Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1522 and Dsui_1523 are separated by 131 nucleotides Dsui_1523 and Dsui_1524 are separated by 64 nucleotides Dsui_1524 and Dsui_1525 are separated by 87 nucleotides
Dsui_1522: Dsui_1522 - serine/threonine protein kinase, at 1,584,010 to 1,585,344
_1522
Dsui_1523: Dsui_1523 - drug resistance transporter, EmrB/QacA subfamily, at 1,585,476 to 1,586,927
_1523
Dsui_1524: Dsui_1524 - transcriptional regulator, at 1,586,992 to 1,587,495
_1524
Dsui_1525: Dsui_1525 - sulfate/thiosulfate-binding protein, at 1,587,583 to 1,588,590
_1525
Position (kb)
1585
1586
1587 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1584.599 kb on + strand, within Dsui_1522 at 1584.750 kb on + strand at 1584.797 kb on + strand at 1584.805 kb on - strand, within Dsui_1522 at 1584.805 kb on - strand, within Dsui_1522 at 1584.838 kb on - strand, within Dsui_1522 at 1584.908 kb on + strand, within Dsui_1522 at 1584.928 kb on - strand, within Dsui_1522 at 1585.008 kb on - strand, within Dsui_1522 at 1585.018 kb on + strand at 1585.059 kb on + strand, within Dsui_1522 at 1585.120 kb on + strand, within Dsui_1522 at 1585.237 kb on - strand at 1585.324 kb on + strand at 1585.348 kb on - strand at 1585.435 kb on - strand at 1585.447 kb on + strand at 1585.447 kb on + strand at 1585.473 kb on + strand at 1585.518 kb on + strand at 1585.538 kb on - strand at 1585.576 kb on + strand at 1585.654 kb on - strand, within Dsui_1523 at 1585.671 kb on - strand at 1585.689 kb on - strand, within Dsui_1523 at 1585.701 kb on - strand, within Dsui_1523 at 1585.750 kb on - strand at 1585.952 kb on + strand, within Dsui_1523 at 1585.955 kb on - strand, within Dsui_1523 at 1585.981 kb on - strand at 1586.131 kb on - strand, within Dsui_1523 at 1586.274 kb on - strand, within Dsui_1523 at 1586.277 kb on + strand, within Dsui_1523 at 1586.377 kb on + strand, within Dsui_1523 at 1586.490 kb on - strand at 1586.559 kb on + strand at 1586.567 kb on - strand, within Dsui_1523 at 1586.644 kb on + strand at 1586.692 kb on - strand at 1586.751 kb on + strand, within Dsui_1523 at 1586.759 kb on - strand, within Dsui_1523 at 1586.774 kb on + strand at 1586.838 kb on + strand at 1586.846 kb on - strand at 1586.902 kb on - strand at 1586.936 kb on - strand at 1587.079 kb on + strand, within Dsui_1524 at 1587.079 kb on + strand, within Dsui_1524 at 1587.105 kb on + strand, within Dsui_1524 at 1587.189 kb on + strand, within Dsui_1524 at 1587.341 kb on + strand, within Dsui_1524 at 1587.351 kb on - strand, within Dsui_1524 at 1587.351 kb on - strand at 1587.351 kb on - strand at 1587.383 kb on + strand, within Dsui_1524 at 1587.434 kb on + strand, within Dsui_1524 at 1587.541 kb on + strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.549 kb on - strand at 1587.587 kb on - strand at 1587.651 kb on - strand at 1587.677 kb on - strand at 1587.692 kb on + strand, within Dsui_1525 at 1587.700 kb on - strand, within Dsui_1525 at 1587.700 kb on - strand, within Dsui_1525 at 1587.719 kb on - strand, within Dsui_1525 at 1587.757 kb on + strand, within Dsui_1525 at 1587.772 kb on - strand, within Dsui_1525 at 1587.839 kb on + strand, within Dsui_1525 at 1587.839 kb on + strand, within Dsui_1525 at 1587.923 kb on - strand, within Dsui_1525
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,584,599 + Dsui_1522 0.44 +0.3 1,584,750 + -0.7 1,584,797 + +0.5 1,584,805 - Dsui_1522 0.60 +1.9 1,584,805 - Dsui_1522 0.60 +0.7 1,584,838 - Dsui_1522 0.62 +1.5 1,584,908 + Dsui_1522 0.67 +0.6 1,584,928 - Dsui_1522 0.69 +1.0 1,585,008 - Dsui_1522 0.75 +1.1 1,585,018 + -0.2 1,585,059 + Dsui_1522 0.79 +1.2 1,585,120 + Dsui_1522 0.83 +0.6 1,585,237 - +1.9 1,585,324 + +0.0 1,585,348 - +1.8 1,585,435 - -0.2 1,585,447 + -1.5 1,585,447 + -1.8 1,585,473 + -0.4 1,585,518 + -1.4 1,585,538 - -0.4 1,585,576 + -1.0 1,585,654 - Dsui_1523 0.12 +0.1 1,585,671 - +1.7 1,585,689 - Dsui_1523 0.15 -1.8 1,585,701 - Dsui_1523 0.15 -2.0 1,585,750 - +0.9 1,585,952 + Dsui_1523 0.33 -0.2 1,585,955 - Dsui_1523 0.33 +0.1 1,585,981 - +0.9 1,586,131 - Dsui_1523 0.45 +0.5 1,586,274 - Dsui_1523 0.55 +0.3 1,586,277 + Dsui_1523 0.55 -2.2 1,586,377 + Dsui_1523 0.62 -1.0 1,586,490 - -0.4 1,586,559 + +0.5 1,586,567 - Dsui_1523 0.75 +0.7 1,586,644 + -1.0 1,586,692 - +1.0 1,586,751 + Dsui_1523 0.88 -1.1 1,586,759 - Dsui_1523 0.88 +0.8 1,586,774 + -2.3 1,586,838 + -1.0 1,586,846 - +0.1 1,586,902 - +0.4 1,586,936 - +0.2 1,587,079 + Dsui_1524 0.17 +1.4 1,587,079 + Dsui_1524 0.17 +0.8 1,587,105 + Dsui_1524 0.22 +1.1 1,587,189 + Dsui_1524 0.39 +1.0 1,587,341 + Dsui_1524 0.69 +1.2 1,587,351 - Dsui_1524 0.71 -0.1 1,587,351 - -0.7 1,587,351 - -0.5 1,587,383 + Dsui_1524 0.78 +0.9 1,587,434 + Dsui_1524 0.88 +0.8 1,587,541 + -0.9 1,587,549 - -3.5 1,587,549 - -3.4 1,587,549 - -2.7 1,587,549 - -3.0 1,587,549 - -1.2 1,587,549 - -0.4 1,587,587 - +0.4 1,587,651 - -1.6 1,587,677 - -3.3 1,587,692 + Dsui_1525 0.11 -2.6 1,587,700 - Dsui_1525 0.12 -1.0 1,587,700 - Dsui_1525 0.12 -2.6 1,587,719 - Dsui_1525 0.13 -2.2 1,587,757 + Dsui_1525 0.17 +0.3 1,587,772 - Dsui_1525 0.19 -1.8 1,587,839 + Dsui_1525 0.25 -1.1 1,587,839 + Dsui_1525 0.25 -0.3 1,587,923 - Dsui_1525 0.34 -2.3
Or see this region's nucleotide sequence