Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0957 and Dsui_0958 are separated by 64 nucleotides Dsui_0958 and Dsui_0959 are separated by 212 nucleotides Dsui_0959 and Dsui_0960 are separated by 9 nucleotides Dsui_0960 and Dsui_0961 are separated by 96 nucleotides Dsui_0961 and Dsui_0962 are separated by 140 nucleotides
Dsui_0957: Dsui_0957 - nitrogenase molybdenum-iron protein beta chain, at 983,306 to 984,826
_0957
Dsui_0958: Dsui_0958 - ferredoxin, at 984,891 to 985,238
_0958
Dsui_0959: Dsui_0959 - hypothetical protein, at 985,451 to 985,690
_0959
Dsui_0960: Dsui_0960 - putative permease, at 985,700 to 986,755
_0960
Dsui_0961: Dsui_0961 - RNA polymerase sigma factor, SigZ family, at 986,852 to 987,415
_0961
Dsui_0962: Dsui_0962 - collagenase-like protease, at 987,556 to 988,569
_0962
Position (kb) 985
986
987 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 984.731 kb on - strand at 984.738 kb on + strand at 984.738 kb on + strand at 984.857 kb on - strand at 984.913 kb on + strand at 984.931 kb on - strand, within Dsui_0958 at 984.967 kb on - strand, within Dsui_0958 at 985.068 kb on + strand, within Dsui_0958 at 985.073 kb on + strand, within Dsui_0958 at 985.327 kb on + strand at 985.487 kb on - strand, within Dsui_0959 at 985.739 kb on + strand at 985.844 kb on + strand at 985.844 kb on + strand, within Dsui_0960 at 985.849 kb on + strand, within Dsui_0960 at 985.856 kb on + strand, within Dsui_0960 at 985.869 kb on - strand, within Dsui_0960 at 985.932 kb on + strand, within Dsui_0960 at 986.099 kb on + strand at 986.165 kb on + strand, within Dsui_0960 at 986.190 kb on + strand, within Dsui_0960 at 986.237 kb on + strand, within Dsui_0960 at 986.271 kb on - strand, within Dsui_0960 at 986.294 kb on - strand, within Dsui_0960 at 986.438 kb on + strand, within Dsui_0960 at 986.593 kb on + strand, within Dsui_0960 at 986.611 kb on + strand, within Dsui_0960 at 986.852 kb on - strand at 986.927 kb on + strand at 986.975 kb on + strand, within Dsui_0961 at 986.996 kb on - strand, within Dsui_0961 at 987.008 kb on - strand, within Dsui_0961 at 987.216 kb on - strand, within Dsui_0961 at 987.228 kb on + strand at 987.316 kb on + strand, within Dsui_0961 at 987.324 kb on - strand, within Dsui_0961 at 987.436 kb on + strand at 987.480 kb on - strand at 987.524 kb on - strand at 987.524 kb on - strand at 987.599 kb on + strand at 987.721 kb on + strand, within Dsui_0962
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 984,731 - +0.4 984,738 + +0.2 984,738 + +0.3 984,857 - +0.2 984,913 + +1.6 984,931 - Dsui_0958 0.11 +0.1 984,967 - Dsui_0958 0.22 -0.1 985,068 + Dsui_0958 0.51 +1.1 985,073 + Dsui_0958 0.52 +0.3 985,327 + +0.6 985,487 - Dsui_0959 0.15 +0.2 985,739 + +0.9 985,844 + +2.4 985,844 + Dsui_0960 0.14 -0.4 985,849 + Dsui_0960 0.14 +0.1 985,856 + Dsui_0960 0.15 +1.0 985,869 - Dsui_0960 0.16 +0.2 985,932 + Dsui_0960 0.22 +0.4 986,099 + +1.2 986,165 + Dsui_0960 0.44 +1.8 986,190 + Dsui_0960 0.46 -0.5 986,237 + Dsui_0960 0.51 +0.4 986,271 - Dsui_0960 0.54 +1.5 986,294 - Dsui_0960 0.56 -0.1 986,438 + Dsui_0960 0.70 +0.2 986,593 + Dsui_0960 0.85 +0.4 986,611 + Dsui_0960 0.86 +2.4 986,852 - -0.1 986,927 + +2.0 986,975 + Dsui_0961 0.22 -2.1 986,996 - Dsui_0961 0.26 -0.1 987,008 - Dsui_0961 0.28 -0.4 987,216 - Dsui_0961 0.65 +0.0 987,228 + +1.0 987,316 + Dsui_0961 0.82 -0.2 987,324 - Dsui_0961 0.84 +1.1 987,436 + +0.1 987,480 - +0.5 987,524 - +0.9 987,524 - -1.0 987,599 + -2.9 987,721 + Dsui_0962 0.16 -6.1
Or see this region's nucleotide sequence