Experiment: Deoxyribonucleic from herring sperm 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02228 and H281DRAFT_02229 overlap by 4 nucleotides H281DRAFT_02229 and H281DRAFT_02230 are separated by 203 nucleotides H281DRAFT_02230 and H281DRAFT_02231 are separated by 6 nucleotides H281DRAFT_02231 and H281DRAFT_02232 are separated by 72 nucleotides
H281DRAFT_02228: H281DRAFT_02228 - Sel1 repeat-containing protein, at 373,783 to 374,484
_02228
H281DRAFT_02229: H281DRAFT_02229 - 2-dehydro-3-deoxyglucarate aldolase, at 374,481 to 375,263
_02229
H281DRAFT_02230: H281DRAFT_02230 - 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase, at 375,467 to 375,913
_02230
H281DRAFT_02231: H281DRAFT_02231 - 7-carboxy-7-deazaguanine synthase, Cx14CxxC type, at 375,920 to 376,552
_02231
H281DRAFT_02232: H281DRAFT_02232 - preQ(0) biosynthesis protein QueC, at 376,625 to 377,356
_02232
Position (kb) 375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 374.553 kb on - strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.431 kb on + strand at 375.556 kb on - strand, within H281DRAFT_02230 at 375.822 kb on + strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.842 kb on - strand, within H281DRAFT_02230 at 375.936 kb on + strand at 375.936 kb on + strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 376.048 kb on - strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.522 kb on + strand at 376.522 kb on + strand at 376.522 kb on + strand at 376.523 kb on - strand at 376.523 kb on - strand at 376.524 kb on + strand at 376.525 kb on - strand at 376.574 kb on + strand at 376.749 kb on + strand, within H281DRAFT_02232 at 376.750 kb on - strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.766 kb on + strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.799 kb on + strand, within H281DRAFT_02232 at 376.800 kb on - strand, within H281DRAFT_02232 at 376.822 kb on + strand, within H281DRAFT_02232
Per-strain Table
Position Strand Gene LocusTag Fraction Deoxyribonucleic from herring sperm 10 mg/mL (C) remove 374,553 - -0.7 375,420 + -0.6 375,420 + +0.1 375,420 + -0.9 375,420 + +0.8 375,431 + +0.8 375,556 - H281DRAFT_02230 0.20 -2.4 375,822 + H281DRAFT_02230 0.79 -3.1 375,823 - H281DRAFT_02230 0.80 +1.9 375,823 - H281DRAFT_02230 0.80 -1.6 375,823 - H281DRAFT_02230 0.80 -0.3 375,823 - H281DRAFT_02230 0.80 -0.8 375,842 - H281DRAFT_02230 0.84 -2.3 375,936 + -0.7 375,936 + -0.3 375,937 - -1.3 375,937 - -1.0 375,937 - -1.5 375,937 - -0.3 375,937 - -0.9 376,048 - H281DRAFT_02231 0.20 -2.4 376,244 + H281DRAFT_02231 0.51 -2.0 376,244 + H281DRAFT_02231 0.51 -1.0 376,522 + -2.5 376,522 + -1.5 376,522 + -1.1 376,523 - -0.2 376,523 - -2.3 376,524 + -1.7 376,525 - -1.0 376,574 + -1.0 376,749 + H281DRAFT_02232 0.17 +0.3 376,750 - H281DRAFT_02232 0.17 +0.5 376,756 + H281DRAFT_02232 0.18 -0.2 376,756 + H281DRAFT_02232 0.18 -1.5 376,756 + H281DRAFT_02232 0.18 +0.9 376,766 + H281DRAFT_02232 0.19 -0.1 376,767 - H281DRAFT_02232 0.19 -1.4 376,767 - H281DRAFT_02232 0.19 -0.7 376,767 - H281DRAFT_02232 0.19 -0.8 376,767 - H281DRAFT_02232 0.19 +0.3 376,799 + H281DRAFT_02232 0.24 -0.9 376,800 - H281DRAFT_02232 0.24 -2.0 376,822 + H281DRAFT_02232 0.27 -1.0
Or see this region's nucleotide sequence