Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2365 and Dsui_2366 are separated by 84 nucleotides Dsui_2366 and Dsui_2367 are separated by 101 nucleotides Dsui_2367 and Dsui_2368 are separated by 54 nucleotides
Dsui_2365: Dsui_2365 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 2,514,708 to 2,517,632
_2365
Dsui_2366: Dsui_2366 - inositol monophosphatase/fructose-1,6-bisphosphatase family protein, at 2,517,717 to 2,518,520
_2366
Dsui_2367: Dsui_2367 - RNA methyltransferase, TrmH family, group 1, at 2,518,622 to 2,519,401
_2367
Dsui_2368: Dsui_2368 - serine O-acetyltransferase, at 2,519,456 to 2,520,211
_2368
Position (kb)
2517
2518
2519 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2516.833 kb on - strand at 2516.930 kb on - strand at 2516.993 kb on + strand, within Dsui_2365 at 2517.276 kb on + strand, within Dsui_2365 at 2517.277 kb on + strand, within Dsui_2365 at 2517.304 kb on + strand, within Dsui_2365 at 2517.323 kb on + strand, within Dsui_2365 at 2517.350 kb on - strand at 2517.350 kb on - strand at 2517.512 kb on - strand at 2517.527 kb on + strand at 2517.535 kb on - strand at 2517.762 kb on - strand at 2517.762 kb on - strand at 2518.638 kb on + strand at 2518.648 kb on + strand at 2518.680 kb on - strand at 2518.725 kb on + strand, within Dsui_2367 at 2518.730 kb on + strand, within Dsui_2367 at 2518.740 kb on + strand, within Dsui_2367 at 2518.839 kb on + strand, within Dsui_2367 at 2518.884 kb on + strand, within Dsui_2367 at 2518.968 kb on + strand, within Dsui_2367 at 2518.979 kb on + strand, within Dsui_2367 at 2519.005 kb on + strand, within Dsui_2367 at 2519.070 kb on + strand, within Dsui_2367 at 2519.103 kb on + strand, within Dsui_2367 at 2519.184 kb on + strand, within Dsui_2367 at 2519.306 kb on + strand, within Dsui_2367 at 2519.370 kb on - strand at 2519.374 kb on + strand at 2519.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,516,833 - -2.4 2,516,930 - -1.2 2,516,993 + Dsui_2365 0.78 -1.0 2,517,276 + Dsui_2365 0.88 -1.4 2,517,277 + Dsui_2365 0.88 +2.5 2,517,304 + Dsui_2365 0.89 -0.1 2,517,323 + Dsui_2365 0.89 -2.0 2,517,350 - -3.2 2,517,350 - -0.8 2,517,512 - +0.3 2,517,527 + -1.3 2,517,535 - +1.0 2,517,762 - +1.6 2,517,762 - +0.8 2,518,638 + -0.0 2,518,648 + +0.3 2,518,680 - -0.7 2,518,725 + Dsui_2367 0.13 +0.0 2,518,730 + Dsui_2367 0.14 +0.9 2,518,740 + Dsui_2367 0.15 +0.9 2,518,839 + Dsui_2367 0.28 +0.2 2,518,884 + Dsui_2367 0.34 +0.7 2,518,968 + Dsui_2367 0.44 -0.2 2,518,979 + Dsui_2367 0.46 +1.4 2,519,005 + Dsui_2367 0.49 +2.1 2,519,070 + Dsui_2367 0.57 -0.1 2,519,103 + Dsui_2367 0.62 +0.9 2,519,184 + Dsui_2367 0.72 +0.3 2,519,306 + Dsui_2367 0.88 +1.0 2,519,370 - -1.2 2,519,374 + +2.1 2,519,450 + +0.3
Or see this region's nucleotide sequence