Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0989 and Dsui_0990 are separated by 156 nucleotides Dsui_0990 and Dsui_0991 overlap by 14 nucleotides Dsui_0991 and Dsui_0992 overlap by 20 nucleotides Dsui_0992 and Dsui_0993 overlap by 8 nucleotides
Dsui_0989: Dsui_0989 - amidohydrolase, at 1,016,543 to 1,017,706
_0989
Dsui_0990: Dsui_0990 - putative P-loop-containing kinase, at 1,017,863 to 1,018,735
_0990
Dsui_0991: Dsui_0991 - hypothetical protein, at 1,018,722 to 1,019,150
_0991
Dsui_0992: Dsui_0992 - putative membrane protein, at 1,019,131 to 1,019,706
_0992
Dsui_0993: Dsui_0993 - polyprenyl p-hydroxybenzoate/phenylacrylic acid decarboxylase, at 1,019,699 to 1,020,316
_0993
Position (kb)
1017
1018
1019 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1016.951 kb on + strand, within Dsui_0989 at 1016.956 kb on + strand, within Dsui_0989 at 1016.956 kb on + strand, within Dsui_0989 at 1016.994 kb on + strand at 1017.002 kb on - strand, within Dsui_0989 at 1017.009 kb on + strand, within Dsui_0989 at 1017.034 kb on + strand, within Dsui_0989 at 1017.064 kb on - strand, within Dsui_0989 at 1017.066 kb on + strand, within Dsui_0989 at 1017.233 kb on + strand, within Dsui_0989 at 1017.331 kb on + strand, within Dsui_0989 at 1017.332 kb on - strand, within Dsui_0989 at 1017.566 kb on - strand, within Dsui_0989 at 1017.690 kb on + strand at 1017.708 kb on - strand at 1017.743 kb on + strand at 1017.753 kb on + strand at 1017.804 kb on + strand at 1017.809 kb on + strand at 1017.809 kb on + strand at 1017.809 kb on + strand at 1018.374 kb on + strand, within Dsui_0990 at 1018.764 kb on + strand at 1018.824 kb on - strand at 1018.935 kb on - strand at 1019.036 kb on - strand, within Dsui_0991 at 1019.279 kb on + strand, within Dsui_0992 at 1019.433 kb on + strand, within Dsui_0992 at 1019.488 kb on - strand at 1019.701 kb on + strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,016,951 + Dsui_0989 0.35 +0.3 1,016,956 + Dsui_0989 0.35 +2.5 1,016,956 + Dsui_0989 0.35 +0.7 1,016,994 + -2.3 1,017,002 - Dsui_0989 0.39 -0.0 1,017,009 + Dsui_0989 0.40 +1.7 1,017,034 + Dsui_0989 0.42 +0.0 1,017,064 - Dsui_0989 0.45 +0.5 1,017,066 + Dsui_0989 0.45 +0.7 1,017,233 + Dsui_0989 0.59 +1.6 1,017,331 + Dsui_0989 0.68 +1.0 1,017,332 - Dsui_0989 0.68 +0.8 1,017,566 - Dsui_0989 0.88 +1.2 1,017,690 + -0.8 1,017,708 - +0.1 1,017,743 + +1.3 1,017,753 + -0.8 1,017,804 + +1.4 1,017,809 + +0.7 1,017,809 + -0.5 1,017,809 + -0.3 1,018,374 + Dsui_0990 0.59 -2.0 1,018,764 + +1.4 1,018,824 - -2.5 1,018,935 - -0.9 1,019,036 - Dsui_0991 0.73 -2.9 1,019,279 + Dsui_0992 0.26 -2.4 1,019,433 + Dsui_0992 0.52 -3.9 1,019,488 - -1.8 1,019,701 + +0.3 1,019,709 - +0.6 1,019,709 - +1.0 1,019,709 - -1.6 1,019,709 - +0.2
Or see this region's nucleotide sequence