Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1744 and Dsui_1745 overlap by 4 nucleotides Dsui_1745 and Dsui_1746 overlap by 4 nucleotides Dsui_1746 and Dsui_1747 overlap by 4 nucleotides Dsui_1747 and Dsui_1748 are separated by 202 nucleotides
Dsui_1744: Dsui_1744 - flagellar biosynthetic protein FliR, at 1,833,058 to 1,833,843
_1744
Dsui_1745: Dsui_1745 - flagellar biosynthetic protein FliQ, at 1,833,840 to 1,834,109
_1745
Dsui_1746: Dsui_1746 - flagellar biosynthetic protein FliP, at 1,834,106 to 1,834,858
_1746
Dsui_1747: Dsui_1747 - flagellar biosynthetic protein FliO, at 1,834,855 to 1,835,253
_1747
Dsui_1748: Dsui_1748 - flagellar motor switch protein FliN, at 1,835,456 to 1,835,893
_1748
Position (kb)
1834
1835 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1833.134 kb on + strand at 1833.134 kb on + strand at 1833.134 kb on + strand at 1833.139 kb on + strand, within Dsui_1744 at 1833.142 kb on - strand, within Dsui_1744 at 1833.158 kb on - strand, within Dsui_1744 at 1833.165 kb on + strand, within Dsui_1744 at 1833.165 kb on + strand, within Dsui_1744 at 1833.173 kb on - strand, within Dsui_1744 at 1833.240 kb on + strand, within Dsui_1744 at 1833.285 kb on + strand, within Dsui_1744 at 1833.296 kb on + strand, within Dsui_1744 at 1833.306 kb on + strand, within Dsui_1744 at 1833.325 kb on - strand, within Dsui_1744 at 1833.339 kb on + strand at 1833.347 kb on - strand, within Dsui_1744 at 1833.383 kb on + strand, within Dsui_1744 at 1833.391 kb on - strand, within Dsui_1744 at 1833.444 kb on + strand, within Dsui_1744 at 1833.452 kb on - strand, within Dsui_1744 at 1833.583 kb on - strand, within Dsui_1744 at 1833.639 kb on - strand, within Dsui_1744 at 1833.646 kb on - strand, within Dsui_1744 at 1833.663 kb on + strand, within Dsui_1744 at 1833.827 kb on + strand at 1833.828 kb on + strand at 1833.897 kb on - strand, within Dsui_1745 at 1833.965 kb on + strand, within Dsui_1745 at 1834.037 kb on + strand, within Dsui_1745 at 1834.064 kb on + strand, within Dsui_1745 at 1834.160 kb on - strand at 1834.179 kb on - strand at 1834.345 kb on + strand, within Dsui_1746 at 1834.353 kb on - strand, within Dsui_1746 at 1834.515 kb on + strand, within Dsui_1746 at 1834.515 kb on + strand, within Dsui_1746 at 1834.515 kb on + strand, within Dsui_1746 at 1834.523 kb on - strand, within Dsui_1746 at 1834.523 kb on - strand, within Dsui_1746 at 1834.523 kb on - strand, within Dsui_1746 at 1834.726 kb on + strand, within Dsui_1746 at 1834.728 kb on + strand, within Dsui_1746 at 1834.734 kb on - strand, within Dsui_1746 at 1834.736 kb on - strand, within Dsui_1746 at 1834.785 kb on + strand at 1834.785 kb on + strand at 1834.789 kb on + strand at 1834.888 kb on - strand at 1834.916 kb on + strand, within Dsui_1747 at 1834.937 kb on + strand, within Dsui_1747 at 1834.945 kb on - strand, within Dsui_1747 at 1834.971 kb on - strand, within Dsui_1747 at 1835.096 kb on - strand, within Dsui_1747 at 1835.173 kb on + strand, within Dsui_1747 at 1835.188 kb on + strand, within Dsui_1747 at 1835.208 kb on - strand, within Dsui_1747 at 1835.222 kb on + strand at 1835.232 kb on + strand at 1835.304 kb on - strand at 1835.359 kb on + strand at 1835.372 kb on + strand at 1835.372 kb on + strand at 1835.372 kb on + strand at 1835.380 kb on - strand at 1835.567 kb on + strand, within Dsui_1748 at 1835.576 kb on + strand, within Dsui_1748 at 1835.621 kb on - strand, within Dsui_1748 at 1835.663 kb on + strand, within Dsui_1748 at 1835.693 kb on + strand, within Dsui_1748 at 1835.693 kb on - strand, within Dsui_1748 at 1835.734 kb on - strand, within Dsui_1748
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,833,134 + -0.1 1,833,134 + -0.4 1,833,134 + +0.6 1,833,139 + Dsui_1744 0.10 +1.3 1,833,142 - Dsui_1744 0.11 -4.5 1,833,158 - Dsui_1744 0.13 +1.4 1,833,165 + Dsui_1744 0.14 -2.1 1,833,165 + Dsui_1744 0.14 +1.0 1,833,173 - Dsui_1744 0.15 +1.3 1,833,240 + Dsui_1744 0.23 -0.0 1,833,285 + Dsui_1744 0.29 +1.1 1,833,296 + Dsui_1744 0.30 +0.6 1,833,306 + Dsui_1744 0.32 +0.8 1,833,325 - Dsui_1744 0.34 +1.2 1,833,339 + -1.1 1,833,347 - Dsui_1744 0.37 +1.0 1,833,383 + Dsui_1744 0.41 +1.3 1,833,391 - Dsui_1744 0.42 +0.1 1,833,444 + Dsui_1744 0.49 -0.9 1,833,452 - Dsui_1744 0.50 +1.1 1,833,583 - Dsui_1744 0.67 +1.2 1,833,639 - Dsui_1744 0.74 +0.2 1,833,646 - Dsui_1744 0.75 +1.3 1,833,663 + Dsui_1744 0.77 +0.9 1,833,827 + +0.6 1,833,828 + +0.5 1,833,897 - Dsui_1745 0.21 +1.0 1,833,965 + Dsui_1745 0.46 -0.7 1,834,037 + Dsui_1745 0.73 +0.9 1,834,064 + Dsui_1745 0.83 +0.0 1,834,160 - +1.4 1,834,179 - +1.3 1,834,345 + Dsui_1746 0.32 +0.5 1,834,353 - Dsui_1746 0.33 +0.7 1,834,515 + Dsui_1746 0.54 +1.1 1,834,515 + Dsui_1746 0.54 +1.7 1,834,515 + Dsui_1746 0.54 +0.1 1,834,523 - Dsui_1746 0.55 +1.5 1,834,523 - Dsui_1746 0.55 +0.7 1,834,523 - Dsui_1746 0.55 +0.9 1,834,726 + Dsui_1746 0.82 -0.9 1,834,728 + Dsui_1746 0.83 -1.4 1,834,734 - Dsui_1746 0.83 +1.3 1,834,736 - Dsui_1746 0.84 +1.4 1,834,785 + +0.3 1,834,785 + -1.1 1,834,789 + -0.6 1,834,888 - +0.7 1,834,916 + Dsui_1747 0.15 -0.4 1,834,937 + Dsui_1747 0.21 +0.3 1,834,945 - Dsui_1747 0.23 +1.0 1,834,971 - Dsui_1747 0.29 +1.1 1,835,096 - Dsui_1747 0.60 +1.4 1,835,173 + Dsui_1747 0.80 +1.3 1,835,188 + Dsui_1747 0.83 -0.3 1,835,208 - Dsui_1747 0.88 +2.4 1,835,222 + +0.2 1,835,232 + -1.8 1,835,304 - +0.4 1,835,359 + +0.6 1,835,372 + -0.0 1,835,372 + +0.8 1,835,372 + -1.3 1,835,380 - +0.9 1,835,567 + Dsui_1748 0.25 -0.9 1,835,576 + Dsui_1748 0.27 +0.5 1,835,621 - Dsui_1748 0.38 +0.8 1,835,663 + Dsui_1748 0.47 +0.7 1,835,693 + Dsui_1748 0.54 -0.2 1,835,693 - Dsui_1748 0.54 +1.6 1,835,734 - Dsui_1748 0.63 +1.3
Or see this region's nucleotide sequence