Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2944 and Dsui_2945 are separated by 313 nucleotides Dsui_2945 and Dsui_2946 overlap by 1 nucleotides Dsui_2946 and Dsui_2947 overlap by 4 nucleotides Dsui_2947 and Dsui_2948 are separated by 170 nucleotides
Dsui_2944: Dsui_2944 - diguanylate cyclase (GGDEF) domain-containing protein, at 3,149,972 to 3,151,684
_2944
Dsui_2945: Dsui_2945 - hypothetical protein, at 3,151,998 to 3,152,486
_2945
Dsui_2946: Dsui_2946 - DNA/RNA helicase, superfamily II, at 3,152,486 to 3,153,916
_2946
Dsui_2947: Dsui_2947 - putative metal-dependent hydrolase, at 3,153,913 to 3,154,479
_2947
Dsui_2948: Dsui_2948 - RNA polymerase sigma factor, sigma-70 family, at 3,154,650 to 3,155,147
_2948
Position (kb)
3152
3153
3154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3151.557 kb on + strand at 3151.617 kb on - strand at 3151.629 kb on - strand at 3151.786 kb on - strand at 3151.811 kb on + strand at 3151.870 kb on - strand at 3151.907 kb on - strand at 3151.950 kb on - strand at 3151.955 kb on + strand at 3151.963 kb on + strand at 3151.975 kb on + strand at 3151.983 kb on - strand at 3152.125 kb on + strand, within Dsui_2945 at 3152.174 kb on - strand at 3152.347 kb on - strand at 3152.353 kb on + strand, within Dsui_2945 at 3152.372 kb on + strand, within Dsui_2945 at 3152.407 kb on + strand, within Dsui_2945 at 3152.408 kb on + strand, within Dsui_2945 at 3152.408 kb on + strand, within Dsui_2945 at 3152.437 kb on - strand at 3152.658 kb on + strand, within Dsui_2946 at 3152.712 kb on + strand, within Dsui_2946 at 3152.712 kb on + strand, within Dsui_2946 at 3152.730 kb on - strand at 3152.767 kb on + strand, within Dsui_2946 at 3152.778 kb on + strand, within Dsui_2946 at 3152.810 kb on - strand at 3152.961 kb on + strand, within Dsui_2946 at 3152.965 kb on + strand, within Dsui_2946 at 3152.993 kb on - strand at 3153.070 kb on + strand, within Dsui_2946 at 3153.308 kb on - strand, within Dsui_2946 at 3153.308 kb on - strand at 3153.308 kb on - strand at 3153.563 kb on - strand, within Dsui_2946 at 3153.693 kb on + strand, within Dsui_2946 at 3153.819 kb on + strand at 3153.882 kb on - strand at 3153.981 kb on - strand, within Dsui_2947 at 3154.021 kb on + strand, within Dsui_2947 at 3154.079 kb on + strand, within Dsui_2947 at 3154.195 kb on - strand, within Dsui_2947 at 3154.233 kb on + strand, within Dsui_2947 at 3154.282 kb on + strand, within Dsui_2947 at 3154.315 kb on + strand, within Dsui_2947 at 3154.323 kb on - strand, within Dsui_2947 at 3154.394 kb on - strand at 3154.540 kb on - strand at 3154.540 kb on - strand at 3154.601 kb on + strand at 3154.609 kb on - strand at 3154.653 kb on + strand at 3154.690 kb on - strand at 3154.690 kb on - strand at 3154.690 kb on - strand at 3154.824 kb on - strand, within Dsui_2948 at 3154.859 kb on - strand at 3154.889 kb on - strand, within Dsui_2948 at 3154.894 kb on - strand, within Dsui_2948 at 3154.899 kb on + strand, within Dsui_2948
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,151,557 + +1.4 3,151,617 - -0.3 3,151,629 - +1.9 3,151,786 - +0.8 3,151,811 + -0.2 3,151,870 - -2.8 3,151,907 - +1.0 3,151,950 - -2.0 3,151,955 + +0.4 3,151,963 + +1.4 3,151,975 + +0.4 3,151,983 - +0.5 3,152,125 + Dsui_2945 0.26 +0.0 3,152,174 - +1.1 3,152,347 - -1.0 3,152,353 + Dsui_2945 0.73 +1.1 3,152,372 + Dsui_2945 0.76 -1.5 3,152,407 + Dsui_2945 0.84 +0.8 3,152,408 + Dsui_2945 0.84 +0.8 3,152,408 + Dsui_2945 0.84 +2.0 3,152,437 - +0.9 3,152,658 + Dsui_2946 0.12 -0.9 3,152,712 + Dsui_2946 0.16 +1.0 3,152,712 + Dsui_2946 0.16 +1.2 3,152,730 - -0.5 3,152,767 + Dsui_2946 0.20 +1.0 3,152,778 + Dsui_2946 0.20 +2.3 3,152,810 - -0.3 3,152,961 + Dsui_2946 0.33 +0.8 3,152,965 + Dsui_2946 0.33 +0.7 3,152,993 - -1.4 3,153,070 + Dsui_2946 0.41 -0.4 3,153,308 - Dsui_2946 0.57 +0.6 3,153,308 - -1.2 3,153,308 - -2.1 3,153,563 - Dsui_2946 0.75 +0.8 3,153,693 + Dsui_2946 0.84 -0.4 3,153,819 + -0.6 3,153,882 - -0.7 3,153,981 - Dsui_2947 0.12 +0.0 3,154,021 + Dsui_2947 0.19 +0.4 3,154,079 + Dsui_2947 0.29 +0.7 3,154,195 - Dsui_2947 0.50 -2.0 3,154,233 + Dsui_2947 0.56 +0.0 3,154,282 + Dsui_2947 0.65 +0.7 3,154,315 + Dsui_2947 0.71 +0.1 3,154,323 - Dsui_2947 0.72 +0.0 3,154,394 - +0.1 3,154,540 - +0.1 3,154,540 - -0.9 3,154,601 + +1.5 3,154,609 - -0.0 3,154,653 + +0.9 3,154,690 - -2.9 3,154,690 - +0.6 3,154,690 - -1.4 3,154,824 - Dsui_2948 0.35 -3.4 3,154,859 - -2.1 3,154,889 - Dsui_2948 0.48 -0.0 3,154,894 - Dsui_2948 0.49 -0.7 3,154,899 + Dsui_2948 0.50 +0.5
Or see this region's nucleotide sequence