Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1163 and Dsui_1164 are separated by 49 nucleotides Dsui_1164 and Dsui_1165 overlap by 8 nucleotides
Dsui_1163: Dsui_1163 - hydrogenase accessory protein HypB, at 1,219,522 to 1,220,718
_1163
Dsui_1164: Dsui_1164 - hydrogenase nickel insertion protein HypA, at 1,220,768 to 1,221,112
_1164
Dsui_1165: Dsui_1165 - Ni,Fe-hydrogenase I large subunit, at 1,221,105 to 1,222,094
_1165
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1219.801 kb on + strand at 1219.836 kb on + strand, within Dsui_1163 at 1219.857 kb on + strand at 1219.863 kb on + strand at 1219.929 kb on + strand, within Dsui_1163 at 1219.952 kb on - strand, within Dsui_1163 at 1220.218 kb on + strand, within Dsui_1163 at 1220.236 kb on - strand, within Dsui_1163 at 1220.402 kb on - strand, within Dsui_1163 at 1220.409 kb on - strand, within Dsui_1163 at 1220.414 kb on - strand, within Dsui_1163 at 1220.425 kb on + strand, within Dsui_1163 at 1220.456 kb on - strand, within Dsui_1163 at 1220.470 kb on + strand, within Dsui_1163 at 1220.565 kb on + strand, within Dsui_1163 at 1220.585 kb on - strand, within Dsui_1163 at 1220.610 kb on - strand at 1220.612 kb on + strand at 1220.615 kb on - strand at 1220.683 kb on + strand at 1220.711 kb on + strand at 1220.719 kb on - strand at 1220.724 kb on - strand at 1220.849 kb on + strand at 1220.885 kb on + strand, within Dsui_1164 at 1220.896 kb on + strand, within Dsui_1164 at 1220.927 kb on - strand, within Dsui_1164 at 1220.949 kb on + strand, within Dsui_1164 at 1220.954 kb on + strand at 1220.990 kb on - strand, within Dsui_1164 at 1221.005 kb on - strand, within Dsui_1164 at 1221.035 kb on - strand, within Dsui_1164 at 1221.044 kb on + strand at 1221.052 kb on - strand, within Dsui_1164 at 1221.054 kb on - strand, within Dsui_1164 at 1221.095 kb on - strand at 1221.095 kb on - strand at 1221.138 kb on - strand at 1221.159 kb on + strand at 1221.293 kb on - strand, within Dsui_1165 at 1221.476 kb on + strand at 1221.494 kb on - strand at 1221.603 kb on - strand at 1221.727 kb on + strand at 1221.728 kb on - strand, within Dsui_1165 at 1221.786 kb on + strand, within Dsui_1165 at 1221.904 kb on + strand, within Dsui_1165 at 1221.904 kb on + strand at 1221.917 kb on - strand, within Dsui_1165 at 1222.078 kb on + strand at 1222.078 kb on + strand at 1222.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,219,801 + +0.6 1,219,836 + Dsui_1163 0.26 -0.6 1,219,857 + -0.1 1,219,863 + +1.1 1,219,929 + Dsui_1163 0.34 +0.8 1,219,952 - Dsui_1163 0.36 +1.2 1,220,218 + Dsui_1163 0.58 +0.2 1,220,236 - Dsui_1163 0.60 +0.6 1,220,402 - Dsui_1163 0.74 +0.9 1,220,409 - Dsui_1163 0.74 +1.4 1,220,414 - Dsui_1163 0.75 -0.8 1,220,425 + Dsui_1163 0.75 +0.0 1,220,456 - Dsui_1163 0.78 +0.7 1,220,470 + Dsui_1163 0.79 +0.8 1,220,565 + Dsui_1163 0.87 -0.6 1,220,585 - Dsui_1163 0.89 +0.8 1,220,610 - +0.8 1,220,612 + +1.3 1,220,615 - +0.4 1,220,683 + +0.4 1,220,711 + -3.2 1,220,719 - -0.4 1,220,724 - +0.5 1,220,849 + +1.0 1,220,885 + Dsui_1164 0.34 +0.1 1,220,896 + Dsui_1164 0.37 -3.9 1,220,927 - Dsui_1164 0.46 -0.5 1,220,949 + Dsui_1164 0.52 -0.6 1,220,954 + +1.8 1,220,990 - Dsui_1164 0.64 -0.6 1,221,005 - Dsui_1164 0.69 +0.1 1,221,035 - Dsui_1164 0.77 +0.2 1,221,044 + -0.6 1,221,052 - Dsui_1164 0.82 +0.5 1,221,054 - Dsui_1164 0.83 +1.1 1,221,095 - +0.9 1,221,095 - -0.0 1,221,138 - +0.1 1,221,159 + +0.1 1,221,293 - Dsui_1165 0.19 +0.8 1,221,476 + +0.6 1,221,494 - +0.0 1,221,603 - +0.7 1,221,727 + +1.2 1,221,728 - Dsui_1165 0.63 -0.2 1,221,786 + Dsui_1165 0.69 -0.8 1,221,904 + Dsui_1165 0.81 -1.5 1,221,904 + -0.2 1,221,917 - Dsui_1165 0.82 +0.4 1,222,078 + +0.0 1,222,078 + +0.1 1,222,093 + -0.9
Or see this region's nucleotide sequence