Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0701 and Dsui_0702 overlap by 32 nucleotides Dsui_0702 and Dsui_0703 are separated by 155 nucleotides Dsui_0703 and Dsui_0704 are separated by 14 nucleotides Dsui_0704 and Dsui_0705 are separated by 66 nucleotides Dsui_0705 and Dsui_0706 are separated by 65 nucleotides
Dsui_0701: Dsui_0701 - serine kinase of the HPr protein, regulates carbohydrate metabolism, at 696,888 to 697,823
_0701
Dsui_0702: Dsui_0702 - PTS IIA-like nitrogen-regulatory protein PtsN, at 697,792 to 698,277
_0702
Dsui_0703: Dsui_0703 - ribosomal subunit interface protein, at 698,433 to 698,753
_0703
Dsui_0704: Dsui_0704 - RNA polymerase sigma-54 factor, at 698,768 to 700,246
_0704
Dsui_0705: Dsui_0705 - ABC-type (unclassified) transport system, ATPase component, at 700,313 to 701,059
_0705
Dsui_0706: Dsui_0706 - hypothetical protein, at 701,125 to 701,580
_0706
Position (kb) 698
699
700
701 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1 at 698.421 kb on - strand at 698.479 kb on - strand, within Dsui_0703 at 698.493 kb on + strand, within Dsui_0703 at 698.613 kb on - strand, within Dsui_0703 at 698.613 kb on - strand, within Dsui_0703 at 698.640 kb on - strand, within Dsui_0703 at 698.647 kb on - strand, within Dsui_0703 at 698.676 kb on - strand, within Dsui_0703 at 698.719 kb on + strand, within Dsui_0703 at 698.802 kb on - strand at 698.816 kb on + strand at 698.837 kb on + strand at 698.847 kb on - strand at 698.850 kb on - strand at 699.035 kb on - strand, within Dsui_0704 at 699.035 kb on - strand, within Dsui_0704 at 699.058 kb on + strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.081 kb on - strand, within Dsui_0704 at 699.226 kb on + strand at 699.356 kb on - strand, within Dsui_0704 at 699.417 kb on - strand, within Dsui_0704 at 699.442 kb on - strand, within Dsui_0704 at 699.493 kb on + strand, within Dsui_0704 at 699.511 kb on - strand, within Dsui_0704 at 699.609 kb on - strand, within Dsui_0704 at 699.678 kb on - strand, within Dsui_0704 at 699.683 kb on - strand, within Dsui_0704 at 699.709 kb on - strand, within Dsui_0704 at 699.928 kb on + strand, within Dsui_0704 at 699.928 kb on + strand, within Dsui_0704 at 699.967 kb on + strand, within Dsui_0704 at 700.040 kb on - strand, within Dsui_0704 at 700.200 kb on - strand at 700.212 kb on - strand at 700.282 kb on + strand at 700.302 kb on + strand at 701.087 kb on - strand at 701.100 kb on + strand at 701.109 kb on - strand at 701.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 698,421 - -1.1 698,479 - Dsui_0703 0.14 -3.6 698,493 + Dsui_0703 0.19 -4.4 698,613 - Dsui_0703 0.56 -1.7 698,613 - Dsui_0703 0.56 -2.1 698,640 - Dsui_0703 0.64 -3.6 698,647 - Dsui_0703 0.67 -2.1 698,676 - Dsui_0703 0.76 -2.1 698,719 + Dsui_0703 0.89 -3.4 698,802 - -2.5 698,816 + -4.0 698,837 + -3.1 698,847 - -7.9 698,850 - -5.9 699,035 - Dsui_0704 0.18 -3.1 699,035 - Dsui_0704 0.18 -5.2 699,058 + Dsui_0704 0.20 -4.8 699,081 - Dsui_0704 0.21 -4.8 699,081 - Dsui_0704 0.21 -6.2 699,081 - Dsui_0704 0.21 -4.6 699,226 + -0.3 699,356 - Dsui_0704 0.40 -1.1 699,417 - Dsui_0704 0.44 -2.9 699,442 - Dsui_0704 0.46 -5.0 699,493 + Dsui_0704 0.49 -4.8 699,511 - Dsui_0704 0.50 -5.1 699,609 - Dsui_0704 0.57 -3.9 699,678 - Dsui_0704 0.62 -7.2 699,683 - Dsui_0704 0.62 -5.6 699,709 - Dsui_0704 0.64 -5.4 699,928 + Dsui_0704 0.78 -1.9 699,928 + Dsui_0704 0.78 -3.6 699,967 + Dsui_0704 0.81 -4.0 700,040 - Dsui_0704 0.86 -5.4 700,200 - -5.8 700,212 - -5.8 700,282 + -3.4 700,302 + -5.1 701,087 - -0.7 701,100 + +0.4 701,109 - -1.6 701,135 + -0.1
Or see this region's nucleotide sequence