Experiment: Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03810 and AO353_03815 are separated by 449 nucleotides AO353_03815 and AO353_03820 are separated by 144 nucleotides AO353_03820 and AO353_03825 are separated by 9 nucleotides AO353_03825 and AO353_03830 are separated by 9 nucleotides AO353_03830 and AO353_03835 are separated by 335 nucleotides
AO353_03810: AO353_03810 - Alpha-ketoglutarate permease, MFS superfamily <small>(from data)</small>, at 815,502 to 816,821
_03810
AO353_03815: AO353_03815 - heme utilization protein, at 817,271 to 817,558
_03815
AO353_03820: AO353_03820 - Asp/Glu/hydantoin racemase, at 817,703 to 818,335
_03820
AO353_03825: AO353_03825 - hypothetical protein, at 818,345 to 818,608
_03825
AO353_03830: AO353_03830 - antibiotic biosynthesis monooxygenase, at 818,618 to 818,983
_03830
AO353_03835: AO353_03835 - AraC family transcriptional regulator, at 819,319 to 820,248
_03835
Position (kb) 817
818
819 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 816.729 kb on + strand at 816.729 kb on + strand at 816.730 kb on - strand at 816.730 kb on - strand at 816.730 kb on - strand at 816.735 kb on + strand at 816.735 kb on + strand at 816.735 kb on + strand at 816.735 kb on + strand at 816.735 kb on + strand at 816.736 kb on - strand at 816.736 kb on - strand at 816.736 kb on - strand at 816.736 kb on - strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.771 kb on + strand at 816.772 kb on - strand at 816.772 kb on - strand at 816.772 kb on - strand at 816.772 kb on - strand at 816.772 kb on - strand at 816.772 kb on - strand at 816.775 kb on + strand at 816.775 kb on + strand at 816.775 kb on + strand at 816.776 kb on - strand at 816.776 kb on - strand at 816.776 kb on - strand at 816.951 kb on + strand at 816.952 kb on - strand at 816.971 kb on - strand at 816.981 kb on + strand at 816.981 kb on + strand at 816.981 kb on + strand at 816.981 kb on + strand at 816.981 kb on + strand at 816.982 kb on - strand at 817.097 kb on + strand at 817.097 kb on + strand at 817.097 kb on + strand at 817.097 kb on + strand at 817.097 kb on + strand at 817.097 kb on + strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.098 kb on - strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.101 kb on + strand at 817.102 kb on - strand at 817.102 kb on - strand at 817.102 kb on - strand at 817.102 kb on - strand at 817.102 kb on - strand at 817.102 kb on - strand at 817.104 kb on - strand at 817.216 kb on + strand at 817.263 kb on + strand at 817.263 kb on + strand at 817.263 kb on + strand at 817.263 kb on + strand at 817.264 kb on - strand at 817.264 kb on - strand at 817.264 kb on - strand at 817.264 kb on - strand at 817.341 kb on - strand, within AO353_03815 at 817.404 kb on - strand, within AO353_03815 at 817.404 kb on - strand, within AO353_03815 at 817.556 kb on + strand at 817.556 kb on + strand at 817.557 kb on - strand at 817.557 kb on - strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.597 kb on + strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.598 kb on - strand at 817.664 kb on - strand at 817.664 kb on - strand at 817.681 kb on + strand at 817.681 kb on + strand at 817.681 kb on + strand at 817.681 kb on + strand at 817.681 kb on + strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.682 kb on - strand at 817.714 kb on + strand at 817.715 kb on - strand at 817.715 kb on - strand at 817.715 kb on - strand at 817.715 kb on - strand at 817.715 kb on - strand at 817.722 kb on - strand at 817.722 kb on - strand at 817.722 kb on - strand at 817.722 kb on - strand at 817.724 kb on + strand at 817.724 kb on + strand at 817.725 kb on - strand at 817.725 kb on - strand at 817.725 kb on - strand at 817.725 kb on - strand at 817.725 kb on - strand at 817.842 kb on - strand, within AO353_03820 at 817.842 kb on - strand, within AO353_03820 at 817.865 kb on + strand, within AO353_03820 at 817.865 kb on + strand, within AO353_03820 at 817.865 kb on + strand, within AO353_03820 at 817.866 kb on - strand, within AO353_03820 at 817.866 kb on - strand, within AO353_03820 at 817.866 kb on - strand, within AO353_03820 at 817.904 kb on - strand, within AO353_03820 at 818.274 kb on - strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.333 kb on + strand at 818.334 kb on - strand at 818.334 kb on - strand at 818.334 kb on - strand at 818.334 kb on - strand at 818.511 kb on + strand, within AO353_03825 at 818.512 kb on - strand, within AO353_03825 at 818.532 kb on - strand, within AO353_03825 at 818.566 kb on + strand, within AO353_03825 at 818.566 kb on + strand, within AO353_03825 at 818.566 kb on + strand, within AO353_03825 at 818.566 kb on + strand, within AO353_03825 at 818.567 kb on - strand, within AO353_03825 at 818.567 kb on - strand, within AO353_03825 at 818.611 kb on + strand at 818.611 kb on + strand at 818.612 kb on - strand at 818.612 kb on - strand at 818.636 kb on + strand at 818.636 kb on + strand at 818.636 kb on + strand at 818.637 kb on - strand at 818.735 kb on + strand, within AO353_03830 at 818.775 kb on + strand, within AO353_03830 at 818.776 kb on - strand, within AO353_03830 at 819.002 kb on + strand at 819.002 kb on + strand at 819.002 kb on + strand at 819.003 kb on - strand at 819.003 kb on - strand at 819.046 kb on + strand at 819.046 kb on + strand at 819.061 kb on - strand at 819.088 kb on + strand at 819.088 kb on + strand at 819.088 kb on + strand at 819.089 kb on - strand at 819.089 kb on - strand at 819.089 kb on - strand at 819.089 kb on - strand at 819.201 kb on + strand at 819.201 kb on + strand at 819.201 kb on + strand at 819.201 kb on + strand at 819.202 kb on - strand at 819.202 kb on - strand at 819.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0006 mg/ml remove 816,729 + -2.3 816,729 + +0.2 816,730 - -0.4 816,730 - +1.8 816,730 - -2.5 816,735 + +1.4 816,735 + -1.1 816,735 + -0.9 816,735 + -1.6 816,735 + -0.1 816,736 - -1.7 816,736 - +0.9 816,736 - -0.7 816,736 - -0.1 816,771 + +0.9 816,771 + +0.3 816,771 + -0.1 816,771 + -0.3 816,771 + -0.7 816,771 + +0.3 816,771 + +2.1 816,771 + +3.1 816,772 - -0.1 816,772 - -0.7 816,772 - -1.1 816,772 - +0.7 816,772 - -1.6 816,772 - +0.9 816,775 + -0.4 816,775 + -1.1 816,775 + -0.7 816,776 - -1.8 816,776 - +1.5 816,776 - +0.7 816,951 + +0.3 816,952 - -1.1 816,971 - +0.9 816,981 + -0.7 816,981 + -1.3 816,981 + -0.1 816,981 + +2.5 816,981 + -2.6 816,982 - +1.0 817,097 + -2.2 817,097 + +0.9 817,097 + +0.5 817,097 + +0.9 817,097 + +3.9 817,097 + +0.5 817,098 - +1.7 817,098 - +0.7 817,098 - +0.9 817,098 - +0.9 817,098 - +1.9 817,098 - +0.3 817,098 - -1.3 817,098 - -0.9 817,101 + +0.3 817,101 + -1.4 817,101 + -0.4 817,101 + -0.9 817,101 + -0.4 817,101 + +0.3 817,101 + -0.1 817,101 + -0.9 817,101 + +2.6 817,101 + -1.7 817,101 + -0.4 817,101 + +1.9 817,101 + +2.9 817,102 - -0.9 817,102 - -0.4 817,102 - -1.1 817,102 - -1.4 817,102 - +2.0 817,102 - -1.7 817,104 - -0.1 817,216 + +0.9 817,263 + +1.0 817,263 + -0.7 817,263 + -0.4 817,263 + -0.7 817,264 - +2.9 817,264 - +4.0 817,264 - +2.2 817,264 - +1.5 817,341 - AO353_03815 0.24 +1.5 817,404 - AO353_03815 0.46 -1.2 817,404 - AO353_03815 0.46 +1.9 817,556 + -1.7 817,556 + -1.4 817,557 - +1.9 817,557 - -0.1 817,597 + +0.1 817,597 + +1.7 817,597 + -0.4 817,597 + +0.9 817,597 + -1.3 817,597 + +1.9 817,597 + -0.9 817,597 + -1.7 817,597 + +0.3 817,597 + +1.6 817,598 - -0.2 817,598 - -0.4 817,598 - -0.0 817,598 - -2.0 817,598 - -1.3 817,598 - +2.3 817,598 - -0.7 817,664 - +1.9 817,664 - +0.9 817,681 + -1.9 817,681 + -2.4 817,681 + -0.1 817,681 + +0.3 817,681 + +1.9 817,682 - -0.7 817,682 - +2.7 817,682 - +3.1 817,682 - +0.7 817,682 - +0.3 817,682 - +0.4 817,682 - -0.1 817,714 + +2.1 817,715 - -0.4 817,715 - +0.1 817,715 - -0.5 817,715 - -1.1 817,715 - +0.2 817,722 - +1.9 817,722 - +1.3 817,722 - +0.0 817,722 - +0.3 817,724 + -0.1 817,724 + +1.4 817,725 - -2.1 817,725 - -0.4 817,725 - +0.9 817,725 - +1.9 817,725 - -1.1 817,842 - AO353_03820 0.22 -1.6 817,842 - AO353_03820 0.22 +1.6 817,865 + AO353_03820 0.26 +2.0 817,865 + AO353_03820 0.26 -0.7 817,865 + AO353_03820 0.26 -1.1 817,866 - AO353_03820 0.26 +5.3 817,866 - AO353_03820 0.26 +2.0 817,866 - AO353_03820 0.26 -0.9 817,904 - AO353_03820 0.32 +0.9 818,274 - -0.9 818,333 + +0.9 818,333 + -2.0 818,333 + -0.1 818,333 + -1.3 818,333 + +0.3 818,333 + -1.4 818,333 + +3.0 818,333 + +0.0 818,333 + -0.7 818,333 + +2.6 818,333 + -0.0 818,333 + +0.3 818,334 - +1.8 818,334 - -0.9 818,334 - +1.3 818,334 - -0.7 818,511 + AO353_03825 0.63 -1.6 818,512 - AO353_03825 0.63 -0.2 818,532 - AO353_03825 0.71 +0.3 818,566 + AO353_03825 0.84 -1.7 818,566 + AO353_03825 0.84 +0.3 818,566 + AO353_03825 0.84 +0.7 818,566 + AO353_03825 0.84 -0.7 818,567 - AO353_03825 0.84 +2.2 818,567 - AO353_03825 0.84 +2.0 818,611 + -0.4 818,611 + -0.7 818,612 - +2.0 818,612 - -1.3 818,636 + -0.3 818,636 + -0.7 818,636 + -1.3 818,637 - -0.4 818,735 + AO353_03830 0.32 +2.3 818,775 + AO353_03830 0.43 +0.9 818,776 - AO353_03830 0.43 -1.6 819,002 + -1.6 819,002 + +1.9 819,002 + +0.3 819,003 - +0.9 819,003 - -0.4 819,046 + -0.4 819,046 + -1.3 819,061 - -0.4 819,088 + -1.1 819,088 + -1.4 819,088 + -1.7 819,089 - -2.5 819,089 - +3.0 819,089 - +0.9 819,089 - -0.4 819,201 + +2.3 819,201 + +1.9 819,201 + -0.4 819,201 + -0.7 819,202 - -0.4 819,202 - +0.9 819,279 - -2.2
Or see this region's nucleotide sequence