Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotides Pf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotides Pf1N1B4_4322 and Pf1N1B4_4323 are separated by 15 nucleotides Pf1N1B4_4323 and Pf1N1B4_4324 overlap by 31 nucleotides
Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928
_4320
Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719
_4321
Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567
_4322
Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744
_4323
Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580
_4324
Position (kb)
4783
4784
4785 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4783.150 kb on - strand at 4783.235 kb on + strand, within Pf1N1B4_4321 at 4783.236 kb on - strand, within Pf1N1B4_4321 at 4783.762 kb on + strand at 4783.763 kb on - strand at 4783.862 kb on + strand, within Pf1N1B4_4322 at 4784.087 kb on - strand at 4784.087 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand at 4784.122 kb on + strand, within Pf1N1B4_4322 at 4784.419 kb on + strand, within Pf1N1B4_4322 at 4784.582 kb on + strand at 4784.582 kb on + strand at 4784.715 kb on + strand at 4784.715 kb on + strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.879 kb on + strand at 4784.972 kb on + strand, within Pf1N1B4_4324 at 4785.160 kb on + strand, within Pf1N1B4_4324 at 4785.206 kb on + strand at 4785.206 kb on + strand at 4785.441 kb on + strand, within Pf1N1B4_4324
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 4,783,150 - -0.1 4,783,235 + Pf1N1B4_4321 0.39 +0.1 4,783,236 - Pf1N1B4_4321 0.39 -2.1 4,783,762 + -1.1 4,783,763 - -2.1 4,783,862 + Pf1N1B4_4322 0.17 -0.2 4,784,087 - -0.7 4,784,087 - Pf1N1B4_4322 0.44 -1.1 4,784,118 - Pf1N1B4_4322 0.47 +0.6 4,784,118 - -0.1 4,784,122 + Pf1N1B4_4322 0.48 +1.9 4,784,419 + Pf1N1B4_4322 0.83 -0.7 4,784,582 + +0.7 4,784,582 + +0.3 4,784,715 + +0.9 4,784,715 + +1.9 4,784,716 - -1.1 4,784,716 - +0.9 4,784,716 - +0.2 4,784,879 + -1.1 4,784,972 + Pf1N1B4_4324 0.30 +0.4 4,785,160 + Pf1N1B4_4324 0.51 +1.9 4,785,206 + -0.1 4,785,206 + -0.1 4,785,441 + Pf1N1B4_4324 0.84 -0.8
Or see this region's nucleotide sequence