Experiment: Doxycycline hyclate 0.00078125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1951 and tRNA-Ser are separated by 90 nucleotides tRNA-Ser and Synpcc7942_1952 are separated by 33 nucleotides Synpcc7942_1952 and Synpcc7942_1953 are separated by 52 nucleotides Synpcc7942_1953 and pyrG are separated by 3 nucleotides
Synpcc7942_1951: Synpcc7942_1951 - hypothetical protein, at 2,024,828 to 2,025,190
_1951
Synpcc7942_R0002: tRNA-Ser - tRNA-Ser, at 2,025,281 to 2,025,365
tRNA-Ser
Synpcc7942_1952: Synpcc7942_1952 - hypothetical protein, at 2,025,399 to 2,025,740
_1952
Synpcc7942_1953: Synpcc7942_1953 - 6-pyruvoyl tetrahydrobiopterin synthase, at 2,025,793 to 2,026,179
_1953
Synpcc7942_1954: pyrG - CTP synthetase, at 2,026,183 to 2,027,877
pyrG
Position (kb)
2025
2026
2027 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2024.793 kb on - strand at 2024.795 kb on + strand at 2024.813 kb on + strand at 2024.813 kb on + strand at 2024.813 kb on + strand at 2024.821 kb on - strand at 2024.909 kb on + strand, within Synpcc7942_1951 at 2024.917 kb on - strand, within Synpcc7942_1951 at 2024.917 kb on - strand, within Synpcc7942_1951 at 2024.917 kb on - strand, within Synpcc7942_1951 at 2024.917 kb on - strand, within Synpcc7942_1951 at 2024.949 kb on - strand, within Synpcc7942_1951 at 2024.956 kb on + strand, within Synpcc7942_1951 at 2024.965 kb on - strand, within Synpcc7942_1951 at 2024.975 kb on - strand, within Synpcc7942_1951 at 2025.018 kb on + strand, within Synpcc7942_1951 at 2025.065 kb on + strand, within Synpcc7942_1951 at 2025.091 kb on + strand, within Synpcc7942_1951 at 2025.091 kb on + strand, within Synpcc7942_1951 at 2025.099 kb on - strand, within Synpcc7942_1951 at 2025.108 kb on + strand, within Synpcc7942_1951 at 2025.108 kb on + strand, within Synpcc7942_1951 at 2025.125 kb on + strand, within Synpcc7942_1951 at 2025.131 kb on + strand, within Synpcc7942_1951 at 2025.133 kb on - strand, within Synpcc7942_1951 at 2025.142 kb on + strand, within Synpcc7942_1951 at 2025.155 kb on - strand at 2025.156 kb on + strand at 2025.184 kb on + strand at 2025.193 kb on - strand at 2025.227 kb on - strand at 2025.574 kb on + strand, within Synpcc7942_1952 at 2025.575 kb on - strand, within Synpcc7942_1952 at 2025.643 kb on - strand, within Synpcc7942_1952 at 2025.693 kb on - strand, within Synpcc7942_1952 at 2025.694 kb on + strand, within Synpcc7942_1952 at 2025.747 kb on + strand at 2025.771 kb on - strand at 2025.798 kb on - strand at 2025.799 kb on - strand at 2025.839 kb on + strand, within Synpcc7942_1953 at 2025.839 kb on + strand, within Synpcc7942_1953 at 2025.847 kb on - strand, within Synpcc7942_1953 at 2025.892 kb on - strand, within Synpcc7942_1953 at 2025.892 kb on - strand, within Synpcc7942_1953 at 2025.914 kb on + strand, within Synpcc7942_1953 at 2025.922 kb on - strand, within Synpcc7942_1953 at 2025.928 kb on - strand, within Synpcc7942_1953 at 2025.948 kb on - strand, within Synpcc7942_1953 at 2025.948 kb on - strand, within Synpcc7942_1953 at 2025.960 kb on - strand, within Synpcc7942_1953 at 2025.960 kb on - strand, within Synpcc7942_1953 at 2025.960 kb on - strand, within Synpcc7942_1953 at 2025.989 kb on - strand, within Synpcc7942_1953 at 2025.999 kb on - strand, within Synpcc7942_1953 at 2026.033 kb on + strand, within Synpcc7942_1953 at 2026.035 kb on + strand, within Synpcc7942_1953 at 2026.041 kb on - strand, within Synpcc7942_1953 at 2026.045 kb on + strand, within Synpcc7942_1953 at 2026.050 kb on + strand, within Synpcc7942_1953 at 2026.077 kb on + strand, within Synpcc7942_1953 at 2026.079 kb on + strand, within Synpcc7942_1953 at 2026.085 kb on + strand, within Synpcc7942_1953 at 2026.085 kb on - strand, within Synpcc7942_1953 at 2026.087 kb on - strand, within Synpcc7942_1953 at 2026.087 kb on - strand, within Synpcc7942_1953 at 2026.087 kb on - strand, within Synpcc7942_1953 at 2026.120 kb on + strand, within Synpcc7942_1953 at 2026.120 kb on - strand, within Synpcc7942_1953 at 2026.123 kb on - strand, within Synpcc7942_1953 at 2026.164 kb on + strand at 2026.208 kb on - strand at 2026.234 kb on + strand at 2026.239 kb on + strand at 2026.247 kb on - strand at 2026.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.00078125 mg/ml remove 2,024,793 - +1.1 2,024,795 + +0.3 2,024,813 + -0.3 2,024,813 + +0.5 2,024,813 + +0.7 2,024,821 - +1.0 2,024,909 + Synpcc7942_1951 0.22 -2.1 2,024,917 - Synpcc7942_1951 0.25 +0.4 2,024,917 - Synpcc7942_1951 0.25 +2.2 2,024,917 - Synpcc7942_1951 0.25 +2.5 2,024,917 - Synpcc7942_1951 0.25 -1.1 2,024,949 - Synpcc7942_1951 0.33 -0.3 2,024,956 + Synpcc7942_1951 0.35 -1.1 2,024,965 - Synpcc7942_1951 0.38 +0.3 2,024,975 - Synpcc7942_1951 0.40 +0.3 2,025,018 + Synpcc7942_1951 0.52 -1.8 2,025,065 + Synpcc7942_1951 0.65 -0.5 2,025,091 + Synpcc7942_1951 0.72 -0.1 2,025,091 + Synpcc7942_1951 0.72 -1.1 2,025,099 - Synpcc7942_1951 0.75 -3.1 2,025,108 + Synpcc7942_1951 0.77 +0.5 2,025,108 + Synpcc7942_1951 0.77 +0.1 2,025,125 + Synpcc7942_1951 0.82 -1.1 2,025,131 + Synpcc7942_1951 0.83 +1.7 2,025,133 - Synpcc7942_1951 0.84 -1.7 2,025,142 + Synpcc7942_1951 0.87 +1.2 2,025,155 - -0.9 2,025,156 + +0.6 2,025,184 + -1.1 2,025,193 - +0.1 2,025,227 - -0.6 2,025,574 + Synpcc7942_1952 0.51 -0.1 2,025,575 - Synpcc7942_1952 0.51 -1.7 2,025,643 - Synpcc7942_1952 0.71 -1.7 2,025,693 - Synpcc7942_1952 0.86 -0.2 2,025,694 + Synpcc7942_1952 0.86 -1.4 2,025,747 + +0.7 2,025,771 - -1.1 2,025,798 - +1.9 2,025,799 - -0.4 2,025,839 + Synpcc7942_1953 0.12 -0.3 2,025,839 + Synpcc7942_1953 0.12 -0.1 2,025,847 - Synpcc7942_1953 0.14 -5.7 2,025,892 - Synpcc7942_1953 0.26 -0.9 2,025,892 - Synpcc7942_1953 0.26 -2.1 2,025,914 + Synpcc7942_1953 0.31 -2.8 2,025,922 - Synpcc7942_1953 0.33 -1.0 2,025,928 - Synpcc7942_1953 0.35 -3.0 2,025,948 - Synpcc7942_1953 0.40 -2.0 2,025,948 - Synpcc7942_1953 0.40 -2.6 2,025,960 - Synpcc7942_1953 0.43 -0.8 2,025,960 - Synpcc7942_1953 0.43 -0.7 2,025,960 - Synpcc7942_1953 0.43 -1.1 2,025,989 - Synpcc7942_1953 0.51 -1.9 2,025,999 - Synpcc7942_1953 0.53 -2.9 2,026,033 + Synpcc7942_1953 0.62 -0.4 2,026,035 + Synpcc7942_1953 0.63 -1.1 2,026,041 - Synpcc7942_1953 0.64 -1.9 2,026,045 + Synpcc7942_1953 0.65 -1.7 2,026,050 + Synpcc7942_1953 0.66 -0.7 2,026,077 + Synpcc7942_1953 0.73 -2.1 2,026,079 + Synpcc7942_1953 0.74 -0.5 2,026,085 + Synpcc7942_1953 0.75 -3.1 2,026,085 - Synpcc7942_1953 0.75 -0.7 2,026,087 - Synpcc7942_1953 0.76 -1.7 2,026,087 - Synpcc7942_1953 0.76 -1.0 2,026,087 - Synpcc7942_1953 0.76 -1.8 2,026,120 + Synpcc7942_1953 0.84 -2.0 2,026,120 - Synpcc7942_1953 0.84 -1.8 2,026,123 - Synpcc7942_1953 0.85 -1.4 2,026,164 + -1.7 2,026,208 - +0.9 2,026,234 + -1.1 2,026,239 + -1.5 2,026,247 - -3.5 2,026,252 - -1.7
Or see this region's nucleotide sequence