Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0681 and Dsui_0682 are separated by 161 nucleotides Dsui_0682 and Dsui_0683 are separated by 161 nucleotides Dsui_0683 and Dsui_0684 are separated by 105 nucleotides
Dsui_0681: Dsui_0681 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 678,563 to 679,372
_0681
Dsui_0682: Dsui_0682 - 5,10-methenyltetrahydrofolate synthetase, at 679,534 to 680,127
_0682
Dsui_0683: Dsui_0683 - soluble lytic murein transglycosylase-like protein, at 680,289 to 682,277
_0683
Dsui_0684: Dsui_0684 - putative nucleoside-diphosphate sugar epimerase, at 682,383 to 683,342
_0684
Position (kb) 680
681
682
683 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 679.331 kb on + strand at 679.339 kb on - strand at 680.121 kb on - strand at 680.173 kb on + strand at 680.181 kb on - strand at 680.186 kb on - strand at 680.254 kb on + strand at 680.257 kb on - strand at 680.299 kb on + strand at 680.306 kb on - strand at 680.338 kb on + strand at 680.346 kb on - strand at 680.502 kb on - strand, within Dsui_0683 at 680.525 kb on + strand, within Dsui_0683 at 680.545 kb on - strand at 680.593 kb on - strand at 680.642 kb on + strand, within Dsui_0683 at 680.738 kb on + strand, within Dsui_0683 at 680.804 kb on - strand, within Dsui_0683 at 680.816 kb on - strand, within Dsui_0683 at 680.899 kb on - strand at 680.913 kb on + strand, within Dsui_0683 at 680.913 kb on + strand, within Dsui_0683 at 680.913 kb on + strand, within Dsui_0683 at 680.918 kb on + strand, within Dsui_0683 at 681.007 kb on - strand, within Dsui_0683 at 681.150 kb on + strand, within Dsui_0683 at 681.239 kb on + strand, within Dsui_0683 at 681.276 kb on - strand at 681.312 kb on - strand, within Dsui_0683 at 681.386 kb on + strand, within Dsui_0683 at 681.526 kb on + strand, within Dsui_0683 at 681.593 kb on + strand, within Dsui_0683 at 681.671 kb on - strand, within Dsui_0683 at 681.727 kb on + strand, within Dsui_0683 at 681.757 kb on - strand, within Dsui_0683 at 681.861 kb on - strand, within Dsui_0683 at 681.948 kb on - strand, within Dsui_0683 at 681.952 kb on + strand, within Dsui_0683 at 681.962 kb on + strand, within Dsui_0683 at 681.991 kb on + strand, within Dsui_0683 at 682.015 kb on - strand, within Dsui_0683 at 682.168 kb on - strand at 682.198 kb on - strand at 682.234 kb on - strand at 682.308 kb on + strand at 682.328 kb on - strand at 682.355 kb on - strand at 682.356 kb on + strand at 682.361 kb on - strand at 682.369 kb on + strand at 682.377 kb on - strand at 682.518 kb on + strand, within Dsui_0684 at 682.664 kb on + strand, within Dsui_0684 at 682.672 kb on - strand at 682.750 kb on - strand, within Dsui_0684 at 682.768 kb on + strand, within Dsui_0684 at 682.881 kb on + strand, within Dsui_0684 at 682.891 kb on + strand, within Dsui_0684 at 682.899 kb on - strand, within Dsui_0684 at 682.901 kb on + strand, within Dsui_0684 at 682.909 kb on - strand, within Dsui_0684 at 682.914 kb on - strand, within Dsui_0684 at 682.917 kb on + strand, within Dsui_0684 at 682.974 kb on + strand, within Dsui_0684 at 683.001 kb on - strand at 683.011 kb on + strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.023 kb on + strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.043 kb on + strand, within Dsui_0684 at 683.046 kb on - strand, within Dsui_0684 at 683.047 kb on + strand, within Dsui_0684 at 683.135 kb on + strand, within Dsui_0684
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 679,331 + +0.5 679,339 - +0.8 680,121 - -1.1 680,173 + -0.4 680,181 - +0.7 680,186 - +0.3 680,254 + +0.3 680,257 - -0.7 680,299 + -0.7 680,306 - -0.4 680,338 + -0.1 680,346 - -1.4 680,502 - Dsui_0683 0.11 -0.6 680,525 + Dsui_0683 0.12 +1.2 680,545 - -3.5 680,593 - -1.2 680,642 + Dsui_0683 0.18 -0.4 680,738 + Dsui_0683 0.23 -1.1 680,804 - Dsui_0683 0.26 -2.0 680,816 - Dsui_0683 0.26 -1.0 680,899 - -1.6 680,913 + Dsui_0683 0.31 -1.9 680,913 + Dsui_0683 0.31 -0.5 680,913 + Dsui_0683 0.31 -1.1 680,918 + Dsui_0683 0.32 +0.2 681,007 - Dsui_0683 0.36 -1.6 681,150 + Dsui_0683 0.43 -1.2 681,239 + Dsui_0683 0.48 -0.9 681,276 - -3.5 681,312 - Dsui_0683 0.51 -0.6 681,386 + Dsui_0683 0.55 -0.1 681,526 + Dsui_0683 0.62 -0.1 681,593 + Dsui_0683 0.66 -0.8 681,671 - Dsui_0683 0.69 -1.2 681,727 + Dsui_0683 0.72 -0.4 681,757 - Dsui_0683 0.74 -0.5 681,861 - Dsui_0683 0.79 +0.3 681,948 - Dsui_0683 0.83 +1.7 681,952 + Dsui_0683 0.84 +0.6 681,962 + Dsui_0683 0.84 -1.4 681,991 + Dsui_0683 0.86 +0.4 682,015 - Dsui_0683 0.87 +0.4 682,168 - -2.9 682,198 - -1.8 682,234 - -1.7 682,308 + -1.4 682,328 - -0.9 682,355 - -1.3 682,356 + +0.6 682,361 - -0.3 682,369 + +0.4 682,377 - -0.7 682,518 + Dsui_0684 0.14 -1.9 682,664 + Dsui_0684 0.29 -0.9 682,672 - -1.4 682,750 - Dsui_0684 0.38 -3.1 682,768 + Dsui_0684 0.40 -4.4 682,881 + Dsui_0684 0.52 -0.0 682,891 + Dsui_0684 0.53 -0.1 682,899 - Dsui_0684 0.54 -2.8 682,901 + Dsui_0684 0.54 -0.1 682,909 - Dsui_0684 0.55 -2.5 682,914 - Dsui_0684 0.55 +0.3 682,917 + Dsui_0684 0.56 +0.1 682,974 + Dsui_0684 0.62 -0.8 683,001 - +0.0 683,011 + Dsui_0684 0.65 -0.5 683,019 - Dsui_0684 0.66 -3.8 683,019 - Dsui_0684 0.66 -2.1 683,023 + Dsui_0684 0.67 -2.1 683,031 - Dsui_0684 0.68 -1.0 683,031 - Dsui_0684 0.68 -1.9 683,043 + Dsui_0684 0.69 +0.0 683,046 - Dsui_0684 0.69 -2.9 683,047 + Dsui_0684 0.69 +0.3 683,135 + Dsui_0684 0.78 +0.8
Or see this region's nucleotide sequence