Experiment: Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_08115 and AO353_08120 are separated by 240 nucleotides AO353_08120 and AO353_08125 overlap by 1 nucleotides AO353_08125 and AO353_08130 are separated by 95 nucleotides AO353_08130 and AO353_08135 are separated by 26 nucleotides
AO353_08115: AO353_08115 - energy transducer TonB, at 1,791,856 to 1,792,755
_08115
AO353_08120: AO353_08120 - hypothetical protein, at 1,792,996 to 1,793,565
_08120
AO353_08125: AO353_08125 - Holliday junction resolvase, at 1,793,565 to 1,794,002
_08125
AO353_08130: AO353_08130 - uracil phosphoribosyltransferase, at 1,794,098 to 1,794,604
_08130
AO353_08135: AO353_08135 - aspartate carbamoyltransferase catalytic subunit, at 1,794,631 to 1,795,635
_08135
Position (kb)
1793
1794
1795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1792.579 kb on + strand, within AO353_08115 at 1792.580 kb on - strand, within AO353_08115 at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.749 kb on + strand at 1792.868 kb on - strand at 1792.868 kb on - strand at 1792.868 kb on - strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.017 kb on + strand at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.086 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1793.281 kb on + strand, within AO353_08120 at 1794.043 kb on + strand at 1794.043 kb on + strand at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.243 kb on + strand, within AO353_08130 at 1794.284 kb on + strand, within AO353_08130 at 1794.284 kb on + strand, within AO353_08130
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0006 mg/ml remove 1,792,579 + AO353_08115 0.80 -0.1 1,792,580 - AO353_08115 0.80 -0.0 1,792,749 + +2.4 1,792,749 + -0.8 1,792,749 + +0.5 1,792,749 + -1.0 1,792,868 - -0.7 1,792,868 - -1.4 1,792,868 - +1.9 1,793,017 + +0.2 1,793,017 + -0.4 1,793,017 + +0.2 1,793,017 + -0.4 1,793,086 + AO353_08120 0.16 +0.2 1,793,086 + AO353_08120 0.16 -2.0 1,793,086 + AO353_08120 0.16 -0.1 1,793,086 + AO353_08120 0.16 +0.4 1,793,086 + AO353_08120 0.16 -1.4 1,793,086 + AO353_08120 0.16 +0.6 1,793,086 + AO353_08120 0.16 -0.0 1,793,086 + AO353_08120 0.16 -0.4 1,793,086 + AO353_08120 0.16 -1.1 1,793,281 + AO353_08120 0.50 -2.0 1,793,281 + AO353_08120 0.50 -0.4 1,793,281 + AO353_08120 0.50 -1.4 1,794,043 + -1.5 1,794,043 + -0.8 1,794,243 + AO353_08130 0.29 -0.4 1,794,243 + AO353_08130 0.29 -0.8 1,794,243 + AO353_08130 0.29 +1.2 1,794,243 + AO353_08130 0.29 -2.2 1,794,243 + AO353_08130 0.29 +0.6 1,794,284 + AO353_08130 0.37 -1.1 1,794,284 + AO353_08130 0.37 -0.1
Or see this region's nucleotide sequence