Experiment: Doxycycline hyclate 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01035 and AO353_01040 are separated by 265 nucleotides AO353_01040 and AO353_01045 are separated by 35 nucleotides AO353_01045 and AO353_01050 are separated by 251 nucleotides
AO353_01035: AO353_01035 - hypothetical protein, at 227,927 to 228,136
_01035
AO353_01040: AO353_01040 - polyhydroxyalkanoate synthesis regulator, at 228,402 to 228,935
_01040
AO353_01045: AO353_01045 - phasin, at 228,971 to 229,534
_01045
AO353_01050: AO353_01050 - poly(3-hydroxyalkanoate) synthetase, at 229,786 to 232,104
_01050
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 228.026 kb on + strand, within AO353_01035 at 228.027 kb on - strand, within AO353_01035 at 228.027 kb on - strand, within AO353_01035 at 228.027 kb on - strand, within AO353_01035 at 228.083 kb on + strand, within AO353_01035 at 228.083 kb on + strand, within AO353_01035 at 228.083 kb on + strand, within AO353_01035 at 228.122 kb on - strand at 228.297 kb on + strand at 228.297 kb on + strand at 228.298 kb on - strand at 228.298 kb on - strand at 228.298 kb on - strand at 228.357 kb on + strand at 228.358 kb on - strand at 228.358 kb on - strand at 228.358 kb on - strand at 228.362 kb on + strand at 228.363 kb on - strand at 228.363 kb on - strand at 228.363 kb on - strand at 228.373 kb on + strand at 228.373 kb on + strand at 228.374 kb on - strand at 228.374 kb on - strand at 228.393 kb on + strand at 228.393 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.410 kb on + strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.411 kb on - strand at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand, within AO353_01040 at 228.460 kb on + strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.461 kb on - strand, within AO353_01040 at 228.462 kb on + strand, within AO353_01040 at 228.462 kb on + strand, within AO353_01040 at 228.462 kb on + strand, within AO353_01040 at 228.462 kb on + strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.463 kb on - strand, within AO353_01040 at 228.502 kb on + strand, within AO353_01040 at 228.502 kb on + strand, within AO353_01040 at 228.503 kb on - strand, within AO353_01040 at 228.503 kb on - strand, within AO353_01040 at 228.503 kb on - strand, within AO353_01040 at 228.503 kb on - strand, within AO353_01040 at 228.522 kb on - strand, within AO353_01040 at 228.522 kb on - strand, within AO353_01040 at 228.575 kb on + strand at 228.575 kb on + strand, within AO353_01040 at 228.575 kb on + strand, within AO353_01040 at 228.576 kb on - strand, within AO353_01040 at 228.660 kb on + strand, within AO353_01040 at 228.660 kb on + strand, within AO353_01040 at 228.660 kb on + strand, within AO353_01040 at 228.660 kb on + strand, within AO353_01040 at 228.661 kb on - strand, within AO353_01040 at 228.661 kb on - strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.690 kb on + strand, within AO353_01040 at 228.691 kb on - strand, within AO353_01040 at 228.691 kb on - strand, within AO353_01040 at 228.691 kb on - strand, within AO353_01040 at 228.691 kb on - strand, within AO353_01040 at 228.691 kb on - strand, within AO353_01040 at 228.698 kb on + strand, within AO353_01040 at 228.699 kb on - strand, within AO353_01040 at 228.813 kb on + strand, within AO353_01040 at 228.813 kb on + strand, within AO353_01040 at 228.813 kb on + strand, within AO353_01040 at 228.814 kb on - strand, within AO353_01040 at 228.814 kb on - strand, within AO353_01040 at 228.837 kb on + strand, within AO353_01040 at 228.837 kb on + strand, within AO353_01040 at 228.837 kb on + strand, within AO353_01040 at 228.838 kb on - strand, within AO353_01040 at 228.933 kb on + strand at 228.934 kb on - strand at 228.962 kb on + strand at 228.962 kb on + strand at 228.963 kb on - strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.972 kb on + strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 228.973 kb on - strand at 229.070 kb on + strand, within AO353_01045 at 229.304 kb on + strand, within AO353_01045 at 229.304 kb on + strand at 229.305 kb on - strand, within AO353_01045 at 229.305 kb on - strand, within AO353_01045 at 229.305 kb on - strand, within AO353_01045 at 229.305 kb on - strand, within AO353_01045 at 229.305 kb on - strand, within AO353_01045 at 229.305 kb on - strand at 229.305 kb on - strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.484 kb on + strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.485 kb on - strand at 229.566 kb on + strand at 229.566 kb on + strand at 229.607 kb on + strand at 229.608 kb on - strand at 229.613 kb on + strand at 229.613 kb on + strand at 229.614 kb on - strand at 229.614 kb on - strand at 229.614 kb on - strand at 229.616 kb on + strand at 229.687 kb on + strand at 229.696 kb on + strand at 229.696 kb on + strand at 229.696 kb on + strand at 229.697 kb on - strand at 229.697 kb on - strand at 229.697 kb on - strand at 229.697 kb on - strand at 229.697 kb on - strand at 229.697 kb on - strand at 229.768 kb on + strand at 229.769 kb on - strand at 229.769 kb on - strand at 229.769 kb on - strand at 229.800 kb on + strand at 229.800 kb on + strand at 229.800 kb on + strand at 229.801 kb on - strand at 229.801 kb on - strand at 229.801 kb on - strand at 229.803 kb on + strand at 229.803 kb on + strand at 229.803 kb on + strand at 229.803 kb on + strand at 229.804 kb on - strand at 229.804 kb on - strand at 229.816 kb on + strand at 229.816 kb on + strand at 229.816 kb on + strand at 229.817 kb on - strand at 229.817 kb on - strand at 229.818 kb on + strand at 229.818 kb on + strand at 229.879 kb on - strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.959 kb on + strand at 229.960 kb on - strand at 229.960 kb on - strand at 229.960 kb on - strand at 229.960 kb on - strand at 229.960 kb on - strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.981 kb on + strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 229.982 kb on - strand at 230.047 kb on - strand, within AO353_01050 at 230.047 kb on - strand, within AO353_01050 at 230.047 kb on - strand, within AO353_01050 at 230.140 kb on + strand, within AO353_01050 at 230.140 kb on + strand, within AO353_01050 at 230.140 kb on + strand, within AO353_01050 at 230.140 kb on + strand, within AO353_01050 at 230.141 kb on - strand, within AO353_01050 at 230.141 kb on - strand, within AO353_01050 at 230.141 kb on - strand, within AO353_01050 at 230.141 kb on - strand, within AO353_01050 at 230.141 kb on - strand, within AO353_01050 at 230.265 kb on - strand, within AO353_01050 at 230.267 kb on + strand, within AO353_01050 at 230.268 kb on - strand, within AO353_01050 at 230.268 kb on - strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.299 kb on + strand, within AO353_01050 at 230.300 kb on - strand, within AO353_01050 at 230.300 kb on - strand, within AO353_01050 at 230.300 kb on - strand, within AO353_01050 at 230.300 kb on - strand, within AO353_01050 at 230.300 kb on - strand, within AO353_01050 at 230.421 kb on + strand, within AO353_01050 at 230.422 kb on - strand, within AO353_01050 at 230.497 kb on - strand, within AO353_01050 at 230.518 kb on + strand, within AO353_01050
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0006 mg/ml remove 228,026 + AO353_01035 0.47 -0.9 228,027 - AO353_01035 0.48 +1.6 228,027 - AO353_01035 0.48 -1.2 228,027 - AO353_01035 0.48 -2.2 228,083 + AO353_01035 0.74 -1.7 228,083 + AO353_01035 0.74 +2.0 228,083 + AO353_01035 0.74 +0.6 228,122 - -1.4 228,297 + -0.5 228,297 + -1.0 228,298 - -0.9 228,298 - -0.9 228,298 - +2.1 228,357 + -0.4 228,358 - -0.9 228,358 - -0.4 228,358 - -1.2 228,362 + +0.2 228,363 - +0.1 228,363 - -0.7 228,363 - +1.3 228,373 + +1.6 228,373 + +0.8 228,374 - -0.4 228,374 - -2.9 228,393 + -1.7 228,393 + -1.8 228,410 + -0.7 228,410 + -1.4 228,410 + +0.6 228,410 + -1.5 228,410 + -0.8 228,410 + +1.8 228,410 + +2.3 228,411 - -0.9 228,411 - -1.9 228,411 - +2.5 228,411 - +1.8 228,411 - +0.6 228,411 - +0.6 228,411 - -1.8 228,460 + AO353_01040 0.11 +0.6 228,460 + AO353_01040 0.11 -1.8 228,460 + AO353_01040 0.11 -1.8 228,460 + AO353_01040 0.11 -0.4 228,460 + +1.6 228,460 + AO353_01040 0.11 +0.6 228,460 + AO353_01040 0.11 +2.3 228,460 + AO353_01040 0.11 -2.4 228,461 - AO353_01040 0.11 -0.4 228,461 - AO353_01040 0.11 +0.9 228,461 - AO353_01040 0.11 +0.1 228,461 - AO353_01040 0.11 -0.0 228,461 - AO353_01040 0.11 -1.7 228,461 - AO353_01040 0.11 -1.4 228,461 - AO353_01040 0.11 -0.9 228,461 - AO353_01040 0.11 +0.1 228,461 - AO353_01040 0.11 +0.9 228,462 + AO353_01040 0.11 -1.5 228,462 + AO353_01040 0.11 -1.2 228,462 + AO353_01040 0.11 +1.3 228,462 + AO353_01040 0.11 -0.5 228,463 - AO353_01040 0.11 -0.9 228,463 - AO353_01040 0.11 -1.2 228,463 - AO353_01040 0.11 +1.6 228,463 - AO353_01040 0.11 -2.3 228,463 - AO353_01040 0.11 -1.5 228,463 - AO353_01040 0.11 -2.3 228,463 - AO353_01040 0.11 -2.7 228,463 - AO353_01040 0.11 +0.3 228,463 - AO353_01040 0.11 -0.7 228,502 + AO353_01040 0.19 +0.6 228,502 + AO353_01040 0.19 +0.3 228,503 - AO353_01040 0.19 -1.2 228,503 - AO353_01040 0.19 +1.1 228,503 - AO353_01040 0.19 +0.1 228,503 - AO353_01040 0.19 -2.4 228,522 - AO353_01040 0.22 +0.2 228,522 - AO353_01040 0.22 +0.1 228,575 + +1.6 228,575 + AO353_01040 0.32 -0.4 228,575 + AO353_01040 0.32 +3.8 228,576 - AO353_01040 0.33 +1.8 228,660 + AO353_01040 0.48 -1.2 228,660 + AO353_01040 0.48 -2.1 228,660 + AO353_01040 0.48 +3.2 228,660 + AO353_01040 0.48 +3.2 228,661 - AO353_01040 0.49 +0.7 228,661 - AO353_01040 0.49 +2.1 228,690 + AO353_01040 0.54 +2.3 228,690 + AO353_01040 0.54 -2.4 228,690 + AO353_01040 0.54 -1.5 228,690 + AO353_01040 0.54 -0.8 228,690 + AO353_01040 0.54 +1.5 228,690 + AO353_01040 0.54 -0.4 228,691 - AO353_01040 0.54 -1.5 228,691 - AO353_01040 0.54 +0.1 228,691 - AO353_01040 0.54 -0.7 228,691 - AO353_01040 0.54 -0.7 228,691 - AO353_01040 0.54 +2.4 228,698 + AO353_01040 0.55 +0.4 228,699 - AO353_01040 0.56 -2.2 228,813 + AO353_01040 0.77 -2.8 228,813 + AO353_01040 0.77 +1.5 228,813 + AO353_01040 0.77 +0.6 228,814 - AO353_01040 0.77 -1.7 228,814 - AO353_01040 0.77 -0.0 228,837 + AO353_01040 0.81 -2.1 228,837 + AO353_01040 0.81 +0.6 228,837 + AO353_01040 0.81 -1.2 228,838 - AO353_01040 0.82 +3.2 228,933 + +0.6 228,934 - -0.7 228,962 + -1.9 228,962 + +1.6 228,963 - -0.7 228,972 + +0.1 228,972 + +1.6 228,972 + +0.1 228,972 + -0.7 228,972 + -1.3 228,972 + +0.1 228,972 + -0.9 228,972 + -1.2 228,972 + +2.1 228,972 + -2.3 228,972 + -1.9 228,973 - +3.2 228,973 - -2.2 228,973 - +2.0 228,973 - +2.5 228,973 - +0.6 228,973 - -0.2 228,973 - -0.7 228,973 - -1.7 228,973 - +2.6 229,070 + AO353_01045 0.18 +0.9 229,304 + AO353_01045 0.59 -0.4 229,304 + +0.1 229,305 - AO353_01045 0.59 -2.1 229,305 - AO353_01045 0.59 +0.6 229,305 - AO353_01045 0.59 -0.4 229,305 - AO353_01045 0.59 -2.2 229,305 - AO353_01045 0.59 -0.9 229,305 - +1.6 229,305 - +1.6 229,484 + -0.9 229,484 + -1.2 229,484 + +1.6 229,484 + -1.8 229,484 + +1.6 229,484 + +0.1 229,484 + +1.6 229,485 - -2.4 229,485 - -1.8 229,485 - +0.6 229,485 - -0.5 229,485 - -1.9 229,485 - -1.4 229,485 - -1.7 229,566 + +0.6 229,566 + +0.6 229,607 + +0.1 229,608 - +0.1 229,613 + -0.9 229,613 + -1.7 229,614 - -1.2 229,614 - -1.6 229,614 - +3.2 229,616 + +0.1 229,687 + +0.2 229,696 + -1.8 229,696 + -0.9 229,696 + +2.4 229,697 - +0.9 229,697 - +0.1 229,697 - -0.9 229,697 - -0.4 229,697 - +1.3 229,697 - -2.7 229,768 + -0.7 229,769 - +1.2 229,769 - -1.8 229,769 - -0.4 229,800 + -2.4 229,800 + -1.7 229,800 + -1.2 229,801 - -1.5 229,801 - -2.9 229,801 - +0.9 229,803 + -1.4 229,803 + -1.7 229,803 + +0.6 229,803 + -0.5 229,804 - +1.4 229,804 - +3.0 229,816 + +0.5 229,816 + +1.1 229,816 + -2.2 229,817 - +2.2 229,817 - +0.1 229,818 + -2.7 229,818 + -0.7 229,879 - +3.2 229,959 + +0.1 229,959 + -1.5 229,959 + -1.8 229,959 + +2.6 229,959 + -0.4 229,959 + +0.6 229,959 + +0.6 229,960 - -3.4 229,960 - -0.4 229,960 - -0.7 229,960 - +4.1 229,960 - -0.4 229,981 + +0.1 229,981 + +0.1 229,981 + +2.4 229,981 + -0.9 229,981 + -1.2 229,981 + -0.9 229,981 + -0.4 229,981 + -0.5 229,981 + -1.7 229,982 - +2.8 229,982 - +1.0 229,982 - +0.6 229,982 - +0.1 229,982 - +0.1 229,982 - -1.8 229,982 - +2.4 229,982 - +3.0 229,982 - -2.1 230,047 - AO353_01050 0.11 +0.1 230,047 - AO353_01050 0.11 -2.4 230,047 - AO353_01050 0.11 -0.9 230,140 + AO353_01050 0.15 +2.1 230,140 + AO353_01050 0.15 +1.0 230,140 + AO353_01050 0.15 -0.7 230,140 + AO353_01050 0.15 +2.4 230,141 - AO353_01050 0.15 +0.1 230,141 - AO353_01050 0.15 -0.4 230,141 - AO353_01050 0.15 -1.5 230,141 - AO353_01050 0.15 +1.6 230,141 - AO353_01050 0.15 +1.0 230,265 - AO353_01050 0.21 -0.4 230,267 + AO353_01050 0.21 +1.6 230,268 - AO353_01050 0.21 -1.9 230,268 - AO353_01050 0.21 +0.1 230,299 + AO353_01050 0.22 -1.2 230,299 + AO353_01050 0.22 -0.4 230,299 + AO353_01050 0.22 -0.9 230,299 + AO353_01050 0.22 -1.4 230,299 + AO353_01050 0.22 +0.8 230,299 + AO353_01050 0.22 +0.1 230,300 - AO353_01050 0.22 +2.3 230,300 - AO353_01050 0.22 -0.9 230,300 - AO353_01050 0.22 -0.9 230,300 - AO353_01050 0.22 -2.4 230,300 - AO353_01050 0.22 +0.7 230,421 + AO353_01050 0.27 +1.6 230,422 - AO353_01050 0.27 -0.9 230,497 - AO353_01050 0.31 -0.4 230,518 + AO353_01050 0.32 +0.1
Or see this region's nucleotide sequence