Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1298 and Dsui_1299 are separated by 69 nucleotides Dsui_1299 and Dsui_1300 are separated by 12 nucleotides Dsui_1300 and Dsui_1301 are separated by 46 nucleotides Dsui_1301 and Dsui_1302 overlap by 4 nucleotides
Dsui_1298: Dsui_1298 - methylase involved in ubiquinone/menaquinone biosynthesis, at 1,361,536 to 1,362,438
_1298
Dsui_1299: Dsui_1299 - putative membrane protein, at 1,362,508 to 1,363,209
_1299
Dsui_1300: Dsui_1300 - sulfite oxidase-like oxidoreductase, at 1,363,222 to 1,364,160
_1300
Dsui_1301: Dsui_1301 - putative transmembrane transcriptional regulator (anti-sigma factor), at 1,364,207 to 1,364,983
_1301
Dsui_1302: Dsui_1302 - RNA polymerase sigma factor, sigma-70 family, at 1,364,980 to 1,365,480
_1302
Position (kb)
1363
1364
1365 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1362.487 kb on - strand at 1362.597 kb on + strand, within Dsui_1299 at 1362.682 kb on - strand, within Dsui_1299 at 1362.684 kb on + strand at 1362.701 kb on + strand, within Dsui_1299 at 1362.709 kb on - strand, within Dsui_1299 at 1362.720 kb on - strand, within Dsui_1299 at 1362.746 kb on + strand, within Dsui_1299 at 1362.812 kb on + strand, within Dsui_1299 at 1362.904 kb on - strand, within Dsui_1299 at 1362.919 kb on - strand, within Dsui_1299 at 1363.052 kb on + strand, within Dsui_1299 at 1363.061 kb on + strand, within Dsui_1299 at 1363.072 kb on + strand at 1363.080 kb on - strand, within Dsui_1299 at 1363.103 kb on - strand, within Dsui_1299 at 1363.124 kb on + strand, within Dsui_1299 at 1363.137 kb on - strand, within Dsui_1299 at 1363.239 kb on + strand at 1363.239 kb on + strand at 1363.247 kb on - strand at 1363.247 kb on - strand at 1363.287 kb on + strand at 1363.288 kb on + strand at 1363.348 kb on + strand at 1363.418 kb on + strand, within Dsui_1300 at 1363.460 kb on - strand, within Dsui_1300 at 1363.566 kb on - strand, within Dsui_1300 at 1363.611 kb on - strand, within Dsui_1300 at 1363.727 kb on - strand, within Dsui_1300 at 1363.747 kb on + strand at 1363.826 kb on + strand at 1363.862 kb on + strand, within Dsui_1300 at 1363.862 kb on + strand at 1363.870 kb on - strand, within Dsui_1300 at 1363.874 kb on + strand, within Dsui_1300 at 1363.908 kb on - strand, within Dsui_1300 at 1363.919 kb on + strand, within Dsui_1300 at 1363.992 kb on - strand, within Dsui_1300 at 1363.999 kb on - strand, within Dsui_1300 at 1364.006 kb on - strand, within Dsui_1300 at 1364.040 kb on + strand at 1364.048 kb on - strand, within Dsui_1300 at 1364.048 kb on - strand, within Dsui_1300 at 1364.050 kb on + strand, within Dsui_1300 at 1364.058 kb on - strand, within Dsui_1300 at 1364.068 kb on + strand at 1364.075 kb on + strand at 1364.075 kb on + strand at 1364.085 kb on + strand at 1364.088 kb on - strand at 1364.093 kb on - strand at 1364.182 kb on - strand at 1364.187 kb on - strand at 1364.197 kb on - strand at 1364.233 kb on + strand at 1364.244 kb on - strand at 1364.254 kb on + strand at 1364.254 kb on + strand at 1364.262 kb on - strand at 1364.262 kb on - strand at 1364.267 kb on - strand at 1364.267 kb on - strand at 1364.267 kb on - strand at 1364.290 kb on - strand, within Dsui_1301 at 1364.381 kb on + strand, within Dsui_1301 at 1364.383 kb on + strand, within Dsui_1301 at 1364.391 kb on - strand, within Dsui_1301 at 1364.557 kb on + strand, within Dsui_1301 at 1364.575 kb on - strand, within Dsui_1301 at 1364.635 kb on - strand, within Dsui_1301 at 1364.640 kb on + strand, within Dsui_1301 at 1364.663 kb on + strand, within Dsui_1301 at 1364.725 kb on + strand, within Dsui_1301 at 1364.738 kb on - strand, within Dsui_1301 at 1364.840 kb on + strand, within Dsui_1301 at 1364.840 kb on + strand, within Dsui_1301 at 1364.840 kb on + strand at 1364.840 kb on + strand, within Dsui_1301 at 1364.845 kb on + strand, within Dsui_1301 at 1364.848 kb on - strand, within Dsui_1301 at 1364.903 kb on + strand, within Dsui_1301 at 1364.945 kb on + strand at 1364.945 kb on + strand at 1364.969 kb on + strand at 1364.992 kb on + strand at 1365.031 kb on + strand, within Dsui_1302 at 1365.099 kb on - strand, within Dsui_1302 at 1365.099 kb on - strand, within Dsui_1302
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,362,487 - +1.0 1,362,597 + Dsui_1299 0.13 -0.7 1,362,682 - Dsui_1299 0.25 -0.3 1,362,684 + -2.0 1,362,701 + Dsui_1299 0.27 -1.5 1,362,709 - Dsui_1299 0.29 -1.3 1,362,720 - Dsui_1299 0.30 -0.8 1,362,746 + Dsui_1299 0.34 -0.5 1,362,812 + Dsui_1299 0.43 -0.5 1,362,904 - Dsui_1299 0.56 -3.1 1,362,919 - Dsui_1299 0.59 +0.2 1,363,052 + Dsui_1299 0.77 -0.6 1,363,061 + Dsui_1299 0.79 -2.2 1,363,072 + -1.9 1,363,080 - Dsui_1299 0.81 +0.3 1,363,103 - Dsui_1299 0.85 +1.6 1,363,124 + Dsui_1299 0.88 -0.8 1,363,137 - Dsui_1299 0.90 -2.7 1,363,239 + -0.4 1,363,239 + -0.3 1,363,247 - +0.3 1,363,247 - +0.9 1,363,287 + -1.8 1,363,288 + -1.2 1,363,348 + -0.4 1,363,418 + Dsui_1300 0.21 -0.8 1,363,460 - Dsui_1300 0.25 -3.4 1,363,566 - Dsui_1300 0.37 -0.2 1,363,611 - Dsui_1300 0.41 -0.6 1,363,727 - Dsui_1300 0.54 -0.4 1,363,747 + +1.5 1,363,826 + -1.8 1,363,862 + Dsui_1300 0.68 -1.1 1,363,862 + -1.9 1,363,870 - Dsui_1300 0.69 -0.2 1,363,874 + Dsui_1300 0.69 -1.2 1,363,908 - Dsui_1300 0.73 -0.2 1,363,919 + Dsui_1300 0.74 -1.6 1,363,992 - Dsui_1300 0.82 +0.2 1,363,999 - Dsui_1300 0.83 -0.5 1,364,006 - Dsui_1300 0.83 -0.1 1,364,040 + -4.4 1,364,048 - Dsui_1300 0.88 -0.4 1,364,048 - Dsui_1300 0.88 -0.4 1,364,050 + Dsui_1300 0.88 -0.3 1,364,058 - Dsui_1300 0.89 +0.5 1,364,068 + -3.6 1,364,075 + -3.8 1,364,075 + -1.0 1,364,085 + -0.1 1,364,088 - -0.4 1,364,093 - -0.1 1,364,182 - -0.3 1,364,187 - +0.5 1,364,197 - +1.6 1,364,233 + -2.3 1,364,244 - -1.2 1,364,254 + -1.9 1,364,254 + -3.3 1,364,262 - -1.2 1,364,262 - -1.4 1,364,267 - -1.6 1,364,267 - -0.1 1,364,267 - -0.5 1,364,290 - Dsui_1301 0.11 -1.8 1,364,381 + Dsui_1301 0.22 -2.0 1,364,383 + Dsui_1301 0.23 -2.0 1,364,391 - Dsui_1301 0.24 -1.3 1,364,557 + Dsui_1301 0.45 -1.9 1,364,575 - Dsui_1301 0.47 -2.0 1,364,635 - Dsui_1301 0.55 -0.8 1,364,640 + Dsui_1301 0.56 -2.1 1,364,663 + Dsui_1301 0.59 -2.1 1,364,725 + Dsui_1301 0.67 -1.4 1,364,738 - Dsui_1301 0.68 -0.1 1,364,840 + Dsui_1301 0.81 -0.7 1,364,840 + Dsui_1301 0.81 +0.0 1,364,840 + -1.7 1,364,840 + Dsui_1301 0.81 -0.1 1,364,845 + Dsui_1301 0.82 -2.2 1,364,848 - Dsui_1301 0.82 -2.9 1,364,903 + Dsui_1301 0.90 -2.8 1,364,945 + -1.8 1,364,945 + -4.8 1,364,969 + -1.5 1,364,992 + -0.1 1,365,031 + Dsui_1302 0.10 +0.3 1,365,099 - Dsui_1302 0.24 -0.3 1,365,099 - Dsui_1302 0.24 +0.4
Or see this region's nucleotide sequence