Experiment: spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0574 and Dsui_0575 are separated by 12 nucleotides Dsui_0575 and Dsui_0576 are separated by 21 nucleotides Dsui_0576 and Dsui_0577 overlap by 8 nucleotides
Dsui_0574: Dsui_0574 - flavin-dependent dehydrogenase, at 571,880 to 573,178
_0574
Dsui_0575: Dsui_0575 - electron transfer flavoprotein, alpha subunit, at 573,191 to 574,273
_0575
Dsui_0576: Dsui_0576 - electron transfer flavoprotein, beta subunit, at 574,295 to 575,140
_0576
Dsui_0577: Dsui_0577 - Nitrogen fixation protein NifW, at 575,133 to 575,465
_0577
Position (kb) 573
574
575 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 572.192 kb on + strand, within Dsui_0574 at 572.303 kb on - strand, within Dsui_0574 at 572.303 kb on - strand at 572.321 kb on - strand, within Dsui_0574 at 572.339 kb on - strand, within Dsui_0574 at 572.430 kb on + strand at 572.505 kb on + strand, within Dsui_0574 at 572.638 kb on - strand, within Dsui_0574 at 572.645 kb on - strand at 572.793 kb on - strand, within Dsui_0574 at 572.821 kb on - strand at 572.838 kb on - strand, within Dsui_0574 at 572.887 kb on - strand, within Dsui_0574 at 572.977 kb on + strand, within Dsui_0574 at 573.091 kb on + strand at 573.099 kb on - strand at 573.121 kb on - strand at 573.231 kb on - strand at 573.255 kb on + strand at 573.332 kb on - strand, within Dsui_0575 at 573.334 kb on + strand, within Dsui_0575 at 573.484 kb on + strand, within Dsui_0575 at 573.716 kb on - strand, within Dsui_0575 at 573.737 kb on - strand, within Dsui_0575 at 573.864 kb on - strand, within Dsui_0575 at 573.904 kb on + strand at 573.964 kb on + strand, within Dsui_0575 at 574.022 kb on - strand, within Dsui_0575 at 574.065 kb on - strand, within Dsui_0575 at 574.077 kb on + strand, within Dsui_0575 at 574.095 kb on + strand, within Dsui_0575 at 574.155 kb on - strand, within Dsui_0575 at 574.202 kb on - strand at 574.273 kb on + strand at 574.283 kb on + strand at 574.301 kb on - strand at 574.341 kb on + strand at 574.344 kb on - strand at 574.349 kb on - strand at 574.360 kb on - strand at 574.362 kb on + strand at 574.461 kb on - strand, within Dsui_0576 at 574.519 kb on + strand, within Dsui_0576 at 574.591 kb on - strand, within Dsui_0576 at 574.592 kb on - strand, within Dsui_0576 at 575.106 kb on + strand at 575.116 kb on + strand at 575.124 kb on - strand at 575.135 kb on + strand at 575.150 kb on + strand at 575.150 kb on + strand at 575.195 kb on - strand, within Dsui_0577
Per-strain Table
Position Strand Gene LocusTag Fraction spiked with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 572,192 + Dsui_0574 0.24 +0.0 572,303 - Dsui_0574 0.33 +0.5 572,303 - +1.1 572,321 - Dsui_0574 0.34 -0.5 572,339 - Dsui_0574 0.35 +0.2 572,430 + -0.9 572,505 + Dsui_0574 0.48 -1.5 572,638 - Dsui_0574 0.58 -0.1 572,645 - +2.2 572,793 - Dsui_0574 0.70 +0.6 572,821 - -1.0 572,838 - Dsui_0574 0.74 +0.3 572,887 - Dsui_0574 0.78 +0.4 572,977 + Dsui_0574 0.84 +1.3 573,091 + +0.6 573,099 - -6.6 573,121 - +0.6 573,231 - +0.6 573,255 + +0.5 573,332 - Dsui_0575 0.13 -0.5 573,334 + Dsui_0575 0.13 -0.0 573,484 + Dsui_0575 0.27 +0.6 573,716 - Dsui_0575 0.48 -0.1 573,737 - Dsui_0575 0.50 +0.2 573,864 - Dsui_0575 0.62 +0.6 573,904 + +0.3 573,964 + Dsui_0575 0.71 -1.7 574,022 - Dsui_0575 0.77 +1.7 574,065 - Dsui_0575 0.81 +0.0 574,077 + Dsui_0575 0.82 -0.8 574,095 + Dsui_0575 0.83 -3.8 574,155 - Dsui_0575 0.89 -0.0 574,202 - -0.2 574,273 + -0.3 574,283 + +0.1 574,301 - -1.7 574,341 + +1.5 574,344 - +0.3 574,349 - -2.0 574,360 - +0.3 574,362 + +0.3 574,461 - Dsui_0576 0.20 +0.6 574,519 + Dsui_0576 0.26 -1.4 574,591 - Dsui_0576 0.35 +0.6 574,592 - Dsui_0576 0.35 -0.8 575,106 + +0.9 575,116 + -0.7 575,124 - +0.5 575,135 + -0.0 575,150 + -0.9 575,150 + +0.9 575,195 - Dsui_0577 0.19 -0.2
Or see this region's nucleotide sequence