Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1294 and Pf1N1B4_1295 are separated by 24 nucleotides Pf1N1B4_1295 and Pf1N1B4_1296 are separated by 257 nucleotides Pf1N1B4_1296 and Pf1N1B4_1297 are separated by 222 nucleotides Pf1N1B4_1297 and Pf1N1B4_1298 are separated by 291 nucleotides
Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122
_1294
Pf1N1B4_1295 - hypothetical protein, at 1,476,147 to 1,476,368
_1295
Pf1N1B4_1296 - Biosynthetic arginine decarboxylase (EC 4.1.1.19) (from data) , at 1,476,626 to 1,478,539
_1296
Pf1N1B4_1297 - Translation initiation factor SUI1-related protein, at 1,478,762 to 1,479,133
_1297
Pf1N1B4_1298 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 1,479,425 to 1,479,994
_1298
Position (kb)
1476
1477
1478
1479 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1475.890 kb on + strand, within Pf1N1B4_1294 at 1476.030 kb on + strand at 1476.031 kb on - strand at 1476.031 kb on - strand at 1476.129 kb on + strand at 1476.130 kb on - strand at 1476.226 kb on + strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.227 kb on - strand, within Pf1N1B4_1295 at 1476.351 kb on + strand at 1476.351 kb on + strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.352 kb on - strand at 1476.449 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.462 kb on + strand at 1476.469 kb on + strand at 1476.470 kb on - strand at 1476.470 kb on - strand at 1476.481 kb on + strand at 1476.481 kb on + strand at 1476.482 kb on - strand at 1476.583 kb on + strand at 1476.584 kb on - strand at 1476.584 kb on - strand at 1476.622 kb on + strand at 1476.622 kb on + strand at 1476.623 kb on - strand at 1476.623 kb on - strand at 1476.639 kb on + strand at 1476.639 kb on + strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.640 kb on - strand at 1476.765 kb on + strand at 1476.813 kb on + strand at 1476.814 kb on - strand at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.837 kb on + strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.838 kb on - strand, within Pf1N1B4_1296 at 1476.927 kb on + strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.928 kb on - strand, within Pf1N1B4_1296 at 1476.954 kb on - strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.990 kb on + strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1476.991 kb on - strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.146 kb on + strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.147 kb on - strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.245 kb on + strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.246 kb on - strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.296 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.320 kb on + strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand, within Pf1N1B4_1296 at 1477.321 kb on - strand at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.338 kb on + strand, within Pf1N1B4_1296 at 1477.587 kb on + strand, within Pf1N1B4_1296 at 1477.602 kb on + strand, within Pf1N1B4_1296 at 1477.602 kb on + strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.603 kb on - strand, within Pf1N1B4_1296 at 1477.641 kb on + strand, within Pf1N1B4_1296 at 1477.642 kb on - strand, within Pf1N1B4_1296 at 1477.710 kb on + strand, within Pf1N1B4_1296 at 1477.710 kb on + strand, within Pf1N1B4_1296 at 1477.743 kb on + strand, within Pf1N1B4_1296 at 1477.743 kb on + strand, within Pf1N1B4_1296 at 1478.017 kb on + strand, within Pf1N1B4_1296 at 1478.018 kb on - strand, within Pf1N1B4_1296 at 1478.018 kb on - strand, within Pf1N1B4_1296 at 1478.232 kb on + strand, within Pf1N1B4_1296 at 1478.233 kb on - strand, within Pf1N1B4_1296 at 1478.292 kb on - strand, within Pf1N1B4_1296 at 1478.550 kb on + strand at 1478.552 kb on + strand at 1478.552 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.563 kb on + strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.564 kb on - strand at 1478.596 kb on + strand at 1478.596 kb on + strand at 1478.617 kb on - strand at 1478.617 kb on - strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.635 kb on + strand at 1478.636 kb on - strand at 1478.661 kb on - strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.696 kb on + strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1478.697 kb on - strand at 1479.300 kb on - strand at 1479.341 kb on - strand at 1479.343 kb on + strand at 1479.344 kb on - strand at 1479.344 kb on - strand at 1479.349 kb on + strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.350 kb on - strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.426 kb on + strand at 1479.427 kb on - strand at 1479.427 kb on - strand at 1479.428 kb on + strand at 1479.429 kb on - strand at 1479.429 kb on - strand at 1479.473 kb on + strand at 1479.473 kb on + strand at 1479.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 1,475,890 + Pf1N1B4_1294 0.78 -1.1 1,476,030 + +1.2 1,476,031 - -0.1 1,476,031 - -1.1 1,476,129 + +3.6 1,476,130 - -1.3 1,476,226 + Pf1N1B4_1295 0.36 -0.6 1,476,227 - Pf1N1B4_1295 0.36 -0.3 1,476,227 - Pf1N1B4_1295 0.36 -0.0 1,476,351 + +0.9 1,476,351 + +0.3 1,476,352 - -1.1 1,476,352 - -1.1 1,476,352 - -0.1 1,476,352 - +0.5 1,476,449 + +0.1 1,476,462 + -0.5 1,476,462 + -1.1 1,476,462 + +0.3 1,476,462 + -2.1 1,476,462 + -0.1 1,476,469 + +0.9 1,476,470 - -2.6 1,476,470 - +0.5 1,476,481 + +1.5 1,476,481 + -2.4 1,476,482 - -2.4 1,476,583 + +1.9 1,476,584 - -0.1 1,476,584 - -3.1 1,476,622 + -0.1 1,476,622 + -0.3 1,476,623 - -1.1 1,476,623 - +0.6 1,476,639 + -0.5 1,476,639 + -2.5 1,476,640 - +0.4 1,476,640 - -1.7 1,476,640 - -0.1 1,476,640 - -0.5 1,476,640 - -3.4 1,476,765 + -0.7 1,476,813 + -1.6 1,476,814 - -4.8 1,476,837 + Pf1N1B4_1296 0.11 -0.9 1,476,837 + Pf1N1B4_1296 0.11 +0.5 1,476,837 + Pf1N1B4_1296 0.11 -4.1 1,476,838 - Pf1N1B4_1296 0.11 -1.3 1,476,838 - Pf1N1B4_1296 0.11 -2.6 1,476,927 + Pf1N1B4_1296 0.16 -0.7 1,476,928 - Pf1N1B4_1296 0.16 -0.5 1,476,928 - Pf1N1B4_1296 0.16 -2.4 1,476,954 - Pf1N1B4_1296 0.17 -2.9 1,476,990 + Pf1N1B4_1296 0.19 -2.4 1,476,990 + Pf1N1B4_1296 0.19 -2.5 1,476,991 - Pf1N1B4_1296 0.19 -1.6 1,476,991 - Pf1N1B4_1296 0.19 -4.0 1,476,991 - Pf1N1B4_1296 0.19 -2.1 1,477,146 + Pf1N1B4_1296 0.27 -2.4 1,477,146 + Pf1N1B4_1296 0.27 -1.4 1,477,146 + Pf1N1B4_1296 0.27 -3.4 1,477,146 + Pf1N1B4_1296 0.27 -2.2 1,477,146 + Pf1N1B4_1296 0.27 -2.2 1,477,146 + Pf1N1B4_1296 0.27 -2.6 1,477,146 + Pf1N1B4_1296 0.27 -1.6 1,477,147 - Pf1N1B4_1296 0.27 -0.1 1,477,147 - Pf1N1B4_1296 0.27 +0.5 1,477,147 - Pf1N1B4_1296 0.27 -0.1 1,477,147 - Pf1N1B4_1296 0.27 -2.4 1,477,147 - Pf1N1B4_1296 0.27 -0.1 1,477,147 - Pf1N1B4_1296 0.27 -1.4 1,477,147 - Pf1N1B4_1296 0.27 -2.9 1,477,147 - Pf1N1B4_1296 0.27 -1.1 1,477,147 - Pf1N1B4_1296 0.27 -0.5 1,477,147 - Pf1N1B4_1296 0.27 +0.4 1,477,245 + Pf1N1B4_1296 0.32 -1.1 1,477,245 + Pf1N1B4_1296 0.32 -2.1 1,477,246 - Pf1N1B4_1296 0.32 +0.7 1,477,246 - Pf1N1B4_1296 0.32 -1.6 1,477,246 - Pf1N1B4_1296 0.32 -0.8 1,477,246 - Pf1N1B4_1296 0.32 -2.1 1,477,246 - Pf1N1B4_1296 0.32 -1.7 1,477,296 + Pf1N1B4_1296 0.35 -1.1 1,477,296 + Pf1N1B4_1296 0.35 -2.1 1,477,320 + Pf1N1B4_1296 0.36 -1.9 1,477,320 + -0.1 1,477,320 + Pf1N1B4_1296 0.36 -1.6 1,477,320 + Pf1N1B4_1296 0.36 -1.4 1,477,321 - Pf1N1B4_1296 0.36 -3.2 1,477,321 - Pf1N1B4_1296 0.36 -1.4 1,477,321 - Pf1N1B4_1296 0.36 -2.4 1,477,321 - Pf1N1B4_1296 0.36 -0.1 1,477,321 - Pf1N1B4_1296 0.36 -0.6 1,477,321 - -0.1 1,477,338 + Pf1N1B4_1296 0.37 -1.1 1,477,338 + Pf1N1B4_1296 0.37 -1.1 1,477,338 + Pf1N1B4_1296 0.37 -1.1 1,477,587 + Pf1N1B4_1296 0.50 -0.1 1,477,602 + Pf1N1B4_1296 0.51 -2.4 1,477,602 + Pf1N1B4_1296 0.51 -0.9 1,477,603 - Pf1N1B4_1296 0.51 -0.6 1,477,603 - Pf1N1B4_1296 0.51 -1.6 1,477,603 - Pf1N1B4_1296 0.51 +0.2 1,477,603 - Pf1N1B4_1296 0.51 -0.8 1,477,641 + Pf1N1B4_1296 0.53 -4.1 1,477,642 - Pf1N1B4_1296 0.53 -1.1 1,477,710 + Pf1N1B4_1296 0.57 -2.4 1,477,710 + Pf1N1B4_1296 0.57 -2.1 1,477,743 + Pf1N1B4_1296 0.58 -1.5 1,477,743 + Pf1N1B4_1296 0.58 -1.2 1,478,017 + Pf1N1B4_1296 0.73 -0.1 1,478,018 - Pf1N1B4_1296 0.73 +0.1 1,478,018 - Pf1N1B4_1296 0.73 -2.4 1,478,232 + Pf1N1B4_1296 0.84 -2.4 1,478,233 - Pf1N1B4_1296 0.84 +0.3 1,478,292 - Pf1N1B4_1296 0.87 -2.5 1,478,550 + -2.9 1,478,552 + +0.7 1,478,552 + -0.1 1,478,563 + -2.5 1,478,563 + -2.4 1,478,563 + -1.6 1,478,563 + -1.1 1,478,563 + -0.2 1,478,564 - -0.1 1,478,564 - -1.1 1,478,564 - +0.5 1,478,564 - -2.1 1,478,596 + +0.3 1,478,596 + -0.8 1,478,617 - -1.6 1,478,617 - +1.4 1,478,635 + -0.5 1,478,635 + -0.9 1,478,635 + +0.1 1,478,635 + -2.0 1,478,636 - +2.9 1,478,661 - -2.4 1,478,696 + -1.9 1,478,696 + -1.6 1,478,696 + -3.1 1,478,696 + +0.4 1,478,696 + +0.9 1,478,697 - +1.5 1,478,697 - -1.1 1,478,697 - -2.1 1,478,697 - +1.5 1,478,697 - -0.1 1,479,300 - -2.1 1,479,341 - +0.2 1,479,343 + -0.1 1,479,344 - -1.4 1,479,344 - +1.9 1,479,349 + +1.5 1,479,350 - +1.5 1,479,350 - -1.6 1,479,350 - -1.6 1,479,350 - -0.6 1,479,350 - +1.8 1,479,350 - -0.1 1,479,426 + -0.1 1,479,426 + -1.6 1,479,426 + -1.2 1,479,426 + -3.2 1,479,427 - +0.9 1,479,427 - -2.1 1,479,428 + -1.2 1,479,429 - -2.6 1,479,429 - -1.6 1,479,473 + -0.5 1,479,473 + -0.1 1,479,474 - +2.0
Or see this region's nucleotide sequence