Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_633 and Pf1N1B4_634 overlap by 1 nucleotides Pf1N1B4_634 and Pf1N1B4_635 are separated by 14 nucleotides Pf1N1B4_635 and Pf1N1B4_636 overlap by 11 nucleotides Pf1N1B4_636 and Pf1N1B4_637 are separated by 127 nucleotides
Pf1N1B4_633 - TolA protein, at 730,082 to 731,161
_633
Pf1N1B4_634 - Tol biopolymer transport system, TolR protein, at 731,161 to 731,622
_634
Pf1N1B4_635 - MotA/TolQ/ExbB proton channel family protein, at 731,637 to 732,332
_635
Pf1N1B4_636 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 732,322 to 732,789
_636
Pf1N1B4_637 - Holliday junction DNA helicase RuvB, at 732,917 to 733,978
_637
Position (kb)
731
732
733 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 731.639 kb on - strand at 731.639 kb on - strand at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.475 kb on - strand, within Pf1N1B4_636 at 732.533 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.696 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.795 kb on - strand at 732.795 kb on - strand at 732.802 kb on - strand at 732.802 kb on - strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.915 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.947 kb on + strand at 733.044 kb on + strand, within Pf1N1B4_637 at 733.062 kb on + strand, within Pf1N1B4_637
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 731,639 - -0.3 731,639 - +0.8 732,392 - Pf1N1B4_636 0.15 +1.4 732,392 - Pf1N1B4_636 0.15 -1.6 732,392 - Pf1N1B4_636 0.15 -0.9 732,392 - Pf1N1B4_636 0.15 -0.3 732,475 - Pf1N1B4_636 0.33 +1.5 732,533 - Pf1N1B4_636 0.45 -1.6 732,685 - Pf1N1B4_636 0.78 -0.0 732,685 - Pf1N1B4_636 0.78 -1.0 732,696 - Pf1N1B4_636 0.80 +0.5 732,705 - Pf1N1B4_636 0.82 -1.0 732,705 - Pf1N1B4_636 0.82 -1.0 732,708 - Pf1N1B4_636 0.82 -0.7 732,708 - Pf1N1B4_636 0.82 -0.0 732,708 - Pf1N1B4_636 0.82 -0.3 732,708 - Pf1N1B4_636 0.82 -1.0 732,790 - -1.6 732,790 - -1.4 732,790 - -2.0 732,790 - -0.5 732,790 - +0.5 732,795 - +0.0 732,795 - +1.0 732,802 - -0.5 732,802 - +0.3 732,867 + -2.4 732,867 + -1.0 732,867 + -2.8 732,896 + +0.3 732,896 + -2.0 732,896 + -2.2 732,896 + -0.7 732,915 + -2.4 732,918 + -1.0 732,918 + +0.5 732,918 + -1.6 732,918 + +1.0 732,918 + -0.2 732,947 + -1.6 733,044 + Pf1N1B4_637 0.12 -1.0 733,062 + Pf1N1B4_637 0.14 -0.0
Or see this region's nucleotide sequence