Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2544 and Dsui_2545 are separated by 14 nucleotides Dsui_2545 and Dsui_2546 are separated by 27 nucleotides Dsui_2546 and Dsui_2547 are separated by 250 nucleotides
Dsui_2544: Dsui_2544 - disulfide bond chaperone, at 2,695,537 to 2,696,394
_2544
Dsui_2545: Dsui_2545 - transcriptional regulator, Spx/MgsR family, at 2,696,409 to 2,696,756
_2545
Dsui_2546: Dsui_2546 - transcriptional accessory protein, at 2,696,784 to 2,699,099
_2546
Dsui_2547: Dsui_2547 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 2,699,350 to 2,702,019
_2547
Position (kb)
2696
2697
2698
2699
2700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2695.865 kb on + strand at 2695.919 kb on - strand, within Dsui_2544 at 2696.053 kb on + strand, within Dsui_2544 at 2696.120 kb on + strand, within Dsui_2544 at 2696.204 kb on - strand, within Dsui_2544 at 2696.379 kb on + strand at 2696.503 kb on - strand, within Dsui_2545 at 2696.532 kb on - strand, within Dsui_2545 at 2696.589 kb on - strand, within Dsui_2545 at 2696.693 kb on + strand, within Dsui_2545 at 2696.741 kb on + strand at 2696.746 kb on + strand at 2696.746 kb on + strand at 2696.747 kb on + strand at 2696.804 kb on + strand at 2697.003 kb on - strand at 2697.088 kb on - strand, within Dsui_2546 at 2697.242 kb on - strand, within Dsui_2546 at 2697.289 kb on + strand, within Dsui_2546 at 2697.370 kb on + strand, within Dsui_2546 at 2697.448 kb on - strand, within Dsui_2546 at 2697.593 kb on - strand, within Dsui_2546 at 2697.623 kb on + strand, within Dsui_2546 at 2697.626 kb on - strand, within Dsui_2546 at 2697.626 kb on - strand, within Dsui_2546 at 2697.626 kb on - strand, within Dsui_2546 at 2697.641 kb on - strand, within Dsui_2546 at 2697.850 kb on - strand, within Dsui_2546 at 2697.852 kb on + strand, within Dsui_2546 at 2697.852 kb on + strand, within Dsui_2546 at 2697.992 kb on + strand, within Dsui_2546 at 2698.033 kb on + strand, within Dsui_2546 at 2698.078 kb on - strand, within Dsui_2546 at 2698.298 kb on - strand, within Dsui_2546 at 2698.298 kb on - strand, within Dsui_2546 at 2698.378 kb on - strand, within Dsui_2546 at 2698.378 kb on - strand, within Dsui_2546 at 2698.378 kb on - strand, within Dsui_2546 at 2698.402 kb on - strand, within Dsui_2546 at 2698.621 kb on - strand, within Dsui_2546 at 2698.633 kb on + strand, within Dsui_2546 at 2698.633 kb on + strand, within Dsui_2546 at 2698.633 kb on + strand, within Dsui_2546 at 2698.641 kb on - strand, within Dsui_2546 at 2698.641 kb on - strand, within Dsui_2546 at 2698.641 kb on - strand, within Dsui_2546 at 2698.761 kb on - strand, within Dsui_2546 at 2698.790 kb on + strand, within Dsui_2546 at 2698.954 kb on - strand at 2699.043 kb on - strand at 2699.094 kb on - strand at 2699.207 kb on + strand at 2699.347 kb on + strand at 2699.421 kb on + strand at 2699.446 kb on - strand at 2699.446 kb on - strand at 2699.446 kb on - strand at 2699.456 kb on - strand at 2699.474 kb on + strand at 2699.510 kb on + strand at 2699.549 kb on + strand at 2699.600 kb on - strand at 2699.624 kb on + strand, within Dsui_2547 at 2699.634 kb on + strand, within Dsui_2547 at 2699.669 kb on + strand, within Dsui_2547 at 2699.821 kb on + strand, within Dsui_2547 at 2699.846 kb on + strand, within Dsui_2547 at 2699.859 kb on + strand, within Dsui_2547 at 2699.882 kb on + strand, within Dsui_2547 at 2699.886 kb on + strand, within Dsui_2547 at 2699.886 kb on + strand, within Dsui_2547 at 2699.887 kb on + strand, within Dsui_2547 at 2699.890 kb on - strand, within Dsui_2547 at 2699.897 kb on + strand, within Dsui_2547 at 2699.939 kb on + strand, within Dsui_2547 at 2700.021 kb on + strand, within Dsui_2547 at 2700.022 kb on + strand, within Dsui_2547
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,695,865 + +1.4 2,695,919 - Dsui_2544 0.45 +0.8 2,696,053 + Dsui_2544 0.60 +0.4 2,696,120 + Dsui_2544 0.68 +1.7 2,696,204 - Dsui_2544 0.78 +2.0 2,696,379 + +0.8 2,696,503 - Dsui_2545 0.27 +1.1 2,696,532 - Dsui_2545 0.35 +1.9 2,696,589 - Dsui_2545 0.52 +1.0 2,696,693 + Dsui_2545 0.82 +1.7 2,696,741 + +0.6 2,696,746 + +1.2 2,696,746 + +1.0 2,696,747 + -0.9 2,696,804 + +0.1 2,697,003 - +0.1 2,697,088 - Dsui_2546 0.13 +0.4 2,697,242 - Dsui_2546 0.20 +0.3 2,697,289 + Dsui_2546 0.22 -0.6 2,697,370 + Dsui_2546 0.25 -1.0 2,697,448 - Dsui_2546 0.29 -0.6 2,697,593 - Dsui_2546 0.35 +0.1 2,697,623 + Dsui_2546 0.36 -0.3 2,697,626 - Dsui_2546 0.36 -3.1 2,697,626 - Dsui_2546 0.36 -0.3 2,697,626 - Dsui_2546 0.36 -1.1 2,697,641 - Dsui_2546 0.37 +2.8 2,697,850 - Dsui_2546 0.46 -2.9 2,697,852 + Dsui_2546 0.46 -3.7 2,697,852 + Dsui_2546 0.46 -2.4 2,697,992 + Dsui_2546 0.52 -2.1 2,698,033 + Dsui_2546 0.54 -1.2 2,698,078 - Dsui_2546 0.56 +0.0 2,698,298 - Dsui_2546 0.65 +1.1 2,698,298 - Dsui_2546 0.65 +0.7 2,698,378 - Dsui_2546 0.69 -0.6 2,698,378 - Dsui_2546 0.69 -0.4 2,698,378 - Dsui_2546 0.69 +0.1 2,698,402 - Dsui_2546 0.70 +0.2 2,698,621 - Dsui_2546 0.79 -0.9 2,698,633 + Dsui_2546 0.80 -1.2 2,698,633 + Dsui_2546 0.80 +0.4 2,698,633 + Dsui_2546 0.80 -0.6 2,698,641 - Dsui_2546 0.80 -0.2 2,698,641 - Dsui_2546 0.80 +0.2 2,698,641 - Dsui_2546 0.80 +0.7 2,698,761 - Dsui_2546 0.85 -2.0 2,698,790 + Dsui_2546 0.87 -2.4 2,698,954 - +0.2 2,699,043 - +2.0 2,699,094 - +0.1 2,699,207 + -1.9 2,699,347 + -3.2 2,699,421 + +2.1 2,699,446 - -3.2 2,699,446 - -3.1 2,699,446 - -3.3 2,699,456 - -1.4 2,699,474 + +0.1 2,699,510 + +0.9 2,699,549 + +0.3 2,699,600 - -0.2 2,699,624 + Dsui_2547 0.10 +0.5 2,699,634 + Dsui_2547 0.11 +2.8 2,699,669 + Dsui_2547 0.12 +1.0 2,699,821 + Dsui_2547 0.18 -0.3 2,699,846 + Dsui_2547 0.19 +0.5 2,699,859 + Dsui_2547 0.19 -0.3 2,699,882 + Dsui_2547 0.20 +0.4 2,699,886 + Dsui_2547 0.20 +1.0 2,699,886 + Dsui_2547 0.20 +1.0 2,699,887 + Dsui_2547 0.20 +0.2 2,699,890 - Dsui_2547 0.20 -2.4 2,699,897 + Dsui_2547 0.20 +0.4 2,699,939 + Dsui_2547 0.22 +0.2 2,700,021 + Dsui_2547 0.25 +0.7 2,700,022 + Dsui_2547 0.25 +1.1
Or see this region's nucleotide sequence