Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_346 and Pf1N1B4_347 are separated by 120 nucleotides Pf1N1B4_347 and Pf1N1B4_348 are separated by 65 nucleotides Pf1N1B4_348 and Pf1N1B4_349 are separated by 28 nucleotides
Pf1N1B4_346 - SSU ribosomal protein S1p, at 409,382 to 411,073
_346
Pf1N1B4_347 - Cytidylate kinase (EC 2.7.4.25), at 411,194 to 411,814
_347
Pf1N1B4_348 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 411,880 to 414,087
_348
Pf1N1B4_349 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 414,116 to 415,228
_349
Position (kb)
411
412
413
414
415 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 411.167 kb on - strand at 411.167 kb on - strand at 411.167 kb on - strand at 412.650 kb on - strand, within Pf1N1B4_348 at 412.650 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.282 kb on - strand, within Pf1N1B4_348 at 413.344 kb on - strand, within Pf1N1B4_348 at 413.344 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.951 kb on - strand at 413.951 kb on - strand at 414.087 kb on + strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.211 kb on - strand at 414.396 kb on + strand, within Pf1N1B4_349 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.585 kb on + strand, within Pf1N1B4_349 at 414.586 kb on - strand, within Pf1N1B4_349 at 414.622 kb on - strand, within Pf1N1B4_349 at 414.630 kb on + strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.852 kb on + strand, within Pf1N1B4_349 at 414.904 kb on - strand, within Pf1N1B4_349
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 411,167 - +0.3 411,167 - -0.2 411,167 - -1.6 412,650 - Pf1N1B4_348 0.35 -3.4 412,650 - Pf1N1B4_348 0.35 -2.0 413,214 - Pf1N1B4_348 0.60 -1.6 413,214 - Pf1N1B4_348 0.60 -0.6 413,214 - Pf1N1B4_348 0.60 -0.7 413,282 - Pf1N1B4_348 0.63 +0.4 413,344 - Pf1N1B4_348 0.66 -2.0 413,344 - Pf1N1B4_348 0.66 -2.8 413,662 - Pf1N1B4_348 0.81 -1.0 413,662 - Pf1N1B4_348 0.81 -1.0 413,662 - Pf1N1B4_348 0.81 -1.9 413,662 - Pf1N1B4_348 0.81 +0.0 413,662 - Pf1N1B4_348 0.81 -1.6 413,951 - -1.0 413,951 - -0.6 414,087 + -0.6 414,088 - -1.6 414,088 - +0.6 414,088 - +0.0 414,088 - -0.3 414,088 - +1.6 414,088 - +0.5 414,088 - -1.0 414,088 - -1.3 414,088 - -1.6 414,088 - +1.6 414,088 - +0.0 414,088 - +1.0 414,088 - +0.0 414,088 - -1.6 414,211 - +1.3 414,396 + Pf1N1B4_349 0.25 -1.6 414,396 + Pf1N1B4_349 0.25 -2.6 414,397 - Pf1N1B4_349 0.25 -1.9 414,397 - Pf1N1B4_349 0.25 -0.2 414,535 - Pf1N1B4_349 0.38 -3.0 414,535 - Pf1N1B4_349 0.38 -2.0 414,585 + Pf1N1B4_349 0.42 -1.6 414,586 - Pf1N1B4_349 0.42 -1.3 414,622 - Pf1N1B4_349 0.45 +1.6 414,630 + Pf1N1B4_349 0.46 -1.0 414,631 - Pf1N1B4_349 0.46 -1.0 414,631 - Pf1N1B4_349 0.46 +1.2 414,631 - Pf1N1B4_349 0.46 -1.0 414,631 - Pf1N1B4_349 0.46 +1.0 414,852 + Pf1N1B4_349 0.66 +1.0 414,904 - Pf1N1B4_349 0.71 -0.7
Or see this region's nucleotide sequence