597 - AO353_20855 0.12 +1.6 4,565,597 - AO353_20855 0.12 -0.8 4,565,597 - AO353_20855 0.12 +1.6 4,565,636 + AO353_20855 0.25 -1.7 4,565,757 + AO353_20855 0.65 -0.4 4,565,758 - AO353_20855 0.65 -1.0 4,565,761 + AO353_20855 0.66 -1.5 4,565,761 + AO353_20855 0.66 +0.3 4,565,761 + AO353_20855 0.66 +0.5 4,565,761 + AO353_20855 0.66 -0.7 4,565,761 + AO353_20855 0.66 +0.0 4,565,761 + AO353_20855 0.66 -1.7 4,565,761 + AO353_20855 0.66 -1.2 4,565,761 + AO353_20855 0.66 -0.7 4,565,761 + AO353_20855 0.66 -2.8 4,565,761 + AO353_20855 0.66 -0.8 4,565,761 + AO353_20855 0.66 -1.0 4,565,761 + AO353_20855 0.66 -0.7 4,565,761 + AO353_20855 0.66 +0.0 4,565,761 + AO353_20855 0.66 -1.5 4,565,761 + AO353_20855 0.66 -2.5 4,565,762 - AO353_20855 0.66 +2.4 4,565,762 - AO353_20855 0.66 -1.0 4,565,762 - AO353_20855 0.66 +1.6 4,565,762 - AO353_20855 0.66 +0.6 4,565,762 - AO353_20855 0.66 +2.2 4,565,762 - AO353_20855 0.66 +2.8 4,565,762 - AO353_20855 0.66 -2.4 4,565,762 - AO353_20855 0.66 +3.4 4,565,762 - AO353_20855 0.66 +1.9 4,565,762 - AO353_20855 0.66 +1.6 4,565,762 - AO353_20855 0.66 +0.0 4,565,762 - AO353_20855 0.66 +0.9 4,565,762 - AO353_20855 0.66 +1.5 4,565,762 - AO353_20855 0.66 -1.0 4,565,762 - AO353_20855 0.66 +1.8 4,565,762 - AO353_20855 0.66 +0.9 4,565,762 - AO353_20855 0.66 -1.7 4,565,762 - AO353_20855 0.66 -1.2 4,565,803 + AO353_20855 0.80 -1.0 4,565,803 + AO353_20855 0.80 -2.2 4,565,804 - AO353_20855 0.80 -0.4 4,565,804 - AO353_20855 0.80 +2.6 4,565,847 + -1.5 4,565,847 + -2.0 4,565,847 + +0.6 4,565,847 + -0.4 4,565,847 + -1.7 4,565,847 + -1.8 4,565,847 + -1.2 4,565,848 - -2.9 4,565,848 - -1.5 4,565,848 - +0.7 4,565,848 - +2.2 4,565,848 - +1.1 4,565,855 + -1.5 4,565,855 + -1.4 4,565,855 + -0.7 4,565,859 + -1.0 4,565,859 + -1.0 4,565,859 + -1.5 4,565,859 + -1.2 4,565,859 + -2.3 4,565,859 + +0.0 4,565,860 - -1.5 4,565,860 - +2.5 4,565,860 - -0.7 4,565,860 - +3.7 4,565,860 - +3.3 4,565,875 - -0.4 4,565,875 - +2.3 4,565,875 - +3.8 4,565,909 + -1.0 4,565,909 + -1.5 4,565,909 + +0.6 4,565,909 + -0.4 4,565,910 - +1.5 4,566,010 + -1.5 4,566,010 + -0.4 4,566,011 - -1.2 4,566,054 + +2.6 4,566,093 + AO353_20860 0.21 -1.2 4,566,093 + AO353_20860 0.21 +1.4 4,566,094 - AO353_20860 0.21 +1.4 4,566,122 + AO353_20860 0.30 +3.3
Or see this region's nucleotide sequence