Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3503 and Dsui_3504 are separated by 12 nucleotides Dsui_3504 and Dsui_3505 overlap by 1 nucleotides Dsui_3505 and Dsui_3506 are separated by 93 nucleotides
Dsui_3503: Dsui_3503 - protein of unknown function (DUF1992), at 3,769,226 to 3,769,651
_3503
Dsui_3504: Dsui_3504 - rhodanese-related sulfurtransferase, at 3,769,664 to 3,770,512
_3504
Dsui_3505: Dsui_3505 - Protein of unknown function (DUF3478), at 3,770,512 to 3,770,868
_3505
Dsui_3506: Dsui_3506 - putative permease, DMT superfamily, at 3,770,962 to 3,771,819
_3506
Position (kb)
3770
3771 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3769.514 kb on + strand at 3769.563 kb on - strand, within Dsui_3503 at 3769.596 kb on + strand, within Dsui_3503 at 3769.614 kb on - strand at 3769.643 kb on + strand at 3769.643 kb on + strand at 3769.716 kb on + strand at 3769.804 kb on - strand, within Dsui_3504 at 3769.828 kb on + strand, within Dsui_3504 at 3769.831 kb on + strand at 3769.889 kb on - strand, within Dsui_3504 at 3770.225 kb on + strand, within Dsui_3504 at 3770.376 kb on + strand at 3770.411 kb on + strand, within Dsui_3504 at 3770.478 kb on - strand at 3770.616 kb on - strand, within Dsui_3505 at 3770.627 kb on - strand, within Dsui_3505 at 3770.669 kb on + strand, within Dsui_3505 at 3770.709 kb on - strand, within Dsui_3505 at 3770.720 kb on + strand at 3770.784 kb on + strand, within Dsui_3505 at 3770.793 kb on + strand, within Dsui_3505 at 3770.801 kb on - strand, within Dsui_3505 at 3770.801 kb on - strand, within Dsui_3505 at 3770.839 kb on - strand at 3770.845 kb on + strand at 3770.871 kb on - strand at 3770.872 kb on - strand at 3770.877 kb on - strand at 3770.877 kb on - strand at 3770.877 kb on - strand at 3770.877 kb on - strand at 3770.877 kb on - strand at 3770.928 kb on + strand at 3770.936 kb on - strand at 3770.960 kb on + strand at 3770.960 kb on + strand at 3770.991 kb on - strand at 3771.147 kb on - strand, within Dsui_3506 at 3771.180 kb on - strand, within Dsui_3506 at 3771.207 kb on + strand, within Dsui_3506 at 3771.297 kb on - strand, within Dsui_3506 at 3771.297 kb on - strand, within Dsui_3506 at 3771.319 kb on + strand, within Dsui_3506 at 3771.324 kb on + strand at 3771.347 kb on - strand, within Dsui_3506 at 3771.441 kb on + strand at 3771.493 kb on + strand, within Dsui_3506 at 3771.523 kb on - strand, within Dsui_3506 at 3771.617 kb on + strand at 3771.652 kb on - strand, within Dsui_3506 at 3771.654 kb on + strand at 3771.662 kb on + strand at 3771.666 kb on + strand at 3771.673 kb on - strand, within Dsui_3506 at 3771.690 kb on + strand, within Dsui_3506 at 3771.729 kb on + strand at 3771.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,769,514 + -0.9 3,769,563 - Dsui_3503 0.79 +0.4 3,769,596 + Dsui_3503 0.87 +1.2 3,769,614 - +0.2 3,769,643 + +0.1 3,769,643 + -2.2 3,769,716 + +1.0 3,769,804 - Dsui_3504 0.16 +0.9 3,769,828 + Dsui_3504 0.19 -0.8 3,769,831 + +1.4 3,769,889 - Dsui_3504 0.27 +1.4 3,770,225 + Dsui_3504 0.66 -0.0 3,770,376 + +0.1 3,770,411 + Dsui_3504 0.88 -1.4 3,770,478 - +1.0 3,770,616 - Dsui_3505 0.29 +1.3 3,770,627 - Dsui_3505 0.32 +1.4 3,770,669 + Dsui_3505 0.44 -0.9 3,770,709 - Dsui_3505 0.55 +0.4 3,770,720 + -2.3 3,770,784 + Dsui_3505 0.76 -0.4 3,770,793 + Dsui_3505 0.79 +0.4 3,770,801 - Dsui_3505 0.81 +1.0 3,770,801 - Dsui_3505 0.81 +1.2 3,770,839 - +1.4 3,770,845 + +1.5 3,770,871 - -0.7 3,770,872 - +1.9 3,770,877 - +0.2 3,770,877 - +1.0 3,770,877 - +0.1 3,770,877 - +0.7 3,770,877 - +0.7 3,770,928 + -1.9 3,770,936 - +1.2 3,770,960 + -2.5 3,770,960 + -1.7 3,770,991 - -3.1 3,771,147 - Dsui_3506 0.22 -1.9 3,771,180 - Dsui_3506 0.25 -3.6 3,771,207 + Dsui_3506 0.29 -6.0 3,771,297 - Dsui_3506 0.39 -3.2 3,771,297 - Dsui_3506 0.39 -2.3 3,771,319 + Dsui_3506 0.42 -2.8 3,771,324 + -0.3 3,771,347 - Dsui_3506 0.45 -3.5 3,771,441 + +0.3 3,771,493 + Dsui_3506 0.62 -4.1 3,771,523 - Dsui_3506 0.65 -2.4 3,771,617 + -2.7 3,771,652 - Dsui_3506 0.80 -4.2 3,771,654 + -2.3 3,771,662 + -1.5 3,771,666 + -2.3 3,771,673 - Dsui_3506 0.83 -3.8 3,771,690 + Dsui_3506 0.85 -4.5 3,771,729 + -3.2 3,771,776 - -2.4
Or see this region's nucleotide sequence