Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1447 and Pf1N1B4_1448 are separated by 464 nucleotides Pf1N1B4_1448 and Pf1N1B4_1449 are separated by 160 nucleotides
Pf1N1B4_1447 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 1,658,551 to 1,660,440
_1447
Pf1N1B4_1448 - Type I secretion outer membrane protein, TolC precursor, at 1,660,905 to 1,662,344
_1448
Pf1N1B4_1449 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 1,662,505 to 1,663,785
_1449
Position (kb)
1660
1661
1662
1663 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.971 kb on + strand, within Pf1N1B4_1447 at 1659.972 kb on - strand, within Pf1N1B4_1447 at 1659.993 kb on + strand, within Pf1N1B4_1447 at 1659.994 kb on - strand, within Pf1N1B4_1447 at 1660.008 kb on - strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.223 kb on + strand, within Pf1N1B4_1447 at 1660.224 kb on - strand, within Pf1N1B4_1447 at 1660.241 kb on + strand at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.241 kb on + strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.242 kb on - strand, within Pf1N1B4_1447 at 1660.346 kb on + strand at 1660.346 kb on + strand at 1660.347 kb on - strand at 1660.495 kb on + strand at 1660.669 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.687 kb on + strand at 1660.688 kb on - strand at 1660.688 kb on - strand at 1660.721 kb on - strand at 1660.721 kb on - strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.809 kb on + strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1660.810 kb on - strand at 1661.196 kb on + strand, within Pf1N1B4_1448 at 1661.197 kb on - strand, within Pf1N1B4_1448 at 1661.565 kb on + strand, within Pf1N1B4_1448 at 1661.565 kb on + strand, within Pf1N1B4_1448 at 1661.566 kb on - strand, within Pf1N1B4_1448 at 1661.566 kb on - strand, within Pf1N1B4_1448 at 1661.682 kb on + strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.683 kb on - strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.760 kb on + strand, within Pf1N1B4_1448 at 1661.761 kb on - strand, within Pf1N1B4_1448 at 1661.761 kb on - strand, within Pf1N1B4_1448 at 1661.875 kb on - strand, within Pf1N1B4_1448 at 1661.875 kb on - strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand, within Pf1N1B4_1448 at 1662.135 kb on + strand at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.136 kb on - strand at 1662.136 kb on - strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.153 kb on + strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.154 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.175 kb on - strand, within Pf1N1B4_1448 at 1662.264 kb on + strand at 1662.264 kb on + strand at 1662.342 kb on + strand at 1662.343 kb on - strand at 1662.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 1,659,971 + Pf1N1B4_1447 0.75 -1.0 1,659,971 + Pf1N1B4_1447 0.75 -2.8 1,659,971 + Pf1N1B4_1447 0.75 +0.6 1,659,972 - Pf1N1B4_1447 0.75 -0.0 1,659,993 + Pf1N1B4_1447 0.76 -0.2 1,659,994 - Pf1N1B4_1447 0.76 -1.6 1,660,008 - Pf1N1B4_1447 0.77 -0.4 1,660,223 + Pf1N1B4_1447 0.88 +0.7 1,660,223 + Pf1N1B4_1447 0.88 -0.0 1,660,223 + Pf1N1B4_1447 0.88 -0.6 1,660,224 - Pf1N1B4_1447 0.89 -0.0 1,660,241 + -0.0 1,660,241 + Pf1N1B4_1447 0.89 -1.4 1,660,241 + Pf1N1B4_1447 0.89 -1.8 1,660,241 + Pf1N1B4_1447 0.89 -0.6 1,660,241 + Pf1N1B4_1447 0.89 +0.4 1,660,242 - Pf1N1B4_1447 0.89 -1.2 1,660,242 - Pf1N1B4_1447 0.89 +1.6 1,660,242 - Pf1N1B4_1447 0.89 +0.7 1,660,242 - -0.0 1,660,242 - Pf1N1B4_1447 0.89 -1.0 1,660,242 - Pf1N1B4_1447 0.89 +0.5 1,660,346 + -1.0 1,660,346 + -0.0 1,660,347 - +0.1 1,660,495 + -1.0 1,660,669 + -0.8 1,660,687 + +0.3 1,660,687 + -0.0 1,660,687 + -0.0 1,660,687 + +1.8 1,660,688 - -1.6 1,660,688 - -0.3 1,660,721 - -1.0 1,660,721 - +1.0 1,660,809 + +0.7 1,660,809 + +1.8 1,660,809 + +0.6 1,660,809 + -0.0 1,660,810 - -0.0 1,660,810 - +1.6 1,660,810 - -1.6 1,660,810 - -2.3 1,660,810 - +1.8 1,660,810 - -1.0 1,661,196 + Pf1N1B4_1448 0.20 -3.2 1,661,197 - Pf1N1B4_1448 0.20 -1.2 1,661,565 + Pf1N1B4_1448 0.46 -2.6 1,661,565 + Pf1N1B4_1448 0.46 -0.6 1,661,566 - Pf1N1B4_1448 0.46 +0.5 1,661,566 - Pf1N1B4_1448 0.46 +0.3 1,661,682 + Pf1N1B4_1448 0.54 -1.0 1,661,683 - Pf1N1B4_1448 0.54 +0.7 1,661,683 - Pf1N1B4_1448 0.54 -1.3 1,661,683 - Pf1N1B4_1448 0.54 +1.0 1,661,683 - Pf1N1B4_1448 0.54 -1.6 1,661,683 - Pf1N1B4_1448 0.54 -0.8 1,661,683 - Pf1N1B4_1448 0.54 -1.0 1,661,683 - Pf1N1B4_1448 0.54 -2.3 1,661,683 - Pf1N1B4_1448 0.54 -0.6 1,661,760 + Pf1N1B4_1448 0.59 +1.8 1,661,760 + Pf1N1B4_1448 0.59 -0.0 1,661,760 + Pf1N1B4_1448 0.59 +2.0 1,661,761 - Pf1N1B4_1448 0.59 -2.6 1,661,761 - Pf1N1B4_1448 0.59 -0.7 1,661,875 - Pf1N1B4_1448 0.67 -1.0 1,661,875 - Pf1N1B4_1448 0.67 -0.8 1,662,135 + Pf1N1B4_1448 0.85 -0.0 1,662,135 + Pf1N1B4_1448 0.85 -0.0 1,662,135 + Pf1N1B4_1448 0.85 -0.4 1,662,135 + Pf1N1B4_1448 0.85 -0.0 1,662,135 + Pf1N1B4_1448 0.85 +0.6 1,662,135 + -0.0 1,662,136 - Pf1N1B4_1448 0.85 -2.8 1,662,136 - Pf1N1B4_1448 0.85 +0.5 1,662,136 - Pf1N1B4_1448 0.85 -3.0 1,662,136 - -0.0 1,662,136 - Pf1N1B4_1448 0.85 -0.2 1,662,153 + Pf1N1B4_1448 0.87 +0.1 1,662,153 + Pf1N1B4_1448 0.87 -0.8 1,662,153 + Pf1N1B4_1448 0.87 -2.0 1,662,153 + Pf1N1B4_1448 0.87 -1.0 1,662,154 - Pf1N1B4_1448 0.87 -0.5 1,662,154 - Pf1N1B4_1448 0.87 -2.0 1,662,154 - Pf1N1B4_1448 0.87 +1.6 1,662,154 - Pf1N1B4_1448 0.87 +1.6 1,662,175 - Pf1N1B4_1448 0.88 +0.7 1,662,175 - Pf1N1B4_1448 0.88 -1.4 1,662,175 - Pf1N1B4_1448 0.88 -0.0 1,662,264 + +0.3 1,662,264 + +0.4 1,662,342 + -0.3 1,662,343 - +1.0 1,662,425 - +0.7
Or see this region's nucleotide sequence