Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2548 and Dsui_2549 are separated by 40 nucleotides Dsui_2549 and Dsui_2550 are separated by 134 nucleotides Dsui_2550 and Dsui_2551 are separated by 67 nucleotides Dsui_2551 and Dsui_2552 are separated by 172 nucleotides Dsui_2552 and Dsui_2553 overlap by 4 nucleotides
Dsui_2548: Dsui_2548 - hypothetical protein, at 2,702,078 to 2,702,518
_2548
Dsui_2549: Dsui_2549 - hypothetical protein, at 2,702,559 to 2,702,996
_2549
Dsui_2550: Dsui_2550 - Protein of unknown function (DUF2726), at 2,703,131 to 2,703,694
_2550
Dsui_2551: Dsui_2551 - Helix-destabilising protein, at 2,703,762 to 2,704,055
_2551
Dsui_2552: Dsui_2552 - hypothetical protein, at 2,704,228 to 2,704,503
_2552
Dsui_2553: Dsui_2553 - Phage X family/Phage replication protein CRI, at 2,704,500 to 2,705,582
_2553
Position (kb)
2703
2704 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2702.206 kb on - strand, within Dsui_2548 at 2702.225 kb on - strand, within Dsui_2548 at 2702.252 kb on + strand, within Dsui_2548 at 2702.387 kb on + strand, within Dsui_2548 at 2702.445 kb on - strand, within Dsui_2548 at 2702.646 kb on + strand, within Dsui_2549 at 2702.749 kb on + strand, within Dsui_2549 at 2702.803 kb on - strand, within Dsui_2549 at 2702.804 kb on - strand, within Dsui_2549 at 2702.830 kb on + strand, within Dsui_2549 at 2702.830 kb on + strand at 2702.830 kb on + strand, within Dsui_2549 at 2702.835 kb on - strand, within Dsui_2549 at 2702.838 kb on - strand, within Dsui_2549 at 2702.838 kb on - strand, within Dsui_2549 at 2702.838 kb on - strand, within Dsui_2549 at 2702.838 kb on - strand, within Dsui_2549 at 2702.882 kb on - strand, within Dsui_2549 at 2702.915 kb on + strand, within Dsui_2549 at 2702.979 kb on + strand at 2703.100 kb on + strand at 2703.100 kb on - strand at 2703.104 kb on + strand at 2703.104 kb on + strand at 2703.104 kb on + strand at 2703.122 kb on - strand at 2703.158 kb on - strand at 2703.203 kb on - strand, within Dsui_2550 at 2703.222 kb on + strand, within Dsui_2550 at 2703.222 kb on + strand, within Dsui_2550 at 2703.230 kb on - strand, within Dsui_2550 at 2703.240 kb on + strand at 2703.248 kb on - strand, within Dsui_2550 at 2703.248 kb on - strand, within Dsui_2550 at 2703.294 kb on - strand, within Dsui_2550 at 2703.294 kb on - strand, within Dsui_2550 at 2703.313 kb on + strand, within Dsui_2550 at 2703.325 kb on + strand at 2703.325 kb on + strand at 2703.328 kb on - strand, within Dsui_2550 at 2703.328 kb on - strand, within Dsui_2550 at 2703.335 kb on + strand, within Dsui_2550 at 2703.338 kb on - strand, within Dsui_2550 at 2703.340 kb on + strand, within Dsui_2550 at 2703.343 kb on - strand, within Dsui_2550 at 2703.343 kb on - strand, within Dsui_2550 at 2703.348 kb on + strand at 2703.348 kb on - strand, within Dsui_2550 at 2703.399 kb on + strand at 2703.407 kb on - strand, within Dsui_2550 at 2703.455 kb on - strand, within Dsui_2550 at 2703.455 kb on - strand, within Dsui_2550 at 2703.465 kb on - strand, within Dsui_2550 at 2703.465 kb on - strand, within Dsui_2550 at 2703.465 kb on - strand, within Dsui_2550 at 2703.542 kb on - strand, within Dsui_2550 at 2703.605 kb on - strand, within Dsui_2550 at 2703.786 kb on + strand at 2703.794 kb on - strand, within Dsui_2551 at 2703.794 kb on - strand, within Dsui_2551 at 2703.794 kb on - strand, within Dsui_2551 at 2703.830 kb on + strand at 2703.838 kb on - strand, within Dsui_2551 at 2703.876 kb on - strand, within Dsui_2551 at 2703.946 kb on - strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.955 kb on + strand, within Dsui_2551 at 2703.963 kb on - strand, within Dsui_2551 at 2703.963 kb on - strand, within Dsui_2551 at 2704.040 kb on + strand at 2704.048 kb on - strand at 2704.065 kb on - strand at 2704.065 kb on - strand at 2704.065 kb on - strand at 2704.145 kb on - strand at 2704.248 kb on + strand at 2704.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,702,206 - Dsui_2548 0.29 +1.7 2,702,225 - Dsui_2548 0.33 +1.1 2,702,252 + Dsui_2548 0.39 +0.5 2,702,387 + Dsui_2548 0.70 +1.2 2,702,445 - Dsui_2548 0.83 +1.6 2,702,646 + Dsui_2549 0.20 +0.2 2,702,749 + Dsui_2549 0.43 -2.5 2,702,803 - Dsui_2549 0.56 -0.2 2,702,804 - Dsui_2549 0.56 -1.1 2,702,830 + Dsui_2549 0.62 -1.9 2,702,830 + +1.5 2,702,830 + Dsui_2549 0.62 -1.2 2,702,835 - Dsui_2549 0.63 +0.1 2,702,838 - Dsui_2549 0.64 -1.3 2,702,838 - Dsui_2549 0.64 -1.3 2,702,838 - Dsui_2549 0.64 -1.3 2,702,838 - Dsui_2549 0.64 -0.1 2,702,882 - Dsui_2549 0.74 -1.0 2,702,915 + Dsui_2549 0.81 -2.4 2,702,979 + -0.5 2,703,100 + +2.4 2,703,100 - +1.3 2,703,104 + +1.5 2,703,104 + +1.0 2,703,104 + +0.7 2,703,122 - -2.4 2,703,158 - +0.5 2,703,203 - Dsui_2550 0.13 -2.1 2,703,222 + Dsui_2550 0.16 +1.5 2,703,222 + Dsui_2550 0.16 +1.5 2,703,230 - Dsui_2550 0.18 -2.9 2,703,240 + -0.9 2,703,248 - Dsui_2550 0.21 +0.3 2,703,248 - Dsui_2550 0.21 -2.4 2,703,294 - Dsui_2550 0.29 +2.0 2,703,294 - Dsui_2550 0.29 +1.9 2,703,313 + Dsui_2550 0.32 -2.2 2,703,325 + +1.5 2,703,325 + -1.5 2,703,328 - Dsui_2550 0.35 +0.1 2,703,328 - Dsui_2550 0.35 +0.8 2,703,335 + Dsui_2550 0.36 +2.5 2,703,338 - Dsui_2550 0.37 -3.1 2,703,340 + Dsui_2550 0.37 -3.1 2,703,343 - Dsui_2550 0.38 -3.0 2,703,343 - Dsui_2550 0.38 -0.9 2,703,348 + +0.6 2,703,348 - Dsui_2550 0.38 -0.9 2,703,399 + -1.4 2,703,407 - Dsui_2550 0.49 +0.9 2,703,455 - Dsui_2550 0.57 -2.9 2,703,455 - Dsui_2550 0.57 -1.5 2,703,465 - Dsui_2550 0.59 -0.1 2,703,465 - Dsui_2550 0.59 -1.9 2,703,465 - Dsui_2550 0.59 +0.4 2,703,542 - Dsui_2550 0.73 -2.1 2,703,605 - Dsui_2550 0.84 +1.0 2,703,786 + -2.2 2,703,794 - Dsui_2551 0.11 -0.0 2,703,794 - Dsui_2551 0.11 -0.1 2,703,794 - Dsui_2551 0.11 -0.3 2,703,830 + -1.4 2,703,838 - Dsui_2551 0.26 +0.6 2,703,876 - Dsui_2551 0.39 -0.6 2,703,946 - Dsui_2551 0.63 -0.2 2,703,955 + Dsui_2551 0.66 -1.5 2,703,955 + Dsui_2551 0.66 -2.1 2,703,955 + Dsui_2551 0.66 -1.5 2,703,963 - Dsui_2551 0.68 +0.6 2,703,963 - Dsui_2551 0.68 -1.5 2,704,040 + -1.9 2,704,048 - +2.5 2,704,065 - -2.1 2,704,065 - -2.9 2,704,065 - -0.6 2,704,145 - +0.2 2,704,248 + -2.5 2,704,606 - -1.5
Or see this region's nucleotide sequence