Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4322

Experiment: octanoate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotidesPf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotidesPf1N1B4_4322 and Pf1N1B4_4323 are separated by 15 nucleotidesPf1N1B4_4323 and Pf1N1B4_4324 overlap by 31 nucleotides Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928 _4320 Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719 _4321 Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567 _4322 Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744 _4323 Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580 _4324 Position (kb) 4783 4784 4785Strain fitness (log2 ratio) -2 -1 0 1 2at 4783.150 kb on - strandat 4783.235 kb on + strand, within Pf1N1B4_4321at 4783.236 kb on - strand, within Pf1N1B4_4321at 4783.762 kb on + strandat 4783.763 kb on - strandat 4783.862 kb on + strand, within Pf1N1B4_4322at 4784.087 kb on - strandat 4784.087 kb on - strand, within Pf1N1B4_4322at 4784.118 kb on - strand, within Pf1N1B4_4322at 4784.118 kb on - strandat 4784.122 kb on + strand, within Pf1N1B4_4322at 4784.419 kb on + strand, within Pf1N1B4_4322at 4784.582 kb on + strandat 4784.582 kb on + strandat 4784.715 kb on + strandat 4784.715 kb on + strandat 4784.716 kb on - strandat 4784.716 kb on - strandat 4784.716 kb on - strandat 4784.879 kb on + strandat 4784.972 kb on + strand, within Pf1N1B4_4324at 4785.160 kb on + strand, within Pf1N1B4_4324at 4785.206 kb on + strandat 4785.206 kb on + strandat 4785.441 kb on + strand, within Pf1N1B4_4324

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Per-strain Table

Position Strand Gene LocusTag Fraction octanoate (C)
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4,783,150 - -0.1
4,783,235 + Pf1N1B4_4321 0.39 +0.1
4,783,236 - Pf1N1B4_4321 0.39 -2.1
4,783,762 + -1.1
4,783,763 - -2.1
4,783,862 + Pf1N1B4_4322 0.17 -0.2
4,784,087 - -0.7
4,784,087 - Pf1N1B4_4322 0.44 -1.1
4,784,118 - Pf1N1B4_4322 0.47 +0.6
4,784,118 - -0.1
4,784,122 + Pf1N1B4_4322 0.48 +1.9
4,784,419 + Pf1N1B4_4322 0.83 -0.7
4,784,582 + +0.7
4,784,582 + +0.3
4,784,715 + +0.9
4,784,715 + +1.9
4,784,716 - -1.1
4,784,716 - +0.9
4,784,716 - +0.2
4,784,879 + -1.1
4,784,972 + Pf1N1B4_4324 0.30 +0.4
4,785,160 + Pf1N1B4_4324 0.51 +1.9
4,785,206 + -0.1
4,785,206 + -0.1
4,785,441 + Pf1N1B4_4324 0.84 -0.8

Or see this region's nucleotide sequence