Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3027 and Dsui_3028 are separated by 12 nucleotides Dsui_3028 and Dsui_3029 overlap by 4 nucleotides Dsui_3029 and Dsui_3030 overlap by 4 nucleotides
Dsui_3027: Dsui_3027 - coenzyme F420-reducing hydrogenase, alpha subunit, at 3,238,215 to 3,239,675
_3027
Dsui_3028: Dsui_3028 - coenzyme F420-reducing hydrogenase, gamma subunit, at 3,239,688 to 3,240,245
_3028
Dsui_3029: Dsui_3029 - NADH:ubiquinone oxidoreductase chain G-like protein, at 3,240,242 to 3,240,955
_3029
Dsui_3030: Dsui_3030 - NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit, at 3,240,952 to 3,242,760
_3030
Position (kb)
3240
3241 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3239.259 kb on - strand, within Dsui_3027 at 3239.417 kb on - strand, within Dsui_3027 at 3239.599 kb on + strand at 3239.600 kb on - strand at 3239.832 kb on - strand, within Dsui_3028 at 3239.913 kb on - strand, within Dsui_3028 at 3239.939 kb on - strand, within Dsui_3028 at 3240.113 kb on - strand, within Dsui_3028 at 3240.364 kb on - strand, within Dsui_3029 at 3240.414 kb on + strand, within Dsui_3029 at 3240.695 kb on - strand, within Dsui_3029 at 3240.726 kb on + strand at 3240.979 kb on - strand at 3241.046 kb on - strand at 3241.142 kb on - strand, within Dsui_3030 at 3241.583 kb on - strand, within Dsui_3030 at 3241.612 kb on - strand, within Dsui_3030 at 3241.861 kb on + strand at 3241.868 kb on + strand at 3241.879 kb on - strand, within Dsui_3030 at 3241.929 kb on - strand, within Dsui_3030
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,239,259 - Dsui_3027 0.71 -1.0 3,239,417 - Dsui_3027 0.82 +1.4 3,239,599 + -2.4 3,239,600 - +0.5 3,239,832 - Dsui_3028 0.26 -1.0 3,239,913 - Dsui_3028 0.40 +0.6 3,239,939 - Dsui_3028 0.45 -0.1 3,240,113 - Dsui_3028 0.76 +0.5 3,240,364 - Dsui_3029 0.17 +0.6 3,240,414 + Dsui_3029 0.24 -3.4 3,240,695 - Dsui_3029 0.63 +1.0 3,240,726 + -2.5 3,240,979 - -1.0 3,241,046 - -2.5 3,241,142 - Dsui_3030 0.11 -0.7 3,241,583 - Dsui_3030 0.35 -0.0 3,241,612 - Dsui_3030 0.36 -1.9 3,241,861 + -3.5 3,241,868 + -2.2 3,241,879 - Dsui_3030 0.51 -0.7 3,241,929 - Dsui_3030 0.54 -1.7
Or see this region's nucleotide sequence