Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4221 and Pf1N1B4_4222 are separated by 19 nucleotides Pf1N1B4_4222 and Pf1N1B4_4223 are separated by 6 nucleotides Pf1N1B4_4223 and Pf1N1B4_4224 are separated by 306 nucleotides Pf1N1B4_4224 and Pf1N1B4_4225 are separated by 149 nucleotides
Pf1N1B4_4221 - hypothetical protein, at 4,678,383 to 4,678,499
_4221
Pf1N1B4_4222 - putative tyrosinase, at 4,678,519 to 4,679,379
_4222
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Pf1N1B4_4224 - putative tautomerase, at 4,680,586 to 4,680,795
_4224
Pf1N1B4_4225 - transcriptional regulator, AraC family, at 4,680,945 to 4,681,907
_4225
Position (kb)
4679
4680
4681 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.498 kb on - strand at 4678.531 kb on + strand at 4678.604 kb on - strand at 4678.604 kb on - strand at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4679.011 kb on + strand, within Pf1N1B4_4222 at 4679.012 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.098 kb on - strand, within Pf1N1B4_4222 at 4679.230 kb on + strand, within Pf1N1B4_4222 at 4679.231 kb on - strand at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.335 kb on + strand at 4679.336 kb on - strand at 4679.345 kb on - strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.353 kb on + strand at 4679.377 kb on + strand at 4679.377 kb on + strand at 4679.501 kb on + strand, within Pf1N1B4_4223 at 4679.502 kb on - strand at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.807 kb on + strand, within Pf1N1B4_4223 at 4679.884 kb on - strand at 4679.933 kb on + strand at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand at 4680.380 kb on - strand at 4680.403 kb on - strand at 4680.433 kb on + strand at 4680.433 kb on + strand at 4680.437 kb on + strand at 4680.437 kb on + strand at 4680.438 kb on - strand at 4680.542 kb on + strand at 4680.793 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.947 kb on - strand at 4680.982 kb on + strand at 4680.982 kb on + strand at 4681.083 kb on + strand at 4681.083 kb on + strand, within Pf1N1B4_4225
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 4,678,497 + +0.5 4,678,497 + -0.7 4,678,497 + -1.4 4,678,497 + +1.5 4,678,498 - -1.1 4,678,531 + -2.0 4,678,604 - +1.7 4,678,604 - -0.8 4,678,846 + Pf1N1B4_4222 0.38 -0.7 4,678,846 + Pf1N1B4_4222 0.38 -0.7 4,679,011 + Pf1N1B4_4222 0.57 -0.1 4,679,012 - Pf1N1B4_4222 0.57 -2.9 4,679,027 - Pf1N1B4_4222 0.59 -0.5 4,679,027 - Pf1N1B4_4222 0.59 -0.3 4,679,027 - Pf1N1B4_4222 0.59 -0.1 4,679,098 - Pf1N1B4_4222 0.67 -1.1 4,679,230 + Pf1N1B4_4222 0.83 -0.1 4,679,231 - -1.7 4,679,234 + Pf1N1B4_4222 0.83 +1.9 4,679,234 + Pf1N1B4_4222 0.83 +1.2 4,679,235 - Pf1N1B4_4222 0.83 -0.1 4,679,235 - Pf1N1B4_4222 0.83 +0.5 4,679,290 + Pf1N1B4_4222 0.90 -1.1 4,679,290 + Pf1N1B4_4222 0.90 +1.2 4,679,291 - Pf1N1B4_4222 0.90 -2.4 4,679,291 - Pf1N1B4_4222 0.90 +0.5 4,679,291 - Pf1N1B4_4222 0.90 -2.4 4,679,335 + -1.1 4,679,336 - -0.1 4,679,345 - -3.2 4,679,347 + -0.3 4,679,347 + +0.5 4,679,347 + +1.9 4,679,347 + -2.7 4,679,348 - -0.1 4,679,348 - +0.9 4,679,348 - -1.9 4,679,353 + -2.1 4,679,377 + -2.1 4,679,377 + +2.2 4,679,501 + Pf1N1B4_4223 0.13 +2.5 4,679,502 - -0.5 4,679,734 + Pf1N1B4_4223 0.39 -0.1 4,679,734 + Pf1N1B4_4223 0.39 +0.5 4,679,807 + Pf1N1B4_4223 0.47 -0.5 4,679,884 - -1.7 4,679,933 + -0.1 4,679,934 - Pf1N1B4_4223 0.61 +1.9 4,679,934 - Pf1N1B4_4223 0.61 -0.1 4,680,137 - Pf1N1B4_4223 0.84 -0.1 4,680,244 - +1.1 4,680,303 + +1.2 4,680,380 - -0.3 4,680,403 - +0.2 4,680,433 + -1.1 4,680,433 + -0.1 4,680,437 + +1.5 4,680,437 + -1.1 4,680,438 - -1.7 4,680,542 + +0.5 4,680,793 + -1.1 4,680,946 + -0.9 4,680,946 + +0.3 4,680,946 + -1.7 4,680,947 - -0.1 4,680,982 + -2.9 4,680,982 + +1.5 4,681,083 + -0.1 4,681,083 + Pf1N1B4_4225 0.14 -1.7
Or see this region's nucleotide sequence